公开(公告)号：US11692220B2

公开(公告)日：2023-07-04

申请号：US17027433

申请日：2020-09-21

Applicant: Gen-Probe Incorporated

Inventor： David Buse ,  David Howard Combs ,  Norbert D. Hagen ,  David Opalsky ,  Bruce Richardson ,  Anita Prasad ,  Keith Moravick ,  Tyler Moore ,  Byron J. Knight

IPC: C12Q1/686 ,  B01L7/00 ,  G01N21/64 ,  B01L9/06

CPC classification number: C12Q1/686 ,  B01L7/52 ,  G01N21/6452 ,  B01L9/06 ,  B01L2200/141 ,  B01L2200/142 ,  B01L2300/042 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/1822 ,  G01N2021/6482 ,  G01N2021/6484

Abstract: An apparatus for performing nucleic acid amplification reactions includes a thermally-conductive receptacle holder with multiple receptacle wells. Each well has a through-hole extending from an inner surface of the well to an outer surface of the holder. A cover is rotatable between an open position and a closed position relative to the holder and is configured to exert a force onto any receptacles in the wells when the cover is in the closed position. The apparatus includes multiple optical fibers, and each of the optical fibers provides optical communication between one of the wells and an excitation signal source and/or an emission signal detector. A thermal element is positioned between a thermally-conductive support and the receptacle holder.

公开(公告)号：US11230733B2

公开(公告)日：2022-01-25

申请号：US17025735

申请日：2020-09-18

Applicant: Gen-Probe Incorporated

Inventor： David Buse ,  David Howard Combs ,  Norbert D. Hagen ,  David Opalsky ,  Bruce Richardson ,  Anita Prasad ,  Keith Moravick ,  Tyler Moore ,  Byron J. Knight

IPC: C12Q1/686 ,  B01L7/00 ,  G01N21/64 ,  B01L9/06

Abstract: Receptacles containing a reaction mixture are transported to receptacle wells of a thermally conductive receptacle holder, and the temperature of the holder is cycled with a thermoelectric device situated between a support and a first side of the holder while a first force is applied onto a second side of the holder. Optical communication between each well and an excitation signal source and an emission signal detector is established to determine whether an emission signal is emitted from any of the receptacles during temperature cycling as an indication of the presence of a target nucleic acid. The thermoelectric device may be situated between an upright portion of the support and the first side of the holder. A second force may be applied onto a top end of each receptacle in the holder by a cover moveable with respect to the receptacles between an open position and a closed position.

公开(公告)号：US11136617B2

公开(公告)日：2021-10-05

申请号：US17036307

申请日：2020-09-29

Applicant: Gen-Probe Incorporated

Inventor： Byron J. Knight ,  David A. Buse ,  Norbert D. Hagen ,  David Opalsky ,  Tyler Moore ,  Anita Prasad ,  Bruce Richardson

IPC: C12Q1/68 ,  B01L3/00 ,  C12Q1/6806 ,  G01N35/04 ,  B01L7/00 ,  C12Q1/686

Abstract: A cap that is securable to a vial includes a plug configured for insertion into an open end of the vial. An upper portion of the cap defines a probe recess with an open top end and configured to receive a distal end of a probe of a pipettor inserted into the open top end, to frictionally secure the cap to an end of the probe. The cap includes a radially-oriented annular surface with one or more locking members depending from a periphery of the annular surface. The locking members are spaced from the plug and each locking member includes a radial locking groove and a radial locking ridge beneath the radial locking groove. A lip of the vial snaps into the radial locking groove above the radial locking ridge to secure the cap to the vial when the plug is inserted into the open end of the vial.

公开(公告)号：US10889851B2

公开(公告)日：2021-01-12

申请号：US16829230

申请日：2020-03-25

Applicant: Gen-Probe Incorporated

Inventor： David A. Buse ,  Norbert D. Hagen ,  Byron J. Knight ,  Tyler Moore ,  David Opalsky ,  Anita Prasad ,  Bruce Richardson

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N35/04 ,  C12Q1/6806 ,  C12Q1/686

Abstract: A method for moving a processing vial between locations of an instrument. The method includes the steps of dispensing a fluid into a processing vial with a disposable pipette tip frictionally fitted onto a probe of a pipettor, stripping the disposable pipette tip from the probe of the pipettor, and then engaging a cap in a frictional fit with the probe of the pipettor. While the cap is engaged in a frictional fit with the probe of the pipettor, coupling the cap to the processing vial to form a cap/vial assembly, where the cap seals the processing vial. The pipettor moves the cap/vial assembly from a first location of an instrument to a second location of the instrument. At the second location of the instrument, the cap/vial assembly is ejected from the probe of the pipettor, thereby depositing the cap/vial assembly at the second location.