公开(公告)号：US20050037511A1

公开(公告)日：2005-02-17

申请号：US10742459

申请日：2003-12-19

Applicant: Stephen Sharrock

Inventor： Stephen Sharrock

IPC: G01N21/25 ,  G01N21/27 ,  G01N21/47 ,  G01N21/78 ,  G01N21/86 ,  G01N21/00

CPC classification number: G01N21/8483 ,  G01N21/251 ,  G01N21/274 ,  G01N21/4738 ,  G01N21/78 ,  G01N2021/8488 ,  G01N2021/8494 ,  G01N2201/06106 ,  G01N2201/062

Abstract: An assay result reading device for reading the result of an assay performed using a liquid transport carrier may include at least one light source capable of emitting light incident upon at least one of two or more spatially separated zones of the carrier, a photodetector so positioned as to be capable of detecting light emanating from each of the two said zones and generating signals representing the presence or absence of a fluid sample in the respective zone, and a computation circuit. The computation circuit may be responsive to the signals to calculate a flow rate for a fluid flowing along the carrier, compare the calculated flow rate to upper and lower limits, and reject the assay result if the calculated flow rate is outside the upper and lower limits.

Abstract translation: 用于读取使用液体输送载体进行的分析结果的测定结果读取装置可以包括能够发射入射到载体的两个或更多个空间上分离的区域中的至少一个的光的至少一个光源， 能够检测从两个所述区域中的每一个发出的光并产生表示各个区域中是否存在流体样本的信号，以及计算电路。 计算电路可以响应于信号来计算沿着载体流动的流体的流量，将计算的流速与上限和下限进行比较，并且如果计算出的流量超出上限和下限，则拒绝测定结果 。

公开(公告)号：US5482842A

公开(公告)日：1996-01-09

申请号：US224054

申请日：1994-04-07

Applicant: Klaus W. Berndt

Inventor： Klaus W. Berndt

IPC: C12M1/34 ,  C12Q1/04 ,  G01N21/27 ,  G01N21/35 ,  G01N21/59 ,  G06Q50/00

CPC classification number: G01N21/274 ,  C12M41/46 ,  G01N2021/5969 ,  G01N21/3504 ,  G01N2201/06106 ,  G01N2201/066 ,  G01N2201/0662 ,  Y10S435/808

Abstract: Methods and apparatus for detecting biological activity within a sample are disclosed. The present invention provides a combination of a first and a second infrared light source arranged on the side of a sample vial, and a first and a second narrow-band infrared detector similarly arranged on the side of the vial approximately opposite the sources. The disclosed arrangement cancels the sources of error while measuring the carbon dioxide content of the headspace gas above the sample. In operation, the present invention sequentially measures the photocurrents generated at each detector with no source turned on, with the first source turned on, and with the second source turned on and the first source turned off. The CO.sub.2 absorption coefficient of the vial headspace gas is then calculated based on the photocurrents measured. This present invention allows compensation for source aging, detector aging, and vial wall thickness changes. Moreover, the present invention permits a determination of the absolute absorption coefficient at a selected wavelength, most preferably about 4.26 .mu.m, which is the CO.sub.2 absorption characteristic wavelength. The determination of the absolute CO.sub.2 concentration within the headspace permits the detection of bacterial growth processes. Additionally, the disclosed source/detector combination can be produced at low cost. Thus, in preferred embodiments, the apparatus of the present invention comprises a plurality of vials that are simultaneously monitored by providing each of the plurality of vials with its own source/detector combination and activating and deactivating the sources and detectors using a multiplexer/demultiplexer arrangement.

Abstract translation: 公开了用于检测样品内生物活性的方法和装置。 本发明提供了布置在样品瓶侧面的第一和第二红外光源的组合，以及类似地布置在大致与源相对的小瓶侧面上的第一和第二窄带红外检测器。 所公开的布置在测量样品上方的顶空气体的二氧化碳含量的同时消除误差源。 在操作中，本发明顺序地测量在每个检测器处产生的光电流，其中第一源被接通，并且第二源被接通，并且第一源被关断。 然后基于测量的光电流计算出瓶顶空气体的CO 2吸收系数。 本发明允许补偿源老化，检测器老化和小瓶壁厚变化。 此外，本发明允许确定选定波长的绝对吸收系数，最优选约4.26μm，这是CO 2吸收特性波长。 顶部空间中绝对二氧化碳浓度的测定允许细菌生长过程的检测。 另外，所公开的源/检测器组合可以以低成本生产。 因此，在优选实施例中，本发明的装置包括多个小瓶，其通过向多个小瓶中的每一个提供自己的源/检测器组合来同时监视，并使用多路复用器/解复用器布置来激活和去激活源和检测器 。