公开(公告)号：US10953399B2

公开(公告)日：2021-03-23

申请号：US16244776

申请日：2019-01-10

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Wesley A. Cox-Muranami ,  Darren Robert Segale ,  Oliver Jon Miller ,  Jennifer Olivia Foley ,  Tarun Kumar Khurana ,  Paul Crivelli ,  Gary Watts

IPC: G01N1/31 ,  B01L3/00 ,  B01L9/00

Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：US20240416351A1

公开(公告)日：2024-12-19

申请号：US18689691

申请日：2022-09-09

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli

IPC: B01L3/00

Abstract: Well assemblies and related methods are disclosed. In accordance with an implementation, an apparatus includes a well assembly including a body and an insert. The body has a well and the insert includes a sidewall defining an opening and a venting membrane coupled to the insert and extending across the opening. The insert is received within the well and wherein a coupling is formed between the sidewall of the insert and the body.

公开(公告)号：US12151240B2

公开(公告)日：2024-11-26

申请号：US18154563

申请日：2023-01-13

Applicant: ILLUMINA, INC.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: B01L3/00 ,  B01J19/00 ,  B01L9/00 ,  C12Q1/6837

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).

公开(公告)号：US12064761B2

公开(公告)日：2024-08-20

申请号：US16401805

申请日：2019-05-02

Applicant: Illumina, Inc.

Inventor： Cyril Delattre ,  Minsoung Rhee ,  Jeffrey Liu ,  Wesley Cox-Muranami ,  Paul Crivelli ,  Jennifer Foley ,  Darren Segale ,  Jay Taylor ,  Matthew Hage ,  Philip Paik ,  Erik Allegoren ,  David Hertzog ,  Alex Moroz-Smietana ,  Xiaoxiao Ma ,  Tsukasa Takahashi ,  Brandon Westerberg

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/527 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/16 ,  B01L2300/0887

Abstract: An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

公开(公告)号：US12000854B2

公开(公告)日：2024-06-04

申请号：US17831222

申请日：2022-06-02

Applicant: Illumina, Inc.

Inventor： Wesley A. Cox-Muranami ,  James Osmus ,  Paul Crivelli ,  Bradley Kent Drews

IPC: G01N35/10

CPC classification number: G01N35/1097

Abstract: An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.

公开(公告)号：US20230031325A1

公开(公告)日：2023-02-02

申请号：US17790058

申请日：2020-12-18

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Cyril Delattre ,  Gerald Kreindl ,  Wesley A. Cox-Muranami ,  Bryan Crane

IPC: B01L3/00

Abstract: Actuation systems and methods for use with flow cells. In accordance with an implementation, a method includes moving, using an actuator disposed within a manifold assembly, a membrane portion of a membrane of the manifold assembly away from a valve seat to enable fluidic flow from a reagent fluidic line to a common fluidic line. The membrane portion and the valve seat forming a membrane valve. The reagent fluidic line being fluidically coupled to a reagent reservoir. The common fluidic line being fluidically coupled to a flow cell. The common fluidic line has a common central axis and the reagent fluidic line has a reagent central axis that is non-collinear with the common central axis. The method includes urging the membrane portion against the valve seat to prevent fluidic flow from the reagent fluidic line to the common fluidic line.

公开(公告)号：US11376584B2

公开(公告)日：2022-07-05

申请号：US16400629

申请日：2019-05-01

Applicant: Illumina, Inc.

Inventor： Sz-Chin Lin ,  Jay Taylor ,  Minsoung Rhee ,  Jennifer Foley ,  Wesley Cox-Muranami ,  Cyril Delattre ,  Tarun Khurana ,  Paul Crivelli

IPC: G01N21/05 ,  G01N21/03 ,  B01L3/00

Abstract: In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.

公开(公告)号：US11192701B2

公开(公告)日：2021-12-07

申请号：US16510664

申请日：2019-07-12

Applicant: ILLUMINA, INC.

Inventor： Paul Crivelli ,  Gary Watts

IPC: B01L3/00 ,  B65D51/00 ,  B05C5/02

Abstract: A method is provided comprising loading shells into shell retention chambers of a shell management module, the shells including corresponding reservoirs configured to hold individual quantities of liquid, the shell retention chambers arranged in a predetermined pattern on a platform of the shell management module. The method further comprises orienting discharge ends of the shells along an actuation direction within the shell retention chambers, and covering the discharge ends with closure lids to seal bottoms of the corresponding reservoirs.

公开(公告)号：US20210197200A1

公开(公告)日：2021-07-01

申请号：US17115454

申请日：2020-12-08

Applicant: ILLUMINA, INC.

Inventor： Wesley A. Cox-Muranami ,  Cameron Mar ,  Cyril Delattre ,  Paul Crivelli ,  Kamal Shah ,  Kirkpatrick W. Norton

IPC: B01L3/00 ,  G01N27/327

Abstract: Systems for monitoring fluidics in reagent cartridges and related methods. An apparatus includes a system includes a reagent cartridge receptacle and a flow cell assembly. The apparatus includes a reagent cartridge receivable within the reagent cartridge receptacle and adapted to carry the flow cell assembly. The reagent cartridge includes a reagent reservoir fluidically coupled to the flow cell assembly. The apparatus includes a sensor module adapted to be positioned adjacent the reagent reservoir. The sensor module is adapted to generate a signal associated with a volume of reagent contained within the reagent reservoir.

公开(公告)号：US10518263B2

公开(公告)日：2019-12-31

申请号：US16264146

申请日：2019-01-31

Applicant: Illumina, Inc.

Inventor： Darren Segale ,  Hai Tran ,  Paul Crivelli

IPC: A61J1/06 ,  H01S4/00 ,  G01N35/00 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B01L3/00 ,  B01J19/00 ,  B01L9/00

Abstract: A circuit with electrical interconnect for external electronic connection and sensor(s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor(s). The laminated manifold includes fluidic channel(s), an interface between the die and the fluidic channel(s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel(s), attaching sensor(s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel(s).