公开(公告)号：US10662402B2

公开(公告)日：2020-05-26

申请号：US15696176

申请日：2017-09-05

Applicant: FloDesign Sonics, Inc.

Inventor： Bart Lipkens ,  Erik Miller ,  Benjamin Ross-Johnsrud ,  Walter M. Presz, Jr. ,  Kedar Chitale ,  Thomas J. Kennedy, III ,  Lauryn Winiarski

IPC: B01D17/04 ,  C12M1/00 ,  B01D17/06 ,  B01D21/28 ,  B06B1/06 ,  C12M1/26 ,  C12M1/42 ,  C12N13/00 ,  H01L41/09

Abstract: Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

公开(公告)号：US10550382B2

公开(公告)日：2020-02-04

申请号：US15613790

申请日：2017-06-05

Applicant: FloDesign Sonics, Inc.

Inventor： Bart Lipkens ,  Walter M. Presz, Jr. ,  Kedar Chitale ,  Thomas J. Kennedy, III ,  Ben Ross-Johnsrud

IPC: C12N13/00 ,  B01D21/28 ,  A61M1/36 ,  C12M1/00 ,  G01N15/14 ,  B01L3/00 ,  G01N15/00 ,  G01N15/10

Abstract: Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.

公开(公告)号：US20190062690A1

公开(公告)日：2019-02-28

申请号：US15983823

申请日：2018-05-18

Applicant: FloDesign Sonics, Inc.

Inventor： Rui Tostoes ,  Bart Lipkens ,  Kedar Chitale ,  Benjamin Ross-Johnsrud ,  Walter M. Presz, JR. ,  Jack Saloio

IPC: C12M1/00 ,  B01D15/38 ,  C12M1/42 ,  C12N13/00

Abstract: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.

公开(公告)号：US09752113B2

公开(公告)日：2017-09-05

申请号：US15139187

申请日：2016-04-26

Applicant: FloDesign Sonics, Inc.

Inventor： Bart Lipkens ,  Erik Miller ,  Benjamin Ross-Johnsrud ,  Walter M. Presz, Jr. ,  Kedar Chitale ,  Thomas J. Kennedy, III ,  Lauren Winiarski

IPC: C12N13/00 ,  C12M1/00 ,  B01D17/04 ,  B01D17/06 ,  B01D21/28 ,  B06B1/06 ,  C12M1/26 ,  C12M1/42 ,  H01L41/09

CPC classification number: C12M47/02 ,  B01D17/04 ,  B01D17/06 ,  B01D21/283 ,  B06B1/0644 ,  C12M29/10 ,  C12M29/18 ,  C12M33/08 ,  C12M35/04 ,  C12N13/00 ,  H01L41/0973

Abstract: Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

公开(公告)号：USD787630S1

公开(公告)日：2017-05-23

申请号：US29541602

申请日：2015-10-06

Applicant: FloDesign Sonics, Inc.

Designer： Bart Lipkens ,  Jason Barnes ,  Dane Mealey ,  Walter M. Presz, Jr. ,  Brian McCarthy ,  Benjamin Ross-Johnsrud ,  Kedar Chitale

公开(公告)号：US20160319270A1

公开(公告)日：2016-11-03

申请号：US15143481

申请日：2016-04-29

Applicant: FloDesign Sonics, Inc.

Inventor： Bart Lipkens ,  Walter M. Presz, JR. ,  Kedar Chitale ,  Thomas J. Kennedy, III

IPC: C12N13/00 ,  C12M1/00

CPC classification number: C12N13/00 ,  A61M1/3678 ,  B01D21/283 ,  B01L3/502761 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0436 ,  B01L2400/0439 ,  C12M47/04 ,  G01N15/1484 ,  G01N2015/0053 ,  G01N2015/1006 ,  G01N2015/149

Abstract: Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.

Abstract translation: 公开了用于从主体流体分离材料的装置。 这些装置包括流动室，超声波换能器和反射器。 超声换能器和反射器产生相对于通过流动室的平均流动方向以一定角度定向的倾斜声驻波。 倾斜的声驻波导致具有使材料偏转的轴向力分量的声辐射力，从而可以分离材料和主体流体。 倾斜的声驻波可以相对于通过流动室的平均流动的方向以大约20°至大约70°的角度定向，从而偏离，收集，分离或分流材料与流过流过装置的流体 速率约400mL / min，高达约700mL / min。

公开(公告)号：US20160279540A1

公开(公告)日：2016-09-29

申请号：US15080354

申请日：2016-03-24

Applicant: FloDesign Sonics, Inc.

Inventor： Walter M. Presz, JR. ,  Kedar Chitale ,  Bart Lipkens

IPC: B01D21/28 ,  C12M1/00 ,  C02F1/36

CPC classification number: B01D21/283 ,  C02F1/36 ,  C12M47/02

Abstract: Devices for separating materials from a host fluid are disclosed. The devices include an acoustic chamber having an inlet and an outlet. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps the materials and permits a continuous separation of the materials from the host fluid. The materials and the host fluid can thus be separately collected. Multiple sets of trapping lines are generated by the acoustic standing wave, and the transducer is oriented to minimize cross-sectional area for straight vertical channels between the trapping lines.

Abstract translation: 公开了用于从主体流体分离材料的装置。 这些装置包括具有入口和出口的声学室。 超声波换能器和反射器在声学室中产生多维声驻波，捕获材料并允许材料与主体流体的连续分离。 因此，可以分别收集材料和主体流体。 通过声驻波产生多组捕获线，并且换能器被定向成使捕获线之间的直立垂直通道的横截面面积最小化。

公开(公告)号：US20160237394A1

公开(公告)日：2016-08-18

申请号：US15139187

申请日：2016-04-26

Applicant: FloDesign Sonics, Inc.

Inventor： Bart Lipkens ,  Erik Miller ,  Benjamin Ross-Johnsrud ,  Walter M. Presz, JR. ,  Kedar Chitale ,  Thomas J. Kennedy, III

IPC: C12M1/00 ,  C12N13/00

CPC classification number: C12M47/02 ,  B01D17/04 ,  B01D17/06 ,  B01D21/283 ,  B06B1/0644 ,  C12M29/10 ,  C12M29/18 ,  C12M33/08 ,  C12M35/04 ,  C12N13/00 ,  H01L41/0973

Abstract: Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.

Abstract translation: 公开了用于将流体混合物中的生物细胞与其它材料分离的声学灌注装置。 这些装置包括连接到声学室的入口端口，出口端口和收集端口。 超声波换能器在声学室中产生声驻波，其允许通过收集口回收的流体的连续流动，同时保持声室内的生物细胞返回到生物反应器，流体混合物从该反应器被拉出。