公开(公告)号：US09952133B2

公开(公告)日：2018-04-24

申请号：US15599834

申请日：2017-05-19

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14 ,  G01N15/10

CPC classification number: G01N15/14 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2015/149

Abstract: A particle analyzer, comprising a source of a beam of pulsed optical energy; a detector comprising a number of spectral detection channels to detect optical signals resulting from interactions between the beam and particles in a sample (such as, e.g., fluorescence signals), and to convert the optical signals into respective electrical signals; optical paths from the source to the sample and from the sample to the detector; a flowcell connected with the optical paths and with a flow path for a suspension of particles; a signal processing module capable of: receiving the electrical signals from the detector; mathematically combining individual decay curves in the signals into a decay supercurve; allocating individual components of the supercurve to discrete bins of predetermined time constants; and quantifying the relative contribution of individual components to the supercurve; a particle sorting actuator; an actuator driver; and at least one particle collection receptacle.

公开(公告)号：US20170254738A1

公开(公告)日：2017-09-07

申请号：US15599834

申请日：2017-05-19

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14

CPC classification number: G01N15/14 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2015/149

Abstract: A particle analyzer, comprising a source of a beam of pulsed optical energy; a detector comprising a number of spectral detection channels to detect optical signals resulting from interactions between the beam and particles in a sample (such as, e.g., fluorescence signals), and to convert the optical signals into respective electrical signals; optical paths from the source to the sample and from the sample to the detector; a flowcell connected with the optical paths and with a flow path for a suspension of particles; a signal processing module capable of: receiving the electrical signals from the detector; mathematically combining individual decay curves in the signals into a decay supercurve; allocating individual components of the supercurve to discrete bins of predetermined time constants; and quantifying the relative contribution of individual components to the supercurve; a particle sorting actuator; an actuator driver; and at least one particle collection receptacle.

公开(公告)号：US20160103056A1

公开(公告)日：2016-04-14

申请号：US14879079

申请日：2015-10-08

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14

CPC classification number: G01N15/14 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2015/1488 ,  G01N2015/149

Abstract: A particle analyzer, comprising a source of a beam of pulsed optical energy; a detector comprising a number of spectral detection channels to detect optical signals resulting from interactions between the beam and particles in a sample (such as, e.g., fluorescence signals), and to convert the optical signals into respective electrical signals; optical paths from the source to the sample and from the sample to the detector; a flowcell connected with the optical paths and with a flow path for a suspension of particles; a signal processing module capable of: receiving the electrical signals from the detector; mathematically combining individual decay curves in the signals into a decay supercurve; allocating individual components of the supercurve to discrete bins of predetermined time constants; and quantifying the relative contribution of individual components to the supercurve; a particle sorting actuator; an actuator driver; and at least one particle collection receptacle.

Abstract translation: 一种粒子分析仪，包括脉冲光能束的源; 检测器，其包括多个光谱检测通道，以检测由样品中的光束和颗粒之间的相互作用（例如荧光信号）产生的光信号，并将光信号转换成相应的电信号; 从源到样品和从样品到检测器的光路; 与光路连接并具有用于悬浮颗粒的流动路径的流动池; 信号处理模块，其能够：从所述检测器接收电信号; 数学上将信号中的各个衰减曲线组合成衰变超曲线; 将超曲线的各个组件分配给具有预定时间常数的离散箱; 并量化各个组分对超曲的相对贡献; 粒子分选致动器; 执行器驱动器; 和至少一个颗粒收集容器。

公开(公告)号：US20240102914A1

公开(公告)日：2024-03-28

申请号：US18546508

申请日：2022-02-16

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Rick Yarussi ,  Elijah Sassoon Kashi

IPC: G01N15/14 ,  G01N15/1434

CPC classification number: G01N15/1459 ,  G01N15/1436 ,  G01N2015/1006

Abstract: This disclosure relates to an external monitoring device for a flow cytometer. The external monitoring device comprises: a housing configured to reversibly attach to a portion of the flow cytometer such that an optical system within the housing is aligned with a monitoring region within a flowcell of the flow cytometer. The optical system is configured to continuously capture and monitor at least a portion of the optical energy emanating from the monitoring region of the flowcell. The optical system comprises at least one sensor configured to: detect the optical energy captured by the optical system, and generate, based on the detected optical energy, an image of the monitoring region. The external monitoring device may further comprise a processor configured to continuously provide the image to an electronic device, for example a display or a computing device.

公开(公告)号：US11573167B2

公开(公告)日：2023-02-07

申请号：US17346893

申请日：2021-06-14

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14 ,  G01N15/10

Abstract: A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

公开(公告)号：US11543339B2

公开(公告)日：2023-01-03

申请号：US17308941

申请日：2021-05-05

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14 ,  G01N15/10

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US11536641B2

公开(公告)日：2022-12-27

申请号：US17307975

申请日：2021-05-04

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14 ,  G01N15/10

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20210341374A1

公开(公告)日：2021-11-04

申请号：US17328331

申请日：2021-05-24

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20210270720A1

公开(公告)日：2021-09-02

申请号：US17308941

申请日：2021-05-05

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20200278286A1

公开(公告)日：2020-09-03

申请号：US16876488

申请日：2020-05-18

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14

Abstract: A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.