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公开(公告)号:US20180100997A1
公开(公告)日:2018-04-12
申请号:US15838043
申请日:2017-12-11
Applicant: Leica Biosystems Imaging, Inc.
Inventor: Yunlu ZOU , Gregory CRANDALL , Allen OLSON
CPC classification number: G02B21/006 , G02B21/245 , G02B21/361 , G02B21/365
Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.
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公开(公告)号:US20170300617A1
公开(公告)日:2017-10-19
申请号:US15517489
申请日:2016-12-12
Applicant: LEICA BIOSYSTEMS IMAGING, INC.
Inventor: Walter GEORGESCU , Kiran SALIGRAMA , Allen OLSON , Bharat ANNALDAS
CPC classification number: G06F19/24 , G06K9/00 , G06K9/0014 , G06K9/62 , G06K9/80 , G06T7/11 , G06T7/62 , G06T2207/30024
Abstract: Automatic nuclear segmentation. In an embodiment, a plurality of superpixels are determined in a digital image. For each of the superpixels, any superpixels located within a search radius from the superpixel are identified, and, for each unique local combination between the superpixel and any identified superpixels located within the search radius from the superpixel, a local score for the local combination is determined. One of a plurality of global sets of local combinations with an optimum global score is identified based on the determined local scores.
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