公开(公告)号：US20170276922A1

公开(公告)日：2017-09-28

申请号：US15362125

申请日：2016-11-28

Applicant: NIKON CORPORATION

Inventor： Tatsushi NOMURA ,  Hisao OSAWA ,  Naoki FUKUTAKE

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G01N21/6456 ,  G02B21/14

Abstract: In order to realize a high-speed switching of structured pattern, a structured illuminating microscopy includes a driving unit generating a sonic standing wave in a sonic wave propagation path by giving a driving signal for vibrating a medium of the sonic wave propagation path to a light modulator, an illuminating optical system making at least three diffracted components of the exit light flux passed through the sonic wave propagation path to be interfered with one another, and forming interference fringes on an observational object, an image-forming optical system forming an image by an observational light flux from the observational object on a detector, and a controlling unit controlling a contrast of an image by modulating at least one of an intensity of the exit light flux, an intensity of the observational light flux, and the detector with a modulating signal of 1/N frequency of the driving signal.

公开(公告)号：US20160289671A1

公开(公告)日：2016-10-06

申请号：US14914373

申请日：2014-08-05

Applicant: THE UNIVERSITY OF TOKYO ,  NIKON CORPORATION

Inventor： Takanori ICHIKI ,  Shingo UENO ,  Hisao OSAWA

IPC: C12N15/10

CPC classification number: C12N15/1075 ,  C07K1/04 ,  C07K17/14 ,  C40B30/04 ,  G01N33/6842 ,  G01N33/6845

Abstract: There is provided a method of manufacturing a protein array or peptide array suitable for an efficient screening of a functional protein or functional peptide. The method of manufacturing a protein array or peptide array includes the steps of: (a) preparing a nucleic acid immobilized on a solid support and a cell-free synthesis system in a reactor, in which a reactor array includes the reactor having a specific aperture shape and a protein capture molecule or a peptide capture molecule provided on at least a portion of wall surface and bottom surface in the reactor; and (c) synthesizing a protein or peptide from the nucleic acid using the cell-free synthesis system and immobilizing the protein or peptide in the reactor.

Abstract translation: 提供了一种制备适于有效筛选功能性蛋白质或功能性肽的蛋白质阵列或肽阵列的方法。 制备蛋白质阵列或肽阵列的方法包括以下步骤：（a）在反应器中制备固定在固体支持物上的核酸和无细胞合成系统，其中反应器阵列包括具有特定孔径的反应器 形状和蛋白质捕获分子或肽捕获分子，其设置在反应器的壁表面和底表面的至少一部分上; 和（c）使用无细胞合成系统从核酸合成蛋白质或肽，并将蛋白质或肽固定在反应器中。

公开(公告)号：US20160062102A1

公开(公告)日：2016-03-03

申请号：US14834755

申请日：2015-08-25

Applicant: NIKON CORPORATION ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Yumiko OUCHI ,  Hisao OSAWA ,  Mats G. L. GUSTAFSSON ,  David A. AGARD

IPC: G02B21/36 ,  H04N7/18 ,  G02B5/30 ,  G02B21/06 ,  G02B27/10

CPC classification number: G02B21/367 ,  G02B5/3083 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/361 ,  G02B27/1066 ,  G02B27/58 ,  H04N7/18

Abstract: Polarized light which is emitted from an optical fiber becomes circular polarized light by passing through a first quarter wave plate. The circular polarized light which has entered a second quarter wave plate is converted into nearly linear polarized light which has S polarization. P polarization components are removed from the nearly linear polarized light by a polarizer, but the polarizer is not always necessary. The optical axis of the polarizer is set to be a direction which allows transmitting of S polarized light. The light that has passed through the polarizer is separated into diffracted lights by a diffraction grating, and is used as the structured illumination light.

Abstract translation: 从光纤发射的偏振光通过第一四分之一波片变成圆偏振光。 进入第二四分之一波片的圆偏振光转换为具有S偏振的近似线偏振光。 通过偏振器从近似线偏振光中去除P偏振分量，但不一定需要偏振器。 偏振器的光轴被设定为允许透射S偏振光的方向。 已经通过偏振器的光被衍射光栅分离成衍射光，并被用作结构照明光。

公开(公告)号：US20150292862A1

公开(公告)日：2015-10-15

申请号：US14751771

申请日：2015-06-26

Applicant: NIKON CORPORATION

Inventor： Ryoichi SATAKA ,  Hisao OSAWA

IPC: G01B9/04 ,  G01J3/02

CPC classification number: G01B9/04 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0216 ,  G01J3/2803 ,  G01J3/36 ,  G01J3/4406 ,  G02B21/0064

Abstract: A spectroscope including: a spectral element that is configured to spectrally separate signal light; a first optical system that is configured to condense spectroscopic light spectrally separated by the spectral element; and an optical receiver that is configured to receive the spectroscopic light; wherein the optical receiver includes a plurality of regions different sensitivities with respect to a wavelength characteristics of the spectroscopic light.

Abstract translation: 一种分光镜，包括：被配置为光谱分离信号光的光谱元件; 第一光学系统，被配置为使由所述光谱元件光谱分离的分光光阑聚集; 以及被配置为接收所述分光光的光接收器; 其中所述光学接收器包括多个区域相对于所述分光光度的波长特性而具有不同的灵敏度。

公开(公告)号：US20140055594A1

公开(公告)日：2014-02-27

申请号：US14068799

申请日：2013-10-31

Applicant: Nikon Corporation

Inventor： Tatsushi NOMURA ,  Hisao OSAWA ,  Naoki FUKUTAKE

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G01N21/6456 ,  G02B21/14

Abstract: In order to realize a high-speed switching of structured pattern, a structured illuminating microscopy includes a driving unit generating a sonic standing wave in a sonic wave propagation path by giving a driving signal for vibrating a medium of the sonic wave propagation path to a light modulator, an illuminating optical system making at least three diffracted components of the exit light flux passed through the sonic wave propagation path to be interfered with one another, and forming interference fringes on an observational object, an image-forming optical system forming an image by an observational light flux from the observational object on a detector, and a controlling unit controlling a contrast of an image by modulating at least one of an intensity of the exit light flux, an intensity of the observational light flux, and the detector with a modulating signal of 1/N frequency of the driving signal.

Abstract translation: 为了实现结构图案的高速切换，结构化照明显微镜包括驱动单元，其通过给出将声波传播路径的介质振动到光的驱动信号，在声波传播路径中产生声波驻波 调制器，使得通过声波传播路径的出射光束的至少三个衍射分量彼此干涉并在观测对象上形成干涉条纹的照明光学系统，形成图像的图像形成光学系统 来自检测器上的观测对象的观测光通量，以及控制单元，其通过调制出出光束的强度，观察光束的强度和检测器的强度中的至少一个来调制图像的对比度， 信号为驱动信号的1 / N频率。