公开(公告)号：US20200179923A1

公开(公告)日：2020-06-11

申请号：US16628474

申请日：2018-07-13

Applicant: CORNING INCORPORATED

Inventor： William Joseph Lacey ,  Gregory Roger Martin ,  Ana Maria del Pilar Pardo ,  Allison Jean Tanner

IPC: B01L3/00 ,  C12M1/24 ,  C12M1/00

Abstract: A cell culture vessel has a wall and cell culture surface having a plurality of microcavities for culturing cells in three-dimensional conformation, referred to as “spheroids”. The inner surface of the wall and the cell culture surface define a cell culture chamber of the vessel. The wall is attached to the cell culture surface in a way that does not provide flat surfaces on or around the cell culture surface so that the vessel provides an environment suitable for the production of a homogeneous population of three-dimensional cell clusters, or spheroids.

公开(公告)号：US20190322969A1

公开(公告)日：2019-10-24

申请号：US16447453

申请日：2019-06-20

Applicant: Corning Incorporated

Inventor： Ye Fang ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Gregory Roger Martin ,  Kaitlyn Mary Matias ,  Michael Kurt Schaefer ,  Allison Jean Tanner

IPC: C12M1/32 ,  C12M1/04 ,  C12M3/06 ,  C12M1/26 ,  C12M1/00

Abstract: The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.

公开(公告)号：US20180051242A1

公开(公告)日：2018-02-22

申请号：US15691102

申请日：2017-08-30

Applicant: CORNING INCORPORATED

Inventor： David Alan Kenney ,  Gregory Roger Martin ,  Allison Jean Tanner ,  Joseph Christopher Wall

IPC: C12M1/24 ,  C12M1/00 ,  C12M3/00

CPC classification number: C12M23/08 ,  C12M23/34 ,  C12M23/40 ,  C12M23/44 ,  Y10T29/49826

Abstract: A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves.

公开(公告)号：US20170135337A1

公开(公告)日：2017-05-18

申请号：US15419678

申请日：2017-01-30

Applicant: CORNING INCORPORATED

Inventor： Jessica Monique Brown ,  Nicholas Michael Illsley ,  Gregory Roger Martin ,  Ana Marie del Pilar Pardo ,  Allison Jean Tanner

IPC: A01N1/02

CPC classification number: A01N1/0284 ,  A01N1/0242 ,  A01N1/0263 ,  A01N1/0268 ,  A01N1/0273 ,  A61J1/05 ,  A61J1/1412 ,  A61J1/16 ,  A61M5/145 ,  A61M5/2425 ,  C12M23/48 ,  C12M33/04 ,  C12M45/22

Abstract: An article including, either: a frame having an interior cavity, and a flexible liner situated within the interior cavity; or a deformable frame having an interior cavity, as defined herein. Either frame can receive a sample, such as liquid having suspended live cells, and cooling freezes the sample to a solid sample. The solid sample can be readily ejected from the article having either a frame with the flexible liner, or the deformable frame, without extensive thawing. The solid sample can be rapidly displaced from the article by the methods and apparatus disclosed herein.

公开(公告)号：US20140106444A1

公开(公告)日：2014-04-17

申请号：US14132749

申请日：2013-12-18

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12N5/071

CPC classification number: C12M23/08 ,  C12M23/04 ,  C12M23/24 ,  C12M23/34 ,  C12M23/38 ,  C12M23/58 ,  C12M25/02 ,  C12M25/04 ,  C12M25/06 ,  C12M29/04 ,  C12N5/0602

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

公开(公告)号：US20130260454A1

公开(公告)日：2013-10-03

申请号：US13904171

申请日：2013-05-29

Applicant: Corning Incorporated

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/12

CPC classification number: C12M23/08 ,  C12M23/04 ,  C12M23/24 ,  C12M23/34 ,  C12M23/38 ,  C12M23/58 ,  C12M25/02 ,  C12M25/04 ,  C12M25/06 ,  C12M29/04 ,  C12N5/0602

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

公开(公告)号：US20240002778A1

公开(公告)日：2024-01-04

申请号：US18368281

申请日：2023-09-14

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/00 ,  C12M1/26 ,  C12N5/00

CPC classification number: C12M47/02 ,  C12M33/14 ,  C12N5/0075

Abstract: A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.

公开(公告)号：US20230383226A1

公开(公告)日：2023-11-30

申请号：US18031774

申请日：2021-10-27

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M3/06 ,  C12M1/32 ,  C12M1/00 ,  C12M1/34 ,  C12N5/00

CPC classification number: C12M23/16 ,  C12M23/12 ,  C12M23/20 ,  C12M41/46 ,  C12N5/0062 ,  C12M23/38 ,  C12N2513/00

Abstract: Microcavity plates (10) comprise a microcavity substrate and a base (303) comprising a bottom grid comprising a plurality of grid segments arranged in ordered rows and columns to form a plurality of openings, and an open well (307) comprising a plurality of sidewalls (309) extending vertically from a perimeter of the bottom grid. The microcavity substrate comprises a plurality of microcavities (315) arranged in ordered rows and columns that align with the plurality of openings in the bottom grid, each microcavity comprising a cavity disposed within an opening of the bottom grid. The microcavity plate may further comprise an upper grid (405) for placement on top of the bottom grid delineating the ordered rows and columns of the base.

公开(公告)号：US11788051B2

公开(公告)日：2023-10-17

申请号：US16061964

申请日：2016-12-13

Applicant: Corning Incorporated

Inventor： Gregory Roger Martin ,  Allison Jean Tanner

IPC: C12M1/00 ,  C12M1/26 ,  C12N5/00

CPC classification number: C12M47/02 ,  C12M33/14 ,  C12N5/0075

Abstract: A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.

公开(公告)号：US11767499B2

公开(公告)日：2023-09-26

申请号：US16628810

申请日：2018-07-13

Applicant: CORNING INCORPORATED

Inventor： Gregory Roger Martin ,  Ana Maria Del Pilar Pardo ,  Michael Kurt Schaefer ,  Allison Jean Tanner

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/24 ,  C12M1/04 ,  C12M1/00

CPC classification number: C12M23/16 ,  B01L3/502715 ,  C12M23/08 ,  C12M23/24 ,  C12M29/04 ,  B01L2300/0681 ,  B01L2300/12

Abstract: A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.