公开(公告)号：US09975965B2

公开(公告)日：2018-05-22

申请号：US15011543

申请日：2016-01-30

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Yi-Wei Lou ,  Chih-Wei Lin ,  Shih-Chi Yeh ,  Chung-Yi Wu ,  Han-Chung Wu

IPC: C07K16/44 ,  C07K16/18 ,  C07K16/30 ,  G01N33/574 ,  A61K39/00

CPC classification number: C07K16/44 ,  A61K2039/505 ,  C07K16/18 ,  C07K16/3053 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  G01N33/57407 ,  G01N2800/52

Abstract: Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to globo H, SSEA3, and SSEA-4 are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as those in brain, skin, bone, lungs, breast, esophagus, stomach, liver, bile duct, pancreas, colon, kidney, cervical, ovarian, and/or prostate cancer.

公开(公告)号：US09920347B2

公开(公告)日：2018-03-20

申请号：US15164968

申请日：2016-05-26

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Che Ma ,  Yung-Chieh Tseng

IPC: C12P21/00 ,  A61K39/12 ,  C07K14/005 ,  A61K39/145 ,  C12N7/00

CPC classification number: C12P21/005 ,  A61K39/12 ,  A61K39/145 ,  C07K14/005 ,  C12N7/00 ,  C12N2760/16111 ,  C12N2760/16121 ,  C12N2760/16123 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12Y302/01096 ,  G01N2333/00 ,  G01N2333/11 ,  Y02A50/386 ,  Y02A50/394 ,  Y02P20/52

Abstract: Methods for producing virus particles and viral antigens with simplified glycosylation profiles, such as mono-glycosylated forms of HIV, HCV, Dengue virus, West Nile virus and influenza virus. When used as targets for vaccine production, the conserved nature of such sites generate vaccines that are less sensitive to viral mutations. Such methods may include the use of glycosylation inhibitors for production of viruses or viral antigens with simplified glycosylation profiles.

公开(公告)号：US09874562B2

公开(公告)日：2018-01-23

申请号：US15210565

申请日：2016-07-14

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Jim-Min Fang ,  Yih-Shyun Edmund Cheng ,  Jiun-Jie Shie

IPC: G01N33/569

CPC classification number: G01N33/56983 ,  A61K31/665 ,  A61K31/683 ,  C07F9/6552 ,  G01N2333/11

Abstract: Methods and compositions for detection of drug resistant pathogens and treatment against infections thereof are provided. Methods for detection of oseltamivir-resistant influenza viruses by competitive binding assays utilizing non-oseltamivir influenza virus neuraminidase inhibitors and oseltamivir carboxylate are provided. Influenza virus neuraminidase inhibitors coupled to sensors and useful for employment in the methods of the invention are disclosed. Novel phosphonate compounds active as neuraminidase inhibitors against wild-type and oseltamivir-resistant influenza strains of H1N1, H5N1 and H3N2 viruses are disclosed. An enantioselective synthetic route to preparation of these phosphonate compounds via sialic acid is provided.

公开(公告)号：US09782476B2

公开(公告)日：2017-10-10

申请号：US14480521

申请日：2014-09-08

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: C07H15/18 ,  A61K39/39 ,  A61K31/7032 ,  A61K39/00

CPC classification number: A61K39/39 ,  A61K2039/55511 ,  A61K2039/57 ,  C07H15/18

Abstract: Glycosphingolipids (GSLs) bearing α-glucose (α-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with α-glucose (α-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with α-galactose (α-Gal) are disclosed. GSLs bearing α-glucose (α-Glc) and derivatives of α-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with α-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with α-Glc and derivatives thereof are provided.

公开(公告)号：US09340812B2

公开(公告)日：2016-05-17

申请号：US13971353

申请日：2013-08-20

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsung-I Tsai ,  Chung-Yi Wu

IPC: C12P19/18

CPC classification number: C12P19/18 ,  C12P19/04 ,  C12Y207/0701

Abstract: A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.

Abstract translation: 一种新型的UDP-Gal再生过程及其与半乳糖基转移酶的组合使用，以将半乳糖加入合适的受体底物。 本文还描述了大规模生产Globo系列寡糖的合成方法，其中所述方法可涉及糖基转移酶反应和核苷酸糖再生过程的组合。

公开(公告)号：US20150241439A1

公开(公告)日：2015-08-27

申请号：US14422939

申请日：2013-08-21

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Jim-Min Fang ,  Jiun-Jie Shie

IPC: G01N33/58 ,  C07D413/06 ,  C07D311/78

CPC classification number: G01N33/582 ,  A61K49/0032 ,  C07D311/78 ,  C07D311/94 ,  C07D405/06 ,  C07D405/14 ,  C07D413/06 ,  C07D491/04 ,  C07D493/04 ,  C07D495/04 ,  C07D513/04

Abstract: Provided are benzocyclooctyne compounds of formula (I). These compounds undergo strain-promoted azide-alkyne cyclo additions (SPAAC) without presence of toxic metal catalysts. The provided compounds are useful for diagnosis and imaging of azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed.

Abstract translation: 提供式（I）的苯并环辛烯化合物。 这些化合物在不存在有毒金属催化剂的情况下经历应变促进的叠氮炔 - 环加成（SPAAC）。 所提供的化合物可用于含叠氮化物分子的诊断和成像。 公开了使用本公开的化合物检测和成像生物分子的方法。

公开(公告)号：US20150225766A1

公开(公告)日：2015-08-13

申请号：US14422310

申请日：2013-08-16

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Jim-Min Fang ,  Yih-Shyun E. Cheng ,  Charng-Sheng Tsai

IPC: C12Q1/40 ,  C12Q1/70 ,  C07H5/00 ,  C12N9/24

CPC classification number: C12Q1/40 ,  C07H5/00 ,  C07H13/04 ,  C07H13/12 ,  C12N9/2402 ,  C12Q1/04 ,  C12Q1/34 ,  C12Q1/70 ,  C12Y302/01018 ,  G01N2333/11

Abstract: Provided herein are novel irreversible sialidase inhibitors. These compounds can be conjugated with a detectable tagging moiety such as azide-annexed biotin via CuAAC for isolation and identification of sialidases. The provided compounds and the corresponding detectable conjugates are useful for detecting sialidase-containing pathogens and imaging in situ sialidase activities under physiological conditions.

Abstract translation: 本文提供了新的不可逆唾液酸酶抑制剂。 这些化合物可以通过CuAAC与可检测的标记部分如叠氮化物附着的生物素缀合，用于分离和鉴定唾液酸酶。 提供的化合物和相应的可检测的缀合物可用于检测含唾液酸酶的病原体并在生理条件下成像原位唾液酸酶活性。

公开(公告)号：US20140051127A1

公开(公告)日：2014-02-20

申请号：US13971353

申请日：2013-08-20

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsung-I Tsai ,  Chung-Yi Wu

IPC: C12P19/18

CPC classification number: C12P19/18 ,  C12P19/04 ,  C12Y207/0701

Abstract: A novel UDP-Gal regeneration process and its combined use with a galactosyltransferase to add galactose to a suitable acceptor substrate. Also described herein are synthetic methods for generating Globo-series oligosaccharides in large scale, wherein the methods may involve the combination of a glycosyltransferase reaction and a nucleotide sugar regeneration process.

Abstract translation: 一种新型的UDP-Gal再生过程及其与半乳糖基转移酶的组合使用，以将半乳糖加入合适的受体底物。 本文还描述了大规模生产Globo系列寡糖的合成方法，其中所述方法可涉及糖基转移酶反应和核苷酸糖再生过程的组合。

公开(公告)号：US11919886B2

公开(公告)日：2024-03-05

申请号：US17811880

申请日：2022-07-12

Applicant: Academia Sinica ,  National Taiwan University

Inventor： Chi-Huey Wong ,  Pan-Chyr Yang ,  Rong-Jie Chein ,  Szu-Hua Pan ,  Ting-Jen R. Cheng

IPC: A61K31/4025 ,  A61K31/4178 ,  C07D307/92 ,  C07D403/12 ,  C07D405/12 ,  C07D413/12

CPC classification number: C07D405/12 ,  A61K31/4025 ,  A61K31/4178 ,  C07D307/92 ,  C07D403/12 ,  C07D413/12

Abstract: The present disclosure provides compounds of Formulas (I), (II), and pharmaceutically acceptable salts thereof. The compounds described herein are useful in treating proliferative diseases, for example, cancer (e.g., lung cancer), and infectious diseases (e.g., bacterial infections).

公开(公告)号：US11672853B2

公开(公告)日：2023-06-13

申请号：US16431099

申请日：2019-06-04

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Che Ma ,  Cheng-Chi Wang ,  Juine-Ruey Chen

IPC: A61K39/145 ,  C12N7/00 ,  A61K39/12 ,  C07K16/10 ,  A61K39/00

CPC classification number: A61K39/145 ,  A61K39/12 ,  C07K16/1018 ,  C12N7/00 ,  A61K2039/55505 ,  C07K2317/76 ,  C12N2740/11034 ,  C12N2740/16034 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16171 ,  C12N2770/24134 ,  Y02A50/30

Abstract: Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein E1 or E2 and HIV glycoproteins gp120 and gp41.