公开(公告)号：US20230074185A1

公开(公告)日：2023-03-09

申请号：US17933832

申请日：2022-09-20

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU

IPC: F27D1/12 ,  F27B3/14 ,  F27B3/24 ,  F27D11/08

Abstract: Immunogenic compositions comprising hemagglutinin (HA) variants and/or neuraminidase (NA) variants, which may be contained in an influenza A virus, and uses thereof for eliciting immune responses against influenza A virus.

公开(公告)号：US20210332146A1

公开(公告)日：2021-10-28

申请号：US17211619

申请日：2021-03-24

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Ming-Hung Tsai

IPC: C07K16/28 ,  C12P21/00

Abstract: The present disclosure relates to a novel class of anti-CD20 monoclonal antibodies comprising a homogeneous population of anti-CD20 IgG molecules having the same N-glycan on each of Fc. The antibodies of the invention can be produced from anti-CD20 monoclonal antibodies by Fc glycoengineering. Importantly, the antibodies of the invention have improved therapeutic values with increased ADCC activity and increased Fc receptor binding affinity compared to the corresponding monoclonal antibodies that have not been glycoengineered.

公开(公告)号：US20190194303A1

公开(公告)日：2019-06-27

申请号：US16150164

申请日：2018-10-02

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU

IPC: C07K16/18 ,  C12P21/00

CPC classification number: C07K16/18 ,  C07K2317/41 ,  C07K2317/50 ,  C12P21/005 ,  C12Y204/01 ,  C12Y204/99003

Abstract: Methods for making modified Fc regions of antibodies and antibody fragments, both human and humanized, and having enhanced stability and efficacy, are provided. Antibodies comprising Fc regions with core fucose residues removed, and attached to oligosaccharides comprising terminal sialyl residues, are provided. Antibodies comprising homogeneous glycosylation of Fc regions with specific oligosaccharides are provided. Fc regions conjugated with homogeneous glycoforms of monosaccharides and trisaccharides, are provided. Methods of preparing human antibodies with modified Fc using glycan engineering, are provided.

公开(公告)号：US20180011104A1

公开(公告)日：2018-01-11

申请号：US15666242

申请日：2017-08-01

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Jim-Min FANG ,  Jiun-Jie SHIE

IPC: G01N33/58 ,  C07F5/02 ,  G01N21/64 ,  C07D309/14 ,  C07K7/08 ,  C07D311/02

CPC classification number: G01N33/582 ,  C07D309/14 ,  C07D311/02 ,  C07F5/027 ,  C07K7/08 ,  G01N21/64 ,  G01N2333/924 ,  G01N2458/30

Abstract: Provided are azido-BODIPY compounds of formula (I), cyclooctyne-based fluorogenic probes of formula (IV), and activity-based probes of formula (VI). These compounds undergo azide-alkyne cycloadditions (AAC) with to form triazolyl products. The provided compounds are useful for detection and imaging of alkyne-, or azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.

公开(公告)号：US20170360924A1

公开(公告)日：2017-12-21

申请号：US15689165

申请日：2017-08-29

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Alice L. YU ,  Kun-Hsien LIN ,  Tai-Na WU

IPC: A61K39/39 ,  C07H15/18 ,  A61K39/00

CPC classification number: A61K39/39 ,  A61K2039/55511 ,  A61K2039/57 ,  C07H15/18

Abstract: Glycosphingolipids (GSLs) bearing α-glucose (α-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with α-glucose (α-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with α-galactose (α-Gal) are disclosed. GSLs bearing α-glucose (α-Glc) and derivatives of α-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with α-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with α-Glc and derivatives thereof are provided.

公开(公告)号：US20160280794A1

公开(公告)日：2016-09-29

申请号：US15011544

申请日：2016-01-30

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU ,  Che MA ,  Han-Chung WU

IPC: C07K16/30 ,  C07K16/40 ,  C07K16/24 ,  C07K16/28

CPC classification number: C07K16/30 ,  A61K45/06 ,  C07K16/1018 ,  C07K16/18 ,  C07K16/241 ,  C07K16/2887 ,  C07K16/32 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2319/00 ,  C12N9/24 ,  C12P21/005 ,  C12Y302/01 ,  C12Y302/01051

Abstract: The present disclosure relates to compositions and methods of use comprising antibodies or binding fragments thereof further comprising universal Fc glycoforms.

Abstract translation: 本公开涉及包含抗体或其结合片段的组合物和使用方法，其还包含通用Fc糖形式。

公开(公告)号：US20160017390A1

公开(公告)日：2016-01-21

申请号：US14722612

申请日：2015-05-27

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Tsung-I TSAI

IPC: C12P19/14 ,  C12N9/24

CPC classification number: C12P19/14 ,  A61K39/3955 ,  A61K39/42 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/1018 ,  C07K16/18 ,  C07K16/241 ,  C07K16/2887 ,  C07K16/2896 ,  C07K16/30 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/72 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  C12N9/24 ,  C12Y302/01 ,  C12Y302/01051 ,  Y02A50/412 ,  Y02A50/466

Abstract: The present disclosure relates to an α-fucosidase having α-(1,2), α-(1,3), α-(1,4), and α-(1,6) fucosidase activity. The present disclosure also relates to the compositions comprising the α-fucosidase, and the methods of producing and using the α-fucosidase in cleaving α-(1,2), α-(1,3), α-(1,4), and/or α-(1,6)-linked fucoses in the glycoconjugates.

Abstract translation: 本公开涉及具有α-（1,2），α-（1,3），α-（1,4）和α-（1,6）岩藻糖苷酶活性的α-岩藻糖苷酶。 本公开还涉及包含α-岩藻糖苷酶的组合物，以及α-裂解α-（1,2），α-（1,3），α-（1,4）， ，和/或α-（1,6）连接的岩藻糖。

公开(公告)号：US20150344587A1

公开(公告)日：2015-12-03

申请号：US14723181

申请日：2015-05-27

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU

IPC: C07K16/40 ,  C12P21/00 ,  A61K39/395 ,  C07K16/28 ,  A61K31/517

CPC classification number: C07K16/40 ,  A61K31/517 ,  A61K39/3955 ,  C07K16/283 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/40 ,  C07K2317/41 ,  C07K2317/732 ,  C07K2317/92 ,  C12P21/005 ,  C12Y302/01 ,  C12Y302/01051

Abstract: The present disclosure relates to a novel class of anti-HER2 monoclonal antibodies comprising a homogeneous population of anti-HER2 IgG molecules having the same N-glycan on each of Fc. The antibodies of the invention can be produced from anti-HER2 monoclonal antibodies by Fc glycoengineering. Importantly, the antibodies of the invention have improved therapeutic values with increased ADCC activity and increased Fc receptor binding affinity compared to the corresponding monoclonal antibodies that have not been glycoengineered.

Abstract translation: 本公开涉及一类新的抗HER2单克隆抗体，其包含在Fc上具有相同N-聚糖的均匀抗HER2 IgG分子群。 本发明的抗体可以通过Fc糖工程从抗HER2单克隆抗体产生。 重要的是，本发明的抗体具有改善的治疗价值，与尚未糖基化的相应单克隆抗体相比，具有增加的ADCC活性和增加的Fc受体结合亲和力。

公开(公告)号：US20150344559A1

公开(公告)日：2015-12-03

申请号：US14722775

申请日：2015-05-27

Applicant: Academia Sinica

Inventor： Chi-Huey WONG ,  Chung-Yi WU

IPC: C07K16/24 ,  A61K39/395 ,  A61K45/06 ,  C12P21/00

Abstract: The present disclosure relates to a novel class of anti-TNFα monoclonal antibodies or antigen binding fragments comprising a homogeneous population of anti-TNFα IgG molecules having the same N-glycan on each of Fc. The antibodies of the invention can be produced from anti-TNFα monoclonal antibodies by Fc glycoengineering. The glycoantibodies of the invention may have improved therapeutic values compared to the corresponding monoclonal antibodies that have not been glycoengineered.

Abstract translation: 本公开内容涉及一类新的抗TNFα单克隆抗体或抗原结合片段，其包含在Fc上具有相同N-聚糖的均匀抗TNFαIgG分子群。 本发明的抗体可以通过Fc糖工程从抗TNFα单克隆抗体产生。 本发明的糖抗体与相对于尚未糖基化的单克隆抗体相比可具有改善的治疗价值。

公开(公告)号：US20150094237A1

公开(公告)日：2015-04-02

申请号：US14565323

申请日：2014-12-09

Applicant: Academia Sinica

Inventor： Pi-Hui LIANG ,  Chi-Huey WONG

IPC: G01N33/53

CPC classification number: G01N33/5308 ,  B01J19/0046 ,  C40B30/04 ,  C40B40/12 ,  G01N2400/00 ,  G01N2400/10

Abstract: A method, system and device to identify, study and/or mimic carbohydrate-protein interactions on cell surfaces and in solution measured by a glycan microarray. In some instances the method, system and device uses very small quantities of carbohydrate as low as attomol. In some instances the system, method and device is high-throughput. The small quantity senstivity may allow for close placement of carbohydrate array members wherein due to close proximity multivalent interactions with proteins may be identified.

Abstract translation: 鉴定，研究和/或模拟细胞表面和聚糖微阵列测量溶液中碳水化合物 - 蛋​​白质相互作用的方法，系统和装置。 在某些情况下，该方法，系统和装置使用非常少量的碳水化合物，与阿托莫尔一样低。 在某些情况下，系统，方法和设备是高吞吐量的。 可以鉴定少量的灵敏度可以容易地放置碳水化合物阵列成员，其中由于与蛋白质的近似多价相互作用可以被鉴定。