公开(公告)号：US4794450A

公开(公告)日：1988-12-27

申请号：US90477

申请日：1987-08-28

Applicant: Tomo Saito ,  Yasusuke Sakurabayashi ,  Toshitsugu Inouchi ,  Norihiro Suzuki

Inventor： Tomo Saito ,  Yasusuke Sakurabayashi ,  Toshitsugu Inouchi ,  Norihiro Suzuki

IPC: G01N21/59 ,  G01N21/82 ,  G01N33/53 ,  H04N7/18 ,  G06K9/00

CPC classification number: G01N21/82 ,  G01N33/53 ,  G01N2021/5961 ,  G01N2201/0461

Abstract: An apparatus for judging an agglutination has a transparent plate stand 5 for supporting a microplate 7 and perforated with positioning openings 9 for centering of each well 8 of the microplate. A light source and TV camera 12 are disposed above the microplate, an image memory 13 stores the output of the camera, a processor 15 differentiates the stored image to determine its profile, and the picture elements of the differentiated image having intensity levels larger than zero are counted. A calculator 17 produces coefficients of variation, which are plotted against the image profile and compared with a reference value to remove contrast irregularities.

Abstract translation: 用于判断凝集的装置具有用于支撑微板7的透明板支架5，并且穿过定位开口9，用于定位微孔板的每个孔8。 光源和电视摄像机12设置在微板上方，图像存储器13存储照相机的输出，处理器15区分所存储的图像以确定其轮廓，并且具有大于零的强度水平的差分图像的像素 算了 计算器17产生变化系数，其相对于图像轮廓绘制并与参考值进行比较以去除对比度不规则。

公开(公告)号：US4554257A

公开(公告)日：1985-11-19

申请号：US489734

申请日：1983-04-29

Applicant: Frederick J. Aladjem ,  Padmasini K. Ayengar

Inventor： Frederick J. Aladjem ,  Padmasini K. Ayengar

IPC: A61K39/00 ,  G01N15/10 ,  G01N21/82 ,  G01N33/543 ,  G01N35/00

CPC classification number: G01N33/54313 ,  G01N15/10 ,  G01N21/82 ,  G01N2201/0446 ,  G01N2201/0461 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/827

Abstract: The disclosed methods improve conventional agglutination processes for assaying immunoreactive and like substances, primarily by improving the measurement of the agglutination itself. Suspensions containing agglutinated particles are automatically inspected, preferably intermittently during the agglutinating reaction, and the resulting data are processed to identify individual particle aggregates of a selected limited class, which may, for example, comprise aggregates having sizes within a limited size interval. The numbers of such aggregates are compared with corresponding reference values obtained with standard solutions and suitable controls to evaluate the concentration of one of the reactive substances, or other information. The aggregate size intervals and other parameters which are used to define aggregate classes are preferably selected with attention to the detailed behavior of each test system.

Abstract translation: 所公开的方法改进了用于测定免疫反应性和类似物质的常规凝集方法，主要通过改进凝集本身的测量。 自动检查含有凝集颗粒的悬浮液，优选在凝集反应期间间歇地进行检查，并且对所得数据进行处理以鉴定所选择的有限类别的单个颗粒聚集体，其可以例如包含尺寸在有限尺寸间隔内的聚集体。 将这些聚集体的数量与用标准溶液和合适的对照获得的相应参考值进行比较，以评估其中一种反应物质或其它信息的浓度。 用于定义聚合类的聚合大小间隔和其他参数优选地被选择，注意每个测试系统的详细行为。