Abstract:
The present invention relates to an immunogenic conjugate comprising a carrier molecule coupled to an autoinducer of a Gram negative bacteria The immunogenic conjugate, when combined with a pharmaceutically acceptable carrier, forms a suitable vaccine for mammals to prevent infection by the Gram negative bacteria The immunogenic conjugate is also used to raise and subsequently isolate antibodies or binding portions thereof which are capable of recognizing and binding to the autoinducer. The antibodies or binding portions thereof are utilized in a method of treating infections, a method of inhibiting autoinducer activity, and in diagnostic assays which detect the presence of autoinducers or autoinducer antagonists in fluid or tissue samples. TABLE OF CONTENTS Page 1. FIELD OF THE INVENTION1 2. BACKGROUND OF THE INVENTION1 3. SUMMARY OF THE INVENTION4 4. BRIEF DESCRIPTION OF THE DRAWINGS7 5. DETAILED DESCRIPTION OF THE INVENTION7 5.1 Isolation and Synthesis of Homoserine Lactones7 5.2 Bacterial Autoinducer Conjugation to a Carrier17 5.3 Generation of Antibodies to BAI Immunogenic conjugates19 5.4 Therapeutic Uses of Immunogenic conjugates22 or Antibodies Thereto 5.4.1 Administration and Formulation22 5.4.2 Effective Dose25 5.5 Diagnostic Methods27 6. EXAMPLE: SYNTHESIS32 Preparation of Compound C32 Preparation of Compound D32 Preparation of Compound 532 Preparation of Compound 633 Preparation of Compound 733 Preparation of Compound 833 Preparation of Compound 1034 Preparation of Compound 1134 Preparation of Compound 1335 Preparation of Compound 1235 Preparation of Compound 1435 Preparation of Compound 1536 Preparation of Compound 1736 Preparation of Compound 2137 Preparation of Compound 2237 Preparation of Compound 2438 Preparation of Compound 2538 Preparation of Compound 2738 Preparation of Compound 2939 7. EXAMPLE: PRODUCTION OF BAI ANTIBODIES39 7.1 PAI'S Role In Virulence39 7.2 Anti-PAI Polyclonal Antibodies40 7.3 Neutralization of PAI With Antibodies40
Abstract:
A contact lens package has a body with a lens receptacle therein covered by a hinged lid. The lid is offset on the body such that a ledge is formed by the base and an overhang by the lid. The ledge has a plurality of upstanding lugs that insert into mating receptacles formed in the overhang of an adjacent contact lens package, permitting the ganging of packages together for functional and aesthetic reasons.
Abstract:
A vehicle for separating a mixed debris stream is disclosed. The vehicle includes an elongate vehicle frame; a separating assembly configured to contact the mixed debris stream with a fluid stream to separate the mixed debris stream into at least a first debris stream and a second debris stream, and at least one of the first debris stream and the second debris stream includes fluid from the fluid stream; a first conveyor; a second conveyor; and a fluid collection structure configured to receive fluid from at least the separating assembly and the second conveyor, wherein the first conveyor is configured to drain at least some fluid from the first debris stream, to the second conveyor, and the second conveyor includes a non-mesh conveyor belt configured to drain at least some fluid from at least one of the first debris stream and the second debris stream to the fluid collection structure.
Abstract:
A computing hardware and software device called a Meta Mentor Central Processing Unit. The Meta Mentor purpose is to control memory, input/output interfaces, defining the operating system, the scheduling processes and the parsing of individual threads to an array of slave Processing Units that are uniquely connected, thus creating a highly optimized computing core. In effect, the Meta Mentor tells the array of slave processors what to process by controlling all external and internal system input/output and individual processor scheduling.
Abstract translation:称为Meta Mentor中央处理单元的计算硬件和软件设备。 Meta Mentor的目的是控制内存,输入/输出接口,定义操作系统,调度过程和将各个线程解析为独立连接的从属处理单元阵列,从而创建高度优化的计算核心。 实际上,Meta Mentor通过控制所有外部和内部系统输入/输出以及单独的处理器调度来告知从属处理器阵列的处理过程。
Abstract:
A bead lock including an insert casing and a bead spacer for providing an air passageway in a vehicle tire to be mounted on a wheel rim on which is located an insert valve and a rim lip for retention of the vehicle tire bead, the insert casing including an insert bead adapted to push against the tire bead and to hold the tire bead firmly against the inner surface of the rim lip, the inner wall of the vehicle tire and the outer surface of the insert casing defining an internal tire space to be pressurized for inflation of the vehicle tire or depressurized for the deflation of the vehicle tire via the insert valve, the tire bead and the insert bead interposed between the internal tire space and the insert valve, the bead spacer extending from the insert bead to an insert valve area corresponding in use to the insert valve location whereby to define at least one air passage for communication of the internal tire space with the insert valve, wherein the bead spacer is made of material sufficiently flexible to substantially conform to the contours of the wheel rim and sufficiently incompressible to provide the air passageway.
Abstract:
A package for contact lenses includes a first panel having a housing mechanism, such as receptacles or wells, for housing a set of contact lenses in the first panel and a covering layer removably attached to the first panel for enclosing the set of contact lenses in the housing mechanism. The package also includes a second panel attached to the first panel. The second panel is pivotable relative to the first panel between a first position, in which the second panel is folded onto the first panel, and a second position, in which the second panel is pivoted away from the first panel. When the second panel is positioned in its first position, the first and second panels form a substantially flat package which is adapted for display on a conventional display unit.
Abstract:
Described herein are methods for separating one or more analytes present in a fluid sample. The methods involve passing the fluid through a filter having a microporous material, wherein the analytes are localized near the surface of the microporous material. Additional processing steps such as hybridization and amplification can be performed once the analyte is localized. In one method, once the analyte is localized, the analyte can be detected, counted, and correlated in order to determine the concentration of the analyte in the sample. Modified microporous materials and composite materials are also disclosed that can be used in any of the methods and articles described herein. The composite is composed of a microporous material and a pigment, wherein the pigment is incorporated in the microporous material. The pigments alter the optical properties of the microporous material, which enhances the detection of analyte once it is localized. In a further aspect, various kits and articles such as filtration devices containing any of the microporous materials described herein are provided.
Abstract:
A container with an interior and a neck finish has a recessed pocket on an exterior side of a sidewall for snap fitting a digital disk thereon. Opposing flanges at the periphery of the pocket snap fit the disk in place in a generally vertical orientation.
Abstract:
A bead lock including an insert casing and a bead spacer for providing an air passageway in a vehicle tire to be mounted on a wheel rim on which is located an insert valve and a rim lip for retention of the vehicle tire bead, said insert casing including an insert bead adapted to push against said tire bead and to hold said tire bead firmly against the inner surface of said rim lip, the inner wall of said vehicle tire and the outer surface of said insert casing defining an internal tire space to be pressurized for inflation of said vehicle tire or depressurized for the deflation of said vehicle tire via said insert valve, said tire bead and said insert bead interposed between said internal tire space and said insert valve, said bead spacer extending from said insert bead to an insert valve area corresponding in use to said insert valve location whereby to define at least one air passage for communication of said internal tire space with said insert valve, wherein said bead spacer is made of material sufficiently flexible to substantially conform to the contours of said wheel rim and sufficiently incompressible to provide said air passageway.
Abstract:
This invention relates to novel diaryl ureas, pharmaceutical compositions containing such compounds and the use of those compounds or compositions for treating hyper-proliferative and angiogenesis disorders, as a sole agent or in combination with cytotoxic therapies.