公开(公告)号：US11638705B2

公开(公告)日：2023-05-02

申请号：US16831346

申请日：2020-03-26

Applicant: Academia Sinica

Inventor： Rong-Jie Chein ,  Pan-Chyr Yang ,  Chi-Huey Wong ,  Ming-Shiu Lin ,  Ting-Jen Cheng ,  Ting-Hung Chou

IPC: A61K31/47 ,  A61P35/00 ,  A61K45/06 ,  C07D215/233

Abstract: Compounds of Formula (I) and methods for treating, delaying, and/or preventing the adverse effects of proliferative diseases, such as cancers including, for example, lung cancer, breast cancer, ovarian cancer, prostate cancer, head cancer, neck cancer, head and neck cancer, or leukemia (e.g., cancer resistant to treatment by one or more microtubule-targeting agents, e.g., cancer resistant to multiple drugs associated with P-glycoprotein (P-gp) overexpression). Methods of inhibiting polymerization of a cancer cell microtubule in a subject in need thereof or a cell, tissue, or biological sample, binding β-tubulin, inhibiting microtubule assembly and, inducing apoptosis in a cancer cell resistant to multiple drugs in a tissue, biological sample, or subject. Pharmaceutical compositions, kits, and methods of using the compounds for treating any of the target diseases described herein.

公开(公告)号：US20230010562A1

公开(公告)日：2023-01-12

申请号：US17811880

申请日：2022-07-12

Applicant: Academia Sinica ,  National Taiwan University

Inventor： Chi-Huey Wong ,  Pan-Chyr Yang ,  Rong-Jie Chein ,  Szu-Hua Pan ,  Ting-Jen R. Cheng

IPC: C07D405/12 ,  C07D307/92 ,  C07D403/12 ,  C07D413/12

Abstract: The present disclosure provides compounds of Formulas (I), (II), and pharmaceutically acceptable salts thereof. The compounds described herein are useful in treating proliferative diseases, for example, cancer (e.g., lung cancer), and infectious diseases (e.g., bacterial infections).

公开(公告)号：US11332523B2

公开(公告)日：2022-05-17

申请号：US14722775

申请日：2015-05-27

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Chung-Yi Wu

IPC: C07K16/24 ,  A61K39/395 ,  A61K45/06 ,  C12P21/00 ,  A61K39/00

Abstract: The present disclosure relates to a novel class of anti-TNFα monoclonal antibodies or antigen binding fragments comprising a homogeneous population of anti-TNFα IgG molecules having the same N-glycan on each of Fc. The antibodies of the invention can be produced from anti-TNFα monoclonal antibodies by Fc glycoengineering. The glycoantibodies of the invention may have improved therapeutic values compared to the corresponding monoclonal antibodies that have not been glycoengineered.

公开(公告)号：US11319567B2

公开(公告)日：2022-05-03

申请号：US14722612

申请日：2015-05-27

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsung-I Tsai

IPC: C12N9/14 ,  C12N9/24 ,  C12P19/14 ,  C07K16/18 ,  C07K16/28 ,  A61K39/395 ,  A61K39/42 ,  A61K45/06 ,  C07K16/00 ,  C07K16/10 ,  C07K16/24 ,  C07K16/30 ,  C07K16/32 ,  A61K39/00

Abstract: The present disclosure relates to an α-fucosidase having α-(1,2), α-(1,3), α-(1,4), and α-(1,6) fucosidase activity. The present disclosure also relates to the compositions comprising the α-fucosidase, and the methods of producing and using the α-fucosidase in cleaving α-(1,2), α-(1,3), α-(1,4), and/or α-(1,6)-linked fucoses in the glycoconjugates.

公开(公告)号：US10533034B2

公开(公告)日：2020-01-14

申请号：US15866378

申请日：2018-01-09

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey Wong ,  Alice L. Yu ,  Kun-Hsien Lin ,  Tai-Na Wu

IPC: A61K39/39 ,  C07H15/18 ,  C07H15/04 ,  A61K31/7032 ,  C07H17/02 ,  A61K39/00

Abstract: Glycosphingolipids (GSLs) compositions and methods for iNKT-independent induction of chemokines are disclosed.

公开(公告)号：US10307475B2

公开(公告)日：2019-06-04

申请号：US14182296

申请日：2014-02-18

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Che Ma ,  Cheng-Chi Wang ,  Juine-Ruey Chen

IPC: A61K39/145 ,  C12N7/00 ,  A61K39/12 ,  C07K16/10 ,  A61K39/00

Abstract: Immunogenic compositions comprising partially glycosylated viral glycoproteins for use as vaccines against viruses are provided. Vaccines formulated using mono-, di-, or tri-glycosylated viral surface glycoproteins and polypeptides provide potent and broad protection against viruses, even across strains. Pharmaceutical compositions comprising monoglycosylated hemagglutinin polypeptides and vaccines generated therefrom and methods of their use for prophylaxis or treatment of viral infections are disclosed. Methods and compositions are disclosed for influenza virus HA, NA and M2, RSV proteins F, G and SH, Dengue virus glycoproteins M or E, hepatitis C virus glycoprotein E1 or E2 and HIV glycoproteins gp120 and gp41.

公开(公告)号：US10274488B2

公开(公告)日：2019-04-30

申请号：US14182290

申请日：2014-02-18

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Susan Y. Tseng

IPC: C40B50/00 ,  C40B50/12 ,  G01N33/553 ,  C40B40/12 ,  C40B50/14 ,  G01N33/58 ,  C40B60/00 ,  H01J49/40

Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: 1) the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; 2) the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and 3) the slides are more sensitive than transparent glass slides in binding analysis.

公开(公告)号：US10155823B2

公开(公告)日：2018-12-18

申请号：US15505763

申请日：2015-08-21

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Hsin-Yu Lee

IPC: C07H3/06 ,  A61K39/00 ,  A61K47/36 ,  A61K47/64 ,  C07K14/33 ,  C07K14/34 ,  C08B37/00

Abstract: This disclosure includes an immunogenic composition containing (a) a glycan conjugate including a carrier and one or more glycans, wherein each of the one or more glycans is conjugated with the carrier through a linker, and optionally (b) an adjuvant. The one or more glycan is each a Globo H derivative.

公开(公告)号：US20180298361A1

公开(公告)日：2018-10-18

申请号：US16011622

申请日：2018-06-18

Applicant: CHO Pharma Inc. ,  Academia Sinica

Inventor： Nan-Horng Lin ,  Lin-Ya Huang ,  Sachin S. Shivatare ,  Li-Tzu Chen ,  Chi-Huey Wong ,  Chung-Yi Wu ,  Ting Cheng

IPC: C12N9/42 ,  C12P21/00 ,  C07K16/28

CPC classification number: C12N9/2434 ,  C07K16/2887 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/72 ,  C07K2317/732 ,  C07K2319/21 ,  C12P21/005 ,  C12Y302/01096

Abstract: A mutant of Endos2 includes one or more mutations in the sequence of a wild-type EndoS2 (SEQ ID NO:1), wherein the one or more mutations are in a peptide region located within residues 133-143, residues 177-182, residues 184-189, residues 221-231, and/or residues 227-237, wherein the mutant of EndoS2 has a low hydrolyzing activity and a high tranglycosylation activity, as compared to those of the wild-type EndoS2. A method for preparing an engineered glycoprotein using the mutant of EndoS2 includes coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is a glycan oxazoline.

公开(公告)号：US10000747B2

公开(公告)日：2018-06-19

申请号：US15684897

申请日：2017-08-23

Applicant: CHO Pharma Inc. ,  Academia Sinica

Inventor： Nan-Horng Lin ,  Lin-Ya Huang ,  Sachin S Shivatare ,  Li-Tzu Chen ,  Chi-Huey Wong ,  Chung-Yi Wu ,  Ting Cheng

IPC: C12N9/24 ,  C12P21/00 ,  C07K16/28 ,  C12N9/42

Abstract: A mutant of EndoS2 includes one or more mutations in the sequence of a wild-type EndoS2 (SEQ ID NO: 1), wherein the one or more mutations are in a peptide region located within residues 133-143, residues 177-182, residues 184-189, residues 221-231, and/or residues 227-237, wherein the mutant of EndoS2 has a low hydrolyzing activity and a high tranglycosylation activity, as compared to those of the wild-type EndoS2. A method for preparing an engineered glycoprotein using the mutant of EndoS2 includes coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is a glycan oxazoline.