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公开(公告)号：US20080012193A1
公开(公告)日：2008-01-17
申请号：US11822337
申请日：2007-07-05
Applicant: Daisuke Ejima , Tsuyoshi Kida
Inventor： Daisuke Ejima , Tsuyoshi Kida
IPC: B23Q3/00
CPC classification number: F27D5/0037 , H01L21/673 , H01L24/97 , H01L2224/16 , H01L2224/16245 , H01L2224/45144 , H01L2224/48227 , H01L2224/48465 , H01L2224/73204 , H01L2224/73265 , H01L2924/00011 , H01L2924/00014 , H01L2924/181 , H01L2924/00 , H01L2224/0401
Abstract: A heat treatment jig includes a holding concave portion to hold an object and a void part formed near the holding concave portion and a thermal history variation of the object in a heat treatment process is reduced thereby.
Abstract translation: 热处理夹具包括用于保持物体的保持凹部和形成在保持凹部附近的空隙部，并且由此降低热处理工序中的物体的热历史变化。
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公开(公告)号：US06833258B2
公开(公告)日：2004-12-21
申请号：US09892864
申请日：2001-06-28
Applicant: Keiichi Yokoyama , Kunio Ono , Daisuke Ejima
Inventor： Keiichi Yokoyama , Kunio Ono , Daisuke Ejima
IPC: C12N910
CPC classification number: C12N9/1044 , C12Y203/02013
Abstract: The present invention relates to a high-yield process for producing high-activity, high-purity transglutaminase by refolding denatured microorganism-derived transglutaminase by reacting denatured transglutaminase in an aqueous medium under acidic conditions and reconstituting a higher-order enzymatically active state in an aqueous medium at a neutral pH.
Abstract translation: 本发明涉及一种生产高活性，高纯度转谷氨酰胺酶的高产率方法，其通过在酸性条件下将变性转谷氨酰胺酶在水性介质中反应并重新折叠变性微生物转谷氨酰胺酶，并在水性中重新构建高级酶活性状态 培养基处于中性pH值。
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公开(公告)号：US5610284A
公开(公告)日：1997-03-11
申请号：US275663
申请日：1994-07-15
Applicant: Daisuke Ejima , Yutaka Sato , Mayumi Watanabe , Masayo Date , Yoshiyuki Takahara
Inventor： Daisuke Ejima , Yutaka Sato , Mayumi Watanabe , Masayo Date , Yoshiyuki Takahara
CPC classification number: C07K14/5412 , A61K38/00
Abstract: There are disclosed (i) a purification process for obtaining a human BCDF having the intramolecular disulfide linkage and the stereostructure of natural type human BCDF which comprises subjecting to an oxidation reaction and a refolding treatment a reduced type human BCDF obtained by culturing a microorganism having a human BCDF gene integrated therein and solubilized with guanidine hydrochloride, characterized in that after the oxidation reaction, a gel filtration chromatographic treatment is conducted under the conditions of the guanidine hydrochloride concentration adjusted to 4-7M; (ii) a purification process for obtaining a natural type human BCDF monomer by removing the organic solvent from an organic solvent-containing solution of human BCDF, characterized in that the solution is passed through a gel filtration chromatographic column equilibrated with an organic solvent, followed by eluting according to a stepwise or linear gradient program; and (iii) a human BCDF purification process comprising an ion exchange chromatographic treatment and a reversed phase HPLC step, in combination (i) or (ii). According to these purification processes, it becomes possible to remove the impurities derived from a microorganism and human BCDF analogs, and the thus obtained natural type human BCDF has a high purity and can be utilized for pharmaceutical preparations.
Abstract translation: 公开了（i）用于获得具有分子内二硫键的人BCDF和天然型人BCDF的立体结构的纯化方法，其包括进行氧化反应和重折叠处理，所述还原型人BCDF通过培养具有 人BCDF基因整合在其中并用盐酸胍溶解，其特征在于，在氧化反应后，在盐酸胍浓度调节至4-7M的条件下进行凝胶过滤色谱处理; （ii）通过从含有机溶剂的人BCDF中除去有机溶剂来获得天然型人BCDF单体的纯化方法，其特征在于使溶液通过用有机溶剂平衡的凝胶过滤色谱柱，随后 通过逐步或线性梯度程序洗脱; 和（iii）包含离子交换色谱处理和反相HPLC步骤（组合（i）或（ii））的人BCDF纯化方法。 根据这些纯化方法，可以除去由微生物和人BCDF类似物衍生的杂质，由此得到的天然型人BCDF具有高纯度，可用于药物制剂。
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公开(公告)号：US09872499B2
公开(公告)日：2018-01-23
申请号：US13591839
申请日：2012-08-22
Applicant: Daisuke Ejima , Haruna Sato , Hajime Koyama , Tsutomu Arakawa
Inventor： Daisuke Ejima , Haruna Sato , Hajime Koyama , Tsutomu Arakawa
IPC: A01N47/44 , A61K31/192 , A61K31/198 , A61K31/222 , A61K31/341 , A61K31/352 , A61K36/82 , A61K45/06
CPC classification number: A01N47/44 , A61K31/192 , A61K31/198 , A61K31/222 , A61K31/341 , A61K31/352 , A61K36/82 , A61K45/06 , A01N2300/00 , A61K2300/00 , A01N37/38 , A01N43/08 , A01N43/16 , A01N43/90 , A01N65/08
Abstract: The invention provides a virus-inactivating composition with pH of 3.8 to 5.5, containing (A) 0.02 to 0.3 M arginine and (B) a component such as 0.01 to 10 mM flavonoid, polyphenol, or ascorbic acid derivative, 0.005 to 5 mass % of an arginine derivative, and 0.1 to 2.5 mass % of an extract solution of natural product.
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Patent Agency Ranking
公开(公告)号：US08470328B2
公开(公告)日：2013-06-25
申请号：US13533238
申请日：2012-06-26
Applicant: Ryosuke Yumioka , Daisuke Ejima , Tsutomu Arakawa
Inventor： Ryosuke Yumioka , Daisuke Ejima , Tsutomu Arakawa
IPC: A61K39/395
CPC classification number: C07K16/065 , C07K1/22 , C07K16/2845 , C07K16/36 , C07K2317/24
Abstract: The present invention provides a method of purifying an antibody by protein A affinity chromatography. More specifically, the present invention provides a technique relating to an elution buffer solution which provides a good antibody recovery rate without denaturation.
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公开(公告)号：US08084032B2
公开(公告)日：2011-12-27
申请号：US11030088
申请日：2005-01-07
Applicant: Ryosuke Yumioka , Daisuke Ejima , Tsutomu Arakawa
Inventor： Ryosuke Yumioka , Daisuke Ejima , Tsutomu Arakawa
IPC: A61K39/395
CPC classification number: C07K16/065 , C07K1/22 , C07K16/2845 , C07K16/36 , C07K2317/24
Abstract: The present invention provides a method of purifying an antibody by protein A affinity chromatography. More specifically, the present invention provides a technique relating to an elution buffer solution which provides a good antibody recovery rate without denaturation.
Abstract translation: 本发明提供了通过蛋白A亲和层析纯化抗体的方法。 更具体地，本发明提供了一种提供良好抗体回收率而不变性的洗脱缓冲溶液的技术。
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公开(公告)号：US08002542B2
公开(公告)日：2011-08-23
申请号：US11822337
申请日：2007-07-05
Applicant: Daisuke Ejima , Tsuyoshi Kida
Inventor： Daisuke Ejima , Tsuyoshi Kida
IPC: F27D5/00
CPC classification number: F27D5/0037 , H01L21/673 , H01L24/97 , H01L2224/16 , H01L2224/16245 , H01L2224/45144 , H01L2224/48227 , H01L2224/48465 , H01L2224/73204 , H01L2224/73265 , H01L2924/00011 , H01L2924/00014 , H01L2924/181 , H01L2924/00 , H01L2224/0401
Abstract: A heat treatment jig includes a holding concave portion to hold an object and a void part formed near the holding concave portion and a thermal history variation of the object in a heat treatment process is reduced thereby.
Abstract translation: 热处理夹具包括用于保持物体的保持凹部和形成在保持凹部附近的空隙部，并且由此降低热处理工序中的物体的热历史变化。
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28.发明授权Method for improving recovery yield in protein purification with gel filtration chromatography 有权用凝胶过滤色谱法提高蛋白质纯化中的回收率的方法公开(公告)号：US07501495B2
公开(公告)日：2009-03-10
申请号：US11276509
申请日：2006-03-03
Applicant: Daisuke Ejima , Ryosuke Yumioka , Tsutomu Arakawa
Inventor： Daisuke Ejima , Ryosuke Yumioka , Tsutomu Arakawa
CPC classification number: C07K1/20 , C07K1/14 , C07K14/50 , C07K14/5412 , C07K14/55 , C07K14/575 , C07K14/755 , C07K16/36
Abstract: A method to more quantitatively recover a peak of a protein and associated aggregates, hydrophobic proteins, or hydrophobic peptides which are typically difficult to recover and detect as a peak, using a commercially available gel filtration chromatography column and a mobile phase is described. More specifically, a compound, such as arginine, which may be added to the mobile phase, thereby eliminating the unnecessary interaction that occurs between the proteins, peptides, or aggregates and the column stationary phase, is described.
Abstract translation: 描述了使用市售的凝胶过滤色谱柱和流动相来更定量地回收通常难以回收和检测作为峰的相关聚集体，疏水性蛋白质或疏水性肽的峰的方法。 更具体地，描述可以添加到流动相中的化合物，例如精氨酸，从而消除蛋白质，肽或聚集体和柱固定相之间发生的不必要的相互作用。
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公开(公告)号：US06756482B1
公开(公告)日：2004-06-29
申请号：US09721750
申请日：2000-11-27
Applicant: Kunio Ono , Shigekatsu Tsuchiya , Daisuke Ejima , Yuzuru Eto
Inventor： Kunio Ono , Shigekatsu Tsuchiya , Daisuke Ejima , Yuzuru Eto
IPC: A61K3824
CPC classification number: C07K14/575 , A61K38/00
Abstract: The present invention provides a process for purifying human activin by cation exchange chromatography and chaotropic ion concentration gradient elution.
Abstract translation: 本发明提供了通过阳离子交换色谱法和离液序列离子浓度梯度洗脱纯化人激活素的方法。
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