公开(公告)号：US20220058776A1

公开(公告)日：2022-02-24

申请号：US17418782

申请日：2019-12-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yair Rivenson ,  Yichen Wu

IPC: G06T5/00 ,  G06T3/40 ,  G06T5/50 ,  G06K9/62 ,  G01N21/64 ,  G06N3/08

Abstract: A fluorescence microscopy method includes a trained deep neural network. At least one 2D fluorescence microscopy image of a sample is input to the trained deep neural network, wherein the input image(s) is appended with a digital propagation matrix (DPM) that represents, pixel-by-pixel, an axial distance of a user-defined or automatically generated surface within the sample from a plane of the input image. The trained deep neural network outputs fluorescence output image(s) of the sample that is digitally propagated or refocused to the user-defined surface or automatically generated. The method and system cross-connects different imaging modalities, permitting 3D propagation of wide-field fluorescence image(s) to match confocal microscopy images at different sample planes, The method may be used to output a time sequence of images (e.g., time-lapse video) of a 2D or 3D surface within a sample.

公开(公告)号：US20200310100A1

公开(公告)日：2020-10-01

申请号：US16300539

申请日：2017-05-09

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Yibo Zhang ,  Yichen Wu

IPC: G02B21/36 ,  G03H1/04 ,  G02B21/00 ,  G03H1/08 ,  G06T5/50

Abstract: Methods and systems for generating a high-color-fidelity and high-resolution color image of a sample are disclosed; which fuses or merges a holographic image acquired at a single wavelength with a color-calibrated image taken by a low-magnification lens-based microscope using a wavelet transform based colorization method. A holographic microscope is used to obtain holographic images which are used to computationally reconstruct a high-resolution mono-color holographic image of the sample. A lens-based microscope is used to obtain low resolution color images. A discrete wavelet transform (DWT) is used to generate a final image that merges the low-resolution components from the lens-based color image and the high-resolution components from the high-resolution mono-color holographic image.