公开(公告)号：US11573165B2

公开(公告)日：2023-02-07

申请号：US17326662

申请日：2021-05-21

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14 ,  G01N15/10

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US10656072B2

公开(公告)日：2020-05-19

申请号：US16154642

申请日：2018-10-08

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14 ,  G01N15/10

Abstract: A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

公开(公告)号：US09778193B2

公开(公告)日：2017-10-03

申请号：US14461293

申请日：2014-08-15

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/00 ,  G01N21/65 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N21/65 ,  G01N15/1434 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2021/653 ,  G01N2201/06113 ,  G01N2201/0697

Abstract: An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles.

公开(公告)号：US20240102914A1

公开(公告)日：2024-03-28

申请号：US18546508

申请日：2022-02-16

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Rick Yarussi ,  Elijah Sassoon Kashi

IPC: G01N15/14 ,  G01N15/1434

CPC classification number: G01N15/1459 ,  G01N15/1436 ,  G01N2015/1006

Abstract: This disclosure relates to an external monitoring device for a flow cytometer. The external monitoring device comprises: a housing configured to reversibly attach to a portion of the flow cytometer such that an optical system within the housing is aligned with a monitoring region within a flowcell of the flow cytometer. The optical system is configured to continuously capture and monitor at least a portion of the optical energy emanating from the monitoring region of the flowcell. The optical system comprises at least one sensor configured to: detect the optical energy captured by the optical system, and generate, based on the detected optical energy, an image of the monitoring region. The external monitoring device may further comprise a processor configured to continuously provide the image to an electronic device, for example a display or a computing device.

公开(公告)号：US11573167B2

公开(公告)日：2023-02-07

申请号：US17346893

申请日：2021-06-14

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14 ,  G01N15/10

Abstract: A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

公开(公告)号：US11543339B2

公开(公告)日：2023-01-03

申请号：US17308941

申请日：2021-05-05

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14 ,  G01N15/10

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US11536641B2

公开(公告)日：2022-12-27

申请号：US17307975

申请日：2021-05-04

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14 ,  G01N15/10

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20210341374A1

公开(公告)日：2021-11-04

申请号：US17328331

申请日：2021-05-24

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20210270720A1

公开(公告)日：2021-09-02

申请号：US17308941

申请日：2021-05-05

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca ,  Ralph Jimenez

IPC: G01N15/14

Abstract: Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

公开(公告)号：US20200278286A1

公开(公告)日：2020-09-03

申请号：US16876488

申请日：2020-05-18

Applicant: Kinetic River Corp.

Inventor： Giacomo Vacca

IPC: G01N15/14

Abstract: A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a specific cross-sectional aspect ratio; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.