公开(公告)号：US11008157B2

公开(公告)日：2021-05-18

申请号：US15419678

申请日：2017-01-30

Applicant: CORNING INCORPORATED

Inventor： Jessica Monique Brown ,  Nicholas Michael Illsley ,  Gregory Roger Martin ,  Ana Marie del Pilar Pardo ,  Allison Jean Tanner

IPC: B65D83/00 ,  A01N1/02 ,  A61J1/14 ,  A61J1/05 ,  A61J1/16 ,  B65D81/32 ,  B65D21/02 ,  C12M1/26 ,  C12M1/00 ,  C12M3/00 ,  A61M5/145 ,  A61M5/24

Abstract: An article including either a frame having an interior cavity, and a flexible liner situated within the interior cavity; or a deformable frame having an interior cavity. Either frame can receive a sample, such as liquid having suspended live cells, and cooling freezes the sample to a solid sample. The solid sample can be readily ejected from the article having either a frame with the flexible liner, or the deformable frame, without extensive thawing. The solid sample can be rapidly displaced from the article.

公开(公告)号：US20210062126A1

公开(公告)日：2021-03-04

申请号：US16629665

申请日：2018-07-13

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/22 ,  C12M1/00

Abstract: A microcavity dish (10) for cultivating cells includes a dish body including a sidewall (16) that encloses a cell culture chamber within the dish body. The dish body has a top and a bottom (12). The bottom includes a cell culturing substrate comprising an array of microcavities (46). The sidewall includes a transition portion (30) that divides the sidewall into an upper portion and a lower portion that is offset inward relative to the upper portion defining a liquid medium delivery surface (26) that extends at least partially along an interior surface (28) of the sidewall and slopes toward the bottom.

公开(公告)号：US20200148990A1

公开(公告)日：2020-05-14

申请号：US16569852

申请日：2019-09-13

Applicant: Corning Incorporated

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/32 ,  C12M3/06 ,  C12M1/00 ,  C12M1/12 ,  C12M3/04 ,  C12M1/14

Abstract: A cell culture apparatus includes one or more plates having a first major surface and an opposing second major surface. The first major surface comprises a structured surface defining a plurality of wells. Each well has an interior surface defining an upper aperture and a nadir, wherein the upper aperture of each well has a diametric dimension in a range from 100 micrometers to 2000 micrometers. The apparatus also includes a plurality of spacers extending from the first major surface along a length of the bottom surface. A plurality of flow channels are defined between adjacent rails.

公开(公告)号：US20180242573A1

公开(公告)日：2018-08-30

申请号：US15774883

申请日：2016-11-03

Applicant: Corning Incorporated

Inventor： William Joseph Lacey ,  Gregory Roger Martin ,  Allison Jean Tanner

IPC: A01N1/02 ,  B01L3/00

CPC classification number: A01N1/0268 ,  B01L3/5082 ,  B01L3/50825 ,  B01L2200/141 ,  B01L2300/042 ,  B01L2300/043 ,  B01L2300/046 ,  B01L2300/0609 ,  B01L2300/0832 ,  B01L2300/0858 ,  B01L2300/123 ,  C12M45/22

Abstract: Vial assemblies comprise a tubular body and a cap, the cap including a first portion configured to abut a lip of an open end of the tubular body, a threaded portion configured to couple to threading on an internal surface of the tubular body, and a second portion protruding from the threaded portion and extending into a cavity of the tubular body. Methods for storing and removing frozen samples from such vial assemblies are also described.

公开(公告)号：US09845451B2

公开(公告)日：2017-12-19

申请号：US14812671

申请日：2015-07-29

Applicant: Corning Incorporated

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M3/02 ,  C12M1/24 ,  C12M1/12 ,  C12M1/00 ,  C12M1/04 ,  C12N5/071

CPC classification number: C12M23/08 ,  C12M23/04 ,  C12M23/24 ,  C12M23/34 ,  C12M23/38 ,  C12M23/58 ,  C12M25/02 ,  C12M25/04 ,  C12M25/06 ,  C12M29/04 ,  C12N5/0602

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

公开(公告)号：US20170267959A1

公开(公告)日：2017-09-21

申请号：US15528644

申请日：2015-11-20

Applicant: Corning Incorporated

Inventor： Gregory Roger Martin ,  Allison Jean Tanner ,  Alexander Meyer Wilks

IPC: C12M1/24 ,  C12N5/071 ,  C12M1/00

CPC classification number: C12M23/08 ,  C12M23/20 ,  C12N5/0682

Abstract: This disclosure provides a cell culture media extending material capable of releasing nutrients into the cell culture environment slowly over time. In embodiments, this material is a part of a cell culture vessel. In embodiments, the material is a coating or a film on a surface of a cell culture material. In additional embodiments, the material is a surface upon which cells are cultured, such as a cell culture vessel or a microcarrier.

公开(公告)号：US20150361388A1

公开(公告)日：2015-12-17

申请号：US14768272

申请日：2014-02-26

Applicant: Gregory Roger MARTIN ,  Allison Jean TANNER ,  CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/26 ,  C12N13/00 ,  C12M1/32 ,  C12M1/24 ,  C12M1/22

CPC classification number: C12M33/00 ,  C12M23/08 ,  C12M23/10 ,  C12M23/12 ,  C12M33/08 ,  C12N13/00

Abstract: An apparatus (100) for cell release from a cell culture article (101), including: an ultrasonic source (105); a stage comprising a base (112) and a raised portion (114) that defines a cell culture article confinement area; and a mechanism for relative scan motion between the ultrasonic source and the cell culture article confined to the stage. Also disclosed is a method for cell release and harvesting from a cell culture article (101), including: securing a cell culture article in the disclosed cell release apparatus; and accomplishing relative motion scanning between the energized ultrasonic source and the cell culture article for a time, as defined herein.

Abstract translation: 一种用于从细胞培养物品（101）释放细胞的装置（100），包括：超声波源（105）; 包括限定细胞培养物品限制区域的基部（112）和凸起部分（114）的台阶; 以及超声波源和限于该阶段的细胞培养物之间的相对扫描运动的机制。 还公开了从细胞培养物（101）进行细胞释放和收获的方法，包括：确保细胞培养物品在所公开的细胞释放装置中; 并且如本文所定义的那样，在通电的超声波源和细胞培养物品之间完成一段时间的相对运动扫描。

公开(公告)号：US20140322806A1

公开(公告)日：2014-10-30

申请号：US14087906

申请日：2013-11-22

Applicant: Corning Incorporated

Inventor： Scott Matthew Bennett ,  Brian Robb Douglass ,  Paul Ernest Gagnon, JR. ,  Gregory Roger Martin ,  Paul Michael Szlosek ,  Allison Jean Tanner

IPC: C12N5/071

CPC classification number: C12N5/0602 ,  C12M23/02 ,  C12M23/12 ,  C12M23/24 ,  C12M25/04 ,  C12M29/04

Abstract: A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

Abstract translation: 1.一种球状细胞培养物，其特征在于，具有：具有室的框架，具有：不透明侧壁面; 顶孔; 透气透明的底部; 并且可选地包括室附件表面和第二底部，并且透明底部的至少一部分包括至少一个凹弧形表面。 还公开了制造和使用该制品的方法。

公开(公告)号：US11976263B2

公开(公告)日：2024-05-07

申请号：US17691511

申请日：2022-03-10

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/32 ,  C12M1/12 ,  C12M3/00

CPC classification number: C12M25/04 ,  C12M21/08 ,  C12M23/12

Abstract: A cell culture insert for use in culturing cells to promote the formation of spheroids and methods of using these spheroid-promoting cell culture inserts. The cell culture insert includes a porous membrane and one or more sidewalls that are non-adherent to cells and cause the cells in the insert to associate with each other and form spheroids.

公开(公告)号：US20240091778A1

公开(公告)日：2024-03-21

申请号：US18525119

申请日：2023-11-30

Applicant: CORNING INCORPORATED

Inventor： William Joseph Lacey ,  Gregory Roger Martin ,  Ana Maria del Pilar Pardo ,  Allison Jean Tanner

IPC: B01L3/00 ,  C12M1/00 ,  C12M1/24

CPC classification number: B01L3/5085 ,  C12M23/08 ,  C12M23/38 ,  B01L2300/0858

Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.