公开(公告)号：US5998134A

公开(公告)日：1999-12-07

申请号：US482627

申请日：1995-06-07

Applicant: Wen-Hwa Lee ,  Eva Y-H. P. Lee

Inventor： Wen-Hwa Lee ,  Eva Y-H. P. Lee

IPC: A61K38/00 ,  C07K14/47 ,  C12Q1/68

CPC classification number: C07K14/4746 ,  A61K38/00 ,  C12N2799/026

Abstract: This invention relates in general to a genetic control of retinoblastoma and regulation of carcinogenicity. In particular this invention relates to the cloning, isolation, identification and sequencing of the retinoblastoma gene. The invention also relates to the method of use of the cloned retinoblastoma gene cDNA as a tool for diagnosing retinoblastoma, osteosarcoma and fibrosarcoma. Finally, the invention relates to the control and regulatory mechanisms and functions of the retinoblastoma gene in the oncogenesis.

Abstract translation: 本发明一般涉及视网膜母细胞瘤的遗传控制和致癌性的调节。 特别地，本发明涉及视网膜母细胞瘤基因的克隆，分离，鉴定和测序。 本发明还涉及使用克隆的视网膜母细胞瘤基因cDNA作为诊断视网膜母细胞瘤，骨肉瘤和纤维肉瘤的工具的方法。 最后，本发明涉及肿瘤发生中视网膜母细胞瘤基因的控制和调节机制和功能。

公开(公告)号：US5851991A

公开(公告)日：1998-12-22

申请号：US306513

申请日：1994-09-13

Applicant: Wen-Hwa Lee ,  Eva Y-H.P. Lee ,  David W. Goodrich ,  H. Michael Shepard ,  Nan Ping Wang ,  Duane Johnson

Inventor： Wen-Hwa Lee ,  Eva Y-H.P. Lee ,  David W. Goodrich ,  H. Michael Shepard ,  Nan Ping Wang ,  Duane Johnson

IPC: A61K38/00 ,  C07K14/47 ,  A61K38/17

CPC classification number: C07K14/4746 ,  A61K38/00 ,  C12N2501/998 ,  C12N2510/00 ,  C12N2799/026

Abstract: This invention provides a method of preventing or inhibiting the proliferation of a pathologically proliferating cell, wherein the pathological proliferation of the cell is the result of the absence of a functional retinoblastoma protein or polypeptide in the cell. The method requires contacting the cell with an effective amount of retinoblastoma protein or polypeptide. This method also is useful to prevent or treat retinoblastoma or a secondary cancer to retinoblastoma by administering to a patient a functional retinoblastoma protein or polypeptide.

Abstract translation: 本发明提供了预防或抑制病理增殖细胞增殖的方法，其中细胞的病理性增殖是细胞中不存在功能性视网膜母细胞瘤蛋白或多肽的结果。 该方法需要使细胞与有效量的视网膜母细胞瘤蛋白质或多肽接触。 该方法还可用于通过向患者施用功能性视网膜母细胞瘤蛋白或多肽来预防或治疗视网膜母细胞瘤或次要癌症的视网膜母细胞瘤。

公开(公告)号：US4942123A

公开(公告)日：1990-07-17

申请号：US98612

申请日：1987-09-17

Applicant: Wen-Hwa Lee ,  Eva Y. - H. P. Lee

Inventor： Wen-Hwa Lee ,  Eva Y. - H. P. Lee

IPC: A61K38/00 ,  C07K14/47 ,  G01N33/567 ,  G01N33/574

CPC classification number: C07K14/4746 ,  G01N33/567 ,  G01N33/5748 ,  A61K38/00 ,  C12N2799/026 ,  Y10S436/813 ,  Y10S530/828

Abstract: This invention relates in general to a phosphoprotein product of the retinoblastoma susceptibility gene. In particular, this invention relates to a phosphoprotein ppRB.sup.110 primarily located in the cell nucleus which has a DNA binding activity. The invention also relates to the amino acid sequence of the phosphoprotein and to the specific purified anti-retinoblastoma phosphoprotein antibody. The invention further relates to a method of diagnosing retinoblastoma and other retinoblastoma gene involved cancers, treating such kind of cancers and regulating the oncogenicity of other genes.

Abstract translation: 本发明一般涉及视网膜母细胞瘤易感基因的磷蛋白产物。 特别地，本发明涉及主要位于具有DNA结合活性的细胞核中的磷蛋白ppRB110。 本发明还涉及磷蛋白和特异性纯化的抗视网膜母细胞瘤磷蛋白抗体的氨基酸序列。 本发明还涉及诊断视网膜母细胞瘤和其他视网膜母细胞瘤基因涉及的癌症，治疗这种癌症并调节其他基因的致癌性的方法。