公开(公告)号：US20240377357A1

公开(公告)日：2024-11-14

申请号：US18789966

申请日：2024-07-31

Applicant: University of Washington

Inventor： Jens H. Gundlach ,  Ian Michael Derrington ,  Andrew Laszlo ,  Jonathan Craig ,  Henry Brinkerhoff

IPC: G01N27/447 ,  G01N33/487 ,  G01N33/68

Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.

公开(公告)号：US20240376526A1

公开(公告)日：2024-11-14

申请号：US18784019

申请日：2024-07-25

Applicant: University of Washington

Inventor： Georg Seelig ,  Richard Muscat ,  Alexander B. Rosenberg

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

公开(公告)号：US20240376437A1

公开(公告)日：2024-11-14

申请号：US18444289

申请日：2024-02-16

Applicant: University of Washington

Inventor： Hongxia Fu ,  Benjamin Freedman ,  Jose A. Lopez

IPC: C12N5/071 ,  C12M3/00 ,  C12M3/06 ,  C12N9/22 ,  G01N33/50

Abstract: The present disclosure provides methods and compositions useful for generating cellular models of VWF disease from human pluripotent stem cells. In one aspect, the present disclosure relates to a method for the generation of an in vitro cellular model of VWF disease comprising VWF disease-relevant endothelial cells derived from human pluripotent stem cells (hPSCs). In an embodiment, the human pluripotent stem cells (hPSCs) are treated with an agent effective in suppressing or deleting at least one gene sequence encoding a protein and/or a subunit thereof, where the protein and/or subunit thereof is associated with VWF-linked secretion. The present disclosure also provides uses of the cellular models disclosed herein.

公开(公告)号：US20240369582A1

公开(公告)日：2024-11-07

申请号：US18557905

申请日：2022-05-20

Applicant: University of Washington

Inventor： Justin Chan ,  Shyamnath Gollakota ,  Kelly Michaelsen

IPC: G01N33/86 ,  B01L3/00

Abstract: Example systems may include an electronic device (e.g., a smartphone) and an attachment member that may couple the electronic device to a cup. The cup may be positioned within a field of view (e.g., within a focal length) of a camera that is coupled to or integral to the electronic device. The cup may receive a sample of whole blood and/or plasma. The attachment member may convey vibration from the electronic device to the cup. The cup may include a particle positioned in the cup. The camera may capture images of the cup including the particle, and motion of the particle may be used by the electronic device to calculate a clotting time of the sample. For example, the particle may move freely responsive to vibration during a time the sample is in a non-clotted state, but may slow and/or stop motion when the sample has clotted.

公开(公告)号：US20240368691A1

公开(公告)日：2024-11-07

申请号：US18651651

申请日：2024-04-30

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240368690A1

公开(公告)日：2024-11-07

申请号：US18651577

申请日：2024-04-30

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240367995A1

公开(公告)日：2024-11-07

申请号：US18681477

申请日：2022-08-09

Applicant: University of Washington

Inventor： Ding-Yuan KUO ,  Brandi M. COSSAIRT

IPC: C01G39/06 ,  B01D71/20 ,  C01G41/00 ,  H01M4/133 ,  H01M4/136 ,  H01M4/58 ,  H01M4/587

Abstract: Methods for making an intercalated layered film, intercalated layered films, and devices that include the intercalated layered films. In the method, in a first step, a suspension of a dispersed two-dimensional compound in a fluid is filtered through a filtration medium to provide a layered film of the two-dimensional compound on the filtration medium, and in a second step, filtering a solution of an intercalant in a solvent through the layered film of the two-dimensional compound on the filtration medium to provide the intercalated layered film.

公开(公告)号：US20240360502A1

公开(公告)日：2024-10-31

申请号：US18769519

申请日：2024-07-11

Applicant: University of Washington through its Center for Commercialization ,  Illumina, Inc.

Inventor： Jens H. Gundlach ,  Andrew Laszlo ,  Ian Derrington ,  Jeffrey G. Mandell

IPC: C12Q1/6869 ,  B01D57/02 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  B01D57/02 ,  G01N33/48721

Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：US20240337655A1

公开(公告)日：2024-10-10

申请号：US18597315

申请日：2024-03-06

Applicant: Trustees of Tufts College ,  University of Washington

Inventor： Fiorenzo G. Omenetto ,  Luciana d'Amone ,  Giuseppina Matzeu ,  Alfredo Quijano Rubio ,  David Baker

IPC: G01N33/566 ,  G01N33/569 ,  G01N33/573 ,  G01N33/74

CPC classification number: G01N33/566 ,  G01N33/56983 ,  G01N33/573 ,  G01N33/74 ,  G01N2333/43526

Abstract: A solid form sensing article can have one or more silk fibroin matrix sensing units, each having a solid form sensing property. Each of the one or more silk fibroin matrix sensing units can include a silk fibroin matrix. A plurality of Cage proteins and a plurality of Key proteins can be entrained within the silk fibroin matrix. The Cage proteins can bind to an analyte of interest.

公开(公告)号：US20240335515A1

公开(公告)日：2024-10-10

申请号：US18577620

申请日：2022-07-12

Applicant: University of Washington

Inventor： ELIZABETH NANCE ,  ANDREA JOSEPH ,  CHRIS NYAMBURA ,  WALTER JAMES PFAENDTNER ,  THOMAS WOOD

IPC: A61K38/44 ,  A61K47/54 ,  A61K47/61 ,  A61P25/28 ,  C12N9/08 ,  C12N11/08

CPC classification number: A61K38/44 ,  A61K47/541 ,  A61K47/61 ,  A61P25/28 ,  C12N9/0065 ,  C12N11/08 ,  C12Y111/01006

Abstract: Enzyme-loaded polymeric nanoparticles for the treatment of disorders such as neurological or non-neurological conditions are described. Using hydrophobic ion pairing, enzymes are loaded into polymeric nanoparticles while retaining enzymatic activity. Surfactants coating the nanoparticle can direct nanoparticles for cellular uptake or for aggregation in the extracellular matrix. Preparing a hydrophobic ion pairing complex for nanoparticle formation.