METHODS FOR APPLYING SMALL VOLUMES OF REAGENTS
    22.
    发明申请
    METHODS FOR APPLYING SMALL VOLUMES OF REAGENTS 失效
    适用于小量试剂的方法

    公开(公告)号:US20020081236A1

    公开(公告)日:2002-06-27

    申请号:US09437990

    申请日:1999-11-10

    Abstract: Methods, devices and apparatus are disclosed for carrying out multiple chemical reactions, such as in situ synthesis of polynucleotides, on a surface comprising an array of discrete sites. Molecules are deposited at a predetermined number of the discrete sites on the surface for reaction at the discrete sites. The surface is positioned relative to an outlet of a fluid ejection device, which is activated to dispense a small volume of a fluid through the outlet to the surface to provide uniform coating of a continuous region of the surface comprising a multiple of the discrete sites. The fluid is dispensed as uniform particles having a diameter such that the uniform particles form a sheet to coat the continuous region of the surface. In one embodiment of the present invention, liquid is dispensed as uniform particles through a fluid ejection device activated by means of ultrasonic energy. The invention has particular application to the in situ synthesis of polynucleotides in arrays on a surface.

    Abstract translation: 公开了用于在包括离散位置阵列的表面上进行多重化学反应的方法,装置和装置,例如原位合成多核苷酸。 分子沉积在表面上的预定数目的离散位置以在离散位置反应。 表面相对于流体喷射装置的出口定位,其被激活以将小体积的流体通过出口分配到表面,以提供包括多个离散位置的表面的连续区域的均匀涂覆。 流体被分配为具有使得均匀颗粒形成片以涂覆表面的连续区域的直径的均匀颗粒。 在本发明的一个实施例中,通过超声能量激活的流体喷射装置将液体作为均匀颗粒分配。 本发明特别适用于表面上阵列中多核苷酸的原位合成。

    Reactor distribution apparatus and quench zone mixing apparatus
    23.
    发明申请
    Reactor distribution apparatus and quench zone mixing apparatus 有权
    反应器分配装置和淬火区混合装置

    公开(公告)号:US20020039547A1

    公开(公告)日:2002-04-04

    申请号:US10000867

    申请日:2001-11-30

    Abstract: A quench zone mixing apparatus that occupies a low vertical height and has an improved mixing efficiency and fluid distribution across the catalyst surface includes a swirl chamber, a rough distribution network, and a distribution apparatus. In the swirl chamber, reactant fluid from a catalyst bed above is thoroughly mixed with a quench fluid by a swirling action. The mixed fluids exit the swirl chamber through an aperture to the rough distribution system where the fluids are radially distributed outward across the vessel to the distribution apparatus. The distribution apparatus includes a plate with a number of bubble caps and associated drip trays that multiply the liquid drip stream from the bubble caps to further symmetrically distribute the fluids across the catalyst surface. Alternatively, deflector baffles may be associated with the bubble caps to provide a wider and more uniform liquid distribution below the plate. The distribution apparatus can be used in the reaction vessel without the swirl chamber and rough distribution system, e.g., at the top of a vessel.

    Abstract translation: 占据低垂直高度并具有改善的混合效率和穿过催化剂表面的流体分布的骤冷区混合装置包括涡流室,粗分配网络和分配装置。 在涡流室中,来自上述催化剂床的反应物流体通过旋转作用与骤冷流体充分混合。 混合流体通过孔径离开涡流室至粗分配系统,其中流体沿着容器向外径向分布到分配装置。 分配设备包括具有多个气泡盖和相关的滴水盘的板,其将来自气泡盖的液体滴流加倍以进一步对称地分布流体穿过催化剂表面。 或者,偏转器挡板可以与泡罩相关联,以在板下方提供更宽和更均匀的液体分布。 分配装置可以在没有涡流室和粗分配系统的反应容器中使用,例如在容器的顶部。

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