Patent search ap:"Klint A. Rose" Page 3

21.

发明申请
METHODS FOR SEPARATING PARTICLES AND/OR NUCLEIC ACIDS USING ISOTACHOPHORESIS 有权
Title translation: 使用异烟肼分离颗粒和/或核酸的方法

公开(公告)号：US20110036718A1

公开(公告)日：2011-02-17

申请号：US12542215

申请日：2009-08-17

Applicant: Byoungsok Jung , Kevin Ness , Klint A. Rose

Inventor： Byoungsok Jung , Kevin Ness , Klint A. Rose

IPC: G01N27/447

CPC classification number: G01N27/44747 , G01N27/44773 , G01N2030/8827

Abstract: According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

Abstract translation: 根据一个实施方案，一种方法包括将包含前导电解质，尾电解质和至少一种DNA和RNA的流体的流体共同输送到通道，以及在垂直于诱导横向等电泳的通道。在另一个实施例中，一种方法包括将流体共同输送到通道。流体包括前导电解质，尾电解质，生物物体，DNA和RNA中的至少一种以及具有电泳迁移率的间隔电解质，所述间隔电解质位于至少一些生物体的电泳迁移率与电泳迁移率之间至少一个DNA和RNA。该方法还包括在垂直于通道的轴线的方向上向流体施加电场以诱导横向等速电泳。除此之外还公开了其他的等速电泳方法。

22.

发明授权
Microfluidic systems with embedded materials and structures and method thereof 失效
Title translation: 具有嵌入材料和结构的微流体系统及其方法

公开(公告)号：US07186352B2

公开(公告)日：2007-03-06

申请号：US10853859

申请日：2004-05-25

Applicant: Jeffrey D. Morse , Klint A Rose , Mariam Maghribi , William Benett , Peter Krulevitch , Julie Hamilton , Robert T. Graff , Alan Jankowski

Inventor： Jeffrey D. Morse , Klint A Rose , Mariam Maghribi , William Benett , Peter Krulevitch , Julie Hamilton , Robert T. Graff , Alan Jankowski

IPC: H01M8/00

CPC classification number: B01D67/0034 , B01D65/003 , B01D67/0032 , B01D71/70 , B01D2323/24 , B01D2325/028 , B01D2325/08 , H01M8/0239 , H01M8/0245 , H01M8/04037 , H01M8/04089 , H01M8/1007 , H01M8/1097 , Y10T29/49114

Abstract: Described herein is a process for fabricating microfluidic systems with embedded components in which micron-scale features are molded into the polymeric material polydimethylsiloxane (PDMS). Micromachining is used to create a mold master and the liquid precursors for PDMS are poured over the mold and allowed to cure. The PDMS is then removed form the mold and bonded to another material such as PDMS, glass, or silicon after a simple surface preparation step to form sealed microchannels.

Abstract translation: 本文描述了一种用于制造具有嵌入组件的微流体系统的方法，其中将微米级特征模制成聚合物材料聚二甲基硅氧烷（PDMS）。微加工用于制造模具主体，将PDMS的液体前体倒在模具上并使其固化。然后在简单的表面制备步骤之后，从模具中除去PDMS并结合到另一种材料如PDMS，玻璃或硅，以形成密封的微通道。