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公开(公告)号：US20070178556A1
公开(公告)日：2007-08-02
申请号：US11712336
申请日：2007-02-28
Applicant: Robert DiCosimo , Mark Payne , Anna Panova , Jeffery Thompson , Daniel O'Keefe
Inventor： Robert DiCosimo , Mark Payne , Anna Panova , Jeffery Thompson , Daniel O'Keefe
Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.
Abstract translation: 提供了用于从乙醇腈生产乙醇酸的各种方法。 这些方法包括：1）使用具有改进的腈水解酶活性的乙酰甘氨酸转化成乙醇酸的Acidovorax facilis 72W腈水解酶突变体，和2）提高催化剂稳定性和/或生产率的方法。 提高催化剂稳定性/生产率的方法包括使用反应稳定剂，在基本上无氧条件下进行反应，以及控制反应混合物中底物的浓度。
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42.发明申请Nucleic acid fragments encoding nitrile hydratase and amidase enzymes from comamonas testosteroni 5-MGAM-4D and recombinant organisms expressing those enzymes useful for the production of amides and acids 失效编码来自彗星彗星5-MGAM-4D的腈水合酶和酰胺酶的核酸片段和表达可用于产生酰胺和酸的那些酶的重组生物公开(公告)号：US20070154997A1
公开(公告)日：2007-07-05
申请号：US11601156
申请日：2006-11-16
Applicant: Mark Payne , Robert DiCosimo , John Gavagan , Robert Fallon
Inventor： Mark Payne , Robert DiCosimo , John Gavagan , Robert Fallon
CPC classification number: C12N9/88 , C07K14/195 , C12P13/02 , C12P17/12 , C12P21/02
Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.
Abstract translation: 本发明涉及编码来自Comamonas睾丸酮5-MGAM-4D的腈水合酶（NHase）或酰胺酶（Am）的基因的分离，测序和重组表达，其中NH酶可用于催化腈水合至相应的 酰胺，并且酰胺酶可用于将酰胺水解成相应的羧酸。 还提供了转化宿主细胞，其含有用于从睾丸酮睾酮5-MGAM-4D中表达腈水合酶或酰胺酶的多核苷酸。
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公开(公告)号：US20070105740A1
公开(公告)日：2007-05-10
申请号：US11413246
申请日：2006-04-28
Applicant: Robert Dicosimo , Mark Payne , Vincent Croud , John Gavagan , Lorraine Wagner , Eugenia Hann
Inventor： Robert Dicosimo , Mark Payne , Vincent Croud , John Gavagan , Lorraine Wagner , Eugenia Hann
CPC classification number: A61L2/186 , A61L2/183 , A61L2202/18 , A61L2202/24 , A61L2202/26 , C11D1/521 , C11D3/2093 , C11D3/32 , C11D3/386 , C11D3/3947 , C11D3/48 , C12P7/00 , C12P7/40
Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.
Abstract translation: 提供了一种方法，在中性至酸性反应条件下，使用至少一种在过氧化氢（至少500mM浓度）存在下具有过水解酶活性的酶，从合适的羧酸酯（包括 甘油酯）和/或酰胺底物。 所生产的浓缩的过酸溶液足以用于各种消毒和/或漂白应用。
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44.发明授权Nucleic acid fragments encoding nitrile hydratase and amidase enzymes from Comamonas testosteroni 5-MGAM-4D and recombinant organisms expressing those enzymes useful for the production of amides and acids 失效从Comamonas testosteroni 5-MGAM-4D编码腈水合酶和酰胺酶的核酸片段和表达可用于产生酰胺和酸的酶的重组生物公开(公告)号：US07153663B2
公开(公告)日：2006-12-26
申请号：US10977893
申请日：2004-10-29
Applicant: Mark S. Payne , Robert DiCosimo , John E. Gavagan , Robert D. Fallon
Inventor： Mark S. Payne , Robert DiCosimo , John E. Gavagan , Robert D. Fallon
CPC classification number: C12N9/88 , C07K14/195 , C12P13/02 , C12P17/12 , C12P21/02
Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.
Abstract translation: 本发明涉及编码来自Comamonas睾丸酮5-MGAM-4D的腈水合酶（NHase）或酰胺酶（Am）的基因的分离，测序和重组表达，其中NH酶可用于催化腈水合至相应的 酰胺，并且酰胺酶可用于将酰胺水解成相应的羧酸。 还提供了转化宿主细胞，其含有用于从睾丸酮睾酮5-MGAM-4D中表达腈水合酶或酰胺酶的多核苷酸。
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公开(公告)号：US07138480B2
公开(公告)日：2006-11-21
申请号：US10378201
申请日：2003-03-03
Applicant: Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
Inventor： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
IPC: C08G63/06
Abstract: This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.
Abstract translation: 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 3-羟基戊酸用于制备高支化共聚酯中的ε-己内酯的替代物。
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Patent Agency Ranking
46.发明授权Isolation and expression of a gene for a nitrilase from Acidovorax faclilis 72W 失效从酸性酵母72W中分离和表达腈水解酶基因公开(公告)号：US07056709B2
公开(公告)日：2006-06-06
申请号：US10376653
申请日：2003-02-27
Applicant: Sarita Chauhan , Robert Dicosimo , Robert D. Fallon , John E. Gavagan , Mark S. Payne
Inventor： Sarita Chauhan , Robert Dicosimo , Robert D. Fallon , John E. Gavagan , Mark S. Payne
IPC: C12P7/40 , C07H21/04 , C12Q1/68 , C07D211/60
CPC classification number: C12P13/00 , C12N9/78 , C12P7/40 , C12P13/002 , C12P13/02
Abstract: Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
Abstract translation: 提供重组微生物菌株，其表达腈水解酶，并且可用作用于含腈底物水解的生物催化剂。 将重组细胞用分离自Acidovorax facilis 72W的外源基因转化，其编码热稳定的腈水解酶，其在温和的反应条件下催化含腈底物水解成羧酸。 提供了腈水解酶基因的核苷酸序列和由腈水解酶基因编码的推导的氨基酸序列。
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47.发明授权Continuous bioreactor process for the preparation of polyester cyclic oligomers 失效用于制备聚酯环状低聚物的连续生物反应器工艺公开(公告)号：US07026433B2
公开(公告)日：2006-04-11
申请号：US10698275
申请日：2003-10-31
Applicant: Edward G. Brugel , Robert DiCosimo
Inventor： Edward G. Brugel , Robert DiCosimo
Abstract: A continuous process for the process for the enzyme-catalyzed preparation of cyclic ester oligomers from linear ester oligomers. The process may use a linear or recirculating reactor.
Abstract translation: 用于从线性酯低聚物酶催化制备环状酯低聚物的方法的连续方法。 该方法可以使用线性或循环反应器。
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48.发明授权Isolation and expression of a gene for a nitrilase from Acidovorax facilis 72W 有权从Acidovorax facilis 72W中分离和表达腈水解酶基因公开(公告)号：US06870038B2
公开(公告)日：2005-03-22
申请号：US09823373
申请日：2001-03-30
Applicant: Sarita Chauhan , Robert Dicosimo , Robert D. Fallon , John E. Gavagan , Mark S. Payne
Inventor： Sarita Chauhan , Robert Dicosimo , Robert D. Fallon , John E. Gavagan , Mark S. Payne
IPC: C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/78 , C12P7/40 , C12P13/00 , C12P17/10 , C12R1/19 , C07H21/02 , C07H21/04 , C12Q1/68
CPC classification number: C12P13/00 , C12N9/78 , C12P7/40 , C12P13/002 , C12P13/02
Abstract: Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
Abstract translation: 提供重组微生物菌株，其表达腈水解酶，并且可用作用于含腈底物水解的生物催化剂。 将重组细胞用分离自Acidovorax facilis 72W的外源基因转化，其编码热稳定的腈水解酶，其在温和的反应条件下催化含腈底物水解成羧酸。 提供了腈水解酶基因的核苷酸序列和由腈水解酶基因编码的推导的氨基酸序列。
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49.发明授权Method for stabilizing nitrilase activity and preserving microbial cells with carbamate salts 有权稳定腈水解酶活性和用氨基甲酸盐保存微生物细胞的方法公开(公告)号：US06677149B2
公开(公告)日：2004-01-13
申请号：US09854498
申请日：2001-05-14
Applicant: Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
Inventor： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
IPC: C12N104
CPC classification number: C12N1/04 , C12N9/78 , C12N9/96 , C12N11/04 , C12N11/08 , C12N11/10 , C12P13/00
Abstract: A method for preserving immobilized or unimmobilized microbial cells having nitrilase activity and for stabilizing the nitrilase activity of unimmobilized or immobilized microbial cells has been developed. Aqueous suspensions containing at least 100 mM bicarbonate, carbonate, or carbamate salts limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), Acidovorax facilis 72W (ATCC 55746), and transformed microbial cells having nitrilase activity, the host cells transformed with Acidovorax facilis 72W nitrilase activity. Especially preferred is an embodiment using ammonium carbamate as the inorganic salt.
Abstract translation: 已经开发了用于保存具有腈水解酶活性的固定或未固化的微生物细胞并稳定未固定或固定的微生物细胞的腈水解酶活性的方法。 含有至少100mM碳酸氢盐，碳酸盐或氨基甲酸盐的含水悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固化或固定的细胞的所需腈水解酶活性。 其特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745），Acidovorax facilis 72W（ATCC 55746）和 转化的具有腈水解酶活性的微生物细胞，宿主细胞用Acidovorax facilis 72W腈水解酶活性转化。 特别优选的是使用氨基甲酸铵作为无机盐的实施方案。
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公开(公告)号：US5219745A
公开(公告)日：1993-06-15
申请号：US705419
申请日：1991-05-24
Applicant: David L. Anton , Robert DiCosimo , Lawrence W. Gosser
Inventor： David L. Anton , Robert DiCosimo , Lawrence W. Gosser
IPC: C12P7/40
CPC classification number: C12P7/40
Abstract: A process for the production of glyoxylic acid involving the enzymatic oxidation of glycolic acid. The process provides a commercially practical method involving the reaction of glycolic acid in an aqueous solution at a starting concentration range of 200 mM to 2,500 mM in the presence of oxygen, glycolate oxidase and catalase at a pH of 7 to 10 and in the presence of an amine such as ethylenediamine, or tris(hydroxymethyl)-methylamine.
Abstract translation: 涉及乙醇酸酶促氧化的乙醛酸的生产方法。 该方法提供了一种商业上实用的方法，其涉及在氧气，乙醇酸氧化酶和过氧化氢酶的存在下，在pH为7至10的条件下，以200mM至2,500mM的起始浓度范围的乙醇酸反应， 胺如乙二胺或三（羟甲基） - 甲基胺。
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