公开(公告)号：US20050079510A1

公开(公告)日：2005-04-14

申请号：US10767899

申请日：2004-01-28

Applicant: Jan Berka ,  Yi-Ju Chen ,  John Leamon ,  Steve Lefkowitz ,  Kenton Lohman ,  Vinod Makhijani ,  Jonathan Rothberg ,  Gary Sarkis ,  Maithreyan Srinivasan ,  Michael Weiner

Inventor： Jan Berka ,  Yi-Ju Chen ,  John Leamon ,  Steve Lefkowitz ,  Kenton Lohman ,  Vinod Makhijani ,  Jonathan Rothberg ,  Gary Sarkis ,  Maithreyan Srinivasan ,  Michael Weiner

IPC: B01L3/00 ,  C07H21/00 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/25 ,  G01N21/64

CPC classification number: C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Abstract translation: 公开了用于核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化，并扩增核酸模板以提供附着于珠粒上的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。

公开(公告)号：US20050037375A1

公开(公告)日：2005-02-17

申请号：US10748805

申请日：2003-12-29

Applicant: Vimal Mehta ,  Thomas Jarvie ,  Jonathan Rothberg

Inventor： Vimal Mehta ,  Thomas Jarvie ,  Jonathan Rothberg

IPC: G01N33/53 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/542 ,  G01N33/566 ,  G01N33/68 ,  G01N33/567

CPC classification number: G01N33/542 ,  C12Q1/6897

Abstract: A rapid method and kit is provided for identifying the targets of biologically active small molecules to identify new drugs that are capable of specific therapeutic effects as well as to identify novel small molecules including agonists and antagonists that may bind selected targets.

Abstract translation: 提供了一种快速的方法和试剂盒，用于鉴定生物活性小分子的靶标，以鉴定能够具有特定治疗作用的新药物，以及鉴定可能结合所选靶标的新型小分子，包括激动剂和拮抗剂。

公开(公告)号：US08540868B2

公开(公告)日：2013-09-24

申请号：US13229456

申请日：2011-09-09

Applicant: Jonathan Rothberg ,  Wolfgang Hinz

Inventor： Jonathan Rothberg ,  Wolfgang Hinz

IPC: G01N27/414 ,  G01N33/50

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US08540866B2

公开(公告)日：2013-09-24

申请号：US13229402

申请日：2011-09-09

Applicant: Jonathan Rothberg ,  Wolfgang Hinz

Inventor： Jonathan Rothberg ,  Wolfgang Hinz

IPC: G01N27/414 ,  G01N33/50

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US08540865B2

公开(公告)日：2013-09-24

申请号：US13228058

申请日：2011-09-08

Applicant: Jonathan Rothberg ,  Wolfgang Hinz

Inventor： Jonathan Rothberg ,  Wolfgang Hinz

IPC: G01N27/414 ,  G01N33/50

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US08535513B2

公开(公告)日：2013-09-17

申请号：US13616454

申请日：2012-09-14

Applicant: Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: C12Q1/25 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US08496802B2

公开(公告)日：2013-07-30

申请号：US13555998

申请日：2012-07-23

Applicant: Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: G01N27/403

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测氢离子浓度（pH），其他分析物浓度变化和/或与DNA合成相关的化学过程相关联的结合事件的变化来促进DNA测序技术。

公开(公告)号：US20080003142A1

公开(公告)日：2008-01-03

申请号：US11803104

申请日：2007-05-11

Applicant: Darren Link ,  Michael Weiner ,  David Marran ,  Jonathan Rothberg

Inventor： Darren Link ,  Michael Weiner ,  David Marran ,  Jonathan Rothberg

IPC: G01N21/64 ,  B01L3/00 ,  B29C33/38

CPC classification number: B01F3/0807 ,  B01F13/0062 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502784 ,  B01L3/565 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1068 ,  C12N15/1086 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N15/147 ,  G01N21/05 ,  G01N21/64 ,  G01N27/3275 ,  G01N35/08 ,  G01N2021/0346 ,  G01N2035/00237 ,  G01N2035/00326 ,  G01N2201/024 ,  Y10T137/87571 ,  Y10T137/87619 ,  Y10T137/87652 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2531/113

Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

Abstract translation: 本发明提供了可用于进行生物学，化学和诊断测定的新型微流体底物和方法。 基板可以包括多个可电寻址的通道支承流体模块，其一体地布置成使得提供用于不混溶流体流动的连续通道。

公开(公告)号：US20070042421A1

公开(公告)日：2007-02-22

申请号：US11592067

申请日：2006-11-01

Applicant: Jonathan Rothberg ,  Michael McKenna ,  Paul Predki ,  Andreas Windemuth ,  Richard Shimkets

Inventor： Jonathan Rothberg ,  Michael McKenna ,  Paul Predki ,  Andreas Windemuth ,  Richard Shimkets

IPC: C40B40/08 ,  C40B30/06

CPC classification number: C12N15/1096 ,  C12N15/1034 ,  C12N15/1093

Abstract: Disclosed are methods for identifying nucleic acids in a sample of nucleic acids in which nucleic acids are initially present in unequal amounts. The methods include partitioning the starting population of nucleic acids to form one or more subpopulations, and then identifying nucleic acids that are present in different amounts in the partitioned nucleic acid sample as compared to the starting population.

Abstract translation: 公开了用于鉴定核酸样品中核酸最初以不等量存在的方法。 所述方法包括分配核酸的起始群体以形成一个或多个亚群体，然后鉴定与起始群体相比在分配的核酸样品中以不同的量存在的核酸。

公开(公告)号：US20060160178A1

公开(公告)日：2006-07-20

申请号：US10991309

申请日：2004-11-17

Applicant: Jonathan Rothberg ,  Michael Weiner

Inventor： Jonathan Rothberg ,  Michael Weiner

IPC: C12P21/06 ,  C07K16/18 ,  C12N1/21 ,  C07H21/04 ,  C12N5/06 ,  C12N1/18 ,  C12N15/74

CPC classification number: C07K16/2803 ,  C07K16/241 ,  C07K2317/34 ,  C07K2317/622

Abstract: The present invention provides a high throughput method for generating fully human monoclonal antibodies.

Abstract translation: 本发明提供了用于产生完全人单克隆抗体的高通量方法。