公开(公告)号：US20240150411A1

公开(公告)日：2024-05-09

申请号：US18337881

申请日：2023-06-20

Applicant: University of Washington ,  INSTITUTE FOR RESEARCH IN BIOMEDICINE

Inventor： Neil P. KING ,  David BAKER ,  Brooke FIALA ,  Lance Joseph STEWART ,  Laurent PEREZ ,  Antonio LANZAVECCHIA ,  Jessica MARCANDALLI

IPC: C07K14/005 ,  A61K39/12 ,  A61P31/14 ,  B82Y5/00 ,  C12N7/00

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61P31/14 ,  B82Y5/00 ,  C12N7/00 ,  A61K2039/575

Abstract: Disclosed herein are nanostructures and their use, where the nanostructures include



(a) a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides;
(b) a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides, wherein the second polypeptide differs from the first polypeptide;
wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure; and
wherein the nanostructure displays multiple copies of one or more paramyxovirus and/or pneumovirus F proteins or antigenic fragments thereof, on an exterior of the nanostructure.

公开(公告)号：US11970740B2

公开(公告)日：2024-04-30

申请号：US17392193

申请日：2021-08-02

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240123075A1

公开(公告)日：2024-04-18

申请号：US18356932

申请日：2023-07-21

Applicant: University of Washington

Inventor： Shaoyi JIANG ,  Priyesh JAIN ,  Jinrong MA ,  Bowen Li

IPC: A61K47/58 ,  A61K9/51 ,  A61L27/34 ,  A61L29/08 ,  A61L31/10 ,  C08F220/60 ,  C08J3/075 ,  C09D5/16 ,  C12N5/078

CPC classification number: A61K47/58 ,  A61K9/5138 ,  A61L27/34 ,  A61L29/085 ,  A61L31/10 ,  C08F220/60 ,  C08J3/075 ,  C09D5/16 ,  C12N5/0644 ,  C08F220/603 ,  C08F222/225 ,  C08J2300/206 ,  C08J2333/26

Abstract: N-oxide and monomers, N-oxide polymers and copolymers, methods for making the N-oxide monomers, polymers, and copolymers, compositions and materials that include N-oxide polymers and copolymers, and methods for using the N-oxide monomers, N-oxide polymers, and N-oxide copolymers.

公开(公告)号：US20240110226A1

公开(公告)日：2024-04-04

申请号：US18536721

申请日：2023-12-12

Applicant: University of Washington

Inventor： Georg Seelig ,  Richard Muscat ,  Alexander B. Rosenberg

IPC: C12Q1/6806 ,  C12N15/10 ,  C12Q1/6855

CPC classification number: C12Q1/6806 ,  C12N15/1065 ,  C12Q1/6855

Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.

公开(公告)号：US20240101615A1

公开(公告)日：2024-03-28

申请号：US18158384

申请日：2023-01-23

Applicant: UNIVERSITY OF WASHINGTON

Inventor： Zibo CHEN ,  Scott Boyken ,  Sherry Bermeo ,  Robert A. Langan ,  David Baker

IPC: C07K14/435 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  C07K14/00 ,  C07K19/00 ,  G01N33/53 ,  G06N3/00 ,  G16B5/00 ,  G16B15/00 ,  H03K19/00

CPC classification number: C07K14/435 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  C07K14/00 ,  C07K19/00 ,  G01N33/53 ,  G06N3/002 ,  G16B5/00 ,  G16B15/00 ,  H03K19/00 ,  C07K2319/00 ,  G16B5/10

Abstract: Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.

公开(公告)号：US20240087322A1

公开(公告)日：2024-03-14

申请号：US18463683

申请日：2023-09-08

Applicant: University of Washington ,  Seattle Children's Hospital d/b/a Seattle Children's Research Institute

Inventor： Daniel King ,  Blake Hannaford ,  Yangming Li ,  Lingga Adidharma ,  Randall Bly ,  Al-Waleed M. Abuzeid ,  Kristen S. Moe

IPC: G06V20/50 ,  G06N3/0464 ,  G06N3/047 ,  G06N3/088 ,  G06T7/70 ,  G06V10/764 ,  G06V10/774 ,  G06V10/82 ,  G16H70/20

CPC classification number: G06V20/50 ,  G06N3/0464 ,  G06N3/047 ,  G06N3/088 ,  G06T7/70 ,  G06V10/764 ,  G06V10/774 ,  G06V10/82 ,  G16H70/20 ,  G06T2207/10016 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30004 ,  G06V2201/034

Abstract: A method includes accessing training images that collectively depict multiple stages of a surgical procedure. The method also includes accessing labels that indicate, for each of the training images, characteristics of one or more surgical tools depicted and a stage of the multiple stages of the surgical procedure depicted. The method also includes training a computational model, using the training images and the labels, to associate runtime images with a stage of the multiple stages based on characteristics of one or more surgical tools that are depicted by the runtime images. Another method includes associating, using a computational model, runtime images with a stage of a surgical procedure based on characteristics of one or more surgical tools depicted by the runtime images and generating output that indicates the stage associated with each of the runtime images.

公开(公告)号：US11926812B2

公开(公告)日：2024-03-12

申请号：US16769569

申请日：2018-12-04

Applicant: EnTox Sciences, Inc. ,  University of Washington

Inventor： Ian Sweet ,  Gamal Khalil ,  Daniel Cook

IPC: C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12M3/06

CPC classification number: C12M29/10 ,  C12M23/16 ,  C12M23/42 ,  C12M23/44 ,  C12M29/14 ,  C12M41/32

Abstract: A microperifusion system includes at least one perifusate reservoir module having N receptacles and at least one port to communicate a pressurizing gas into or out from the respective perifusate reservoir module. A corresponding perifusion chamber module having M microperifusion channels is mechanically coupleable to at least one perifusate reservoir module to thereby form, when coupled, a sealed fluid communication between a selected first number of the N receptacles and a selected second number of the microperifusion channels. A control system module is coupleable to each perifusate reservoir and each perifusion chamber to control a flow of pressuring gas communicated into or out from the perifusate reservoir sense an effect of a microperifusion test operation occurring in each microperifusion channel.

公开(公告)号：US11919256B2

公开(公告)日：2024-03-05

申请号：US17655155

申请日：2022-03-16

Applicant: The Boeing Company ,  University of Washington

Inventor： Shuonan Dong ,  Silas L. Studley ,  Samuel F. Pedigo ,  Nathan A. Secinaro ,  Lukas Wavrin ,  Kevin Hsu ,  James Hutchinson ,  Connor Burch ,  Nini Hong ,  Devin Ide ,  Lucky Singh ,  Santosh Devasia

IPC: B29C70/30 ,  B29C70/54 ,  B29K105/08

CPC classification number: B29C70/30 ,  B29C70/545 ,  B29C2793/0081 ,  B29K2105/0881

Abstract: A manufacturing system includes a cutting machine, an adhesion machine, and a pick-and-place system. The cutting machine sequentially cuts a continuous length of a unidirectional prepreg into prepreg segments. Each prepreg segment has an opposing pair of segment cut edges that are non-parallel to a lengthwise direction of the unidirectional prepreg. The adhesion machine has a conveyor belt and an adhesion station. The pick-and-place system sequentially picks up the prepreg segments from the cutting machine, and places the prepreg segments in end-to-end relation on the conveyor belt, and in an orientation such that the segment cut edges are generally parallel to a lengthwise direction of the conveyor belt. The conveyor belt feeds the prepreg segments to the adhesion station. The adhesion station adheres the prepreg segments to a continuous length of a backing material, thereby resulting in a continuous length of a backed cross-ply prepreg.

公开(公告)号：US20240066322A1

公开(公告)日：2024-02-29

申请号：US18035612

申请日：2021-11-05

Applicant: University of Washington

Inventor： Larry A Pierce, II ,  Robert S. Miyaoka ,  Robert L Harrison

IPC: A61N5/10 ,  A61B6/00 ,  A61B6/04 ,  A61B6/03 ,  A61B5/00

CPC classification number: A61N5/1071 ,  A61B6/4266 ,  A61B6/0407 ,  A61B6/032 ,  A61B6/037 ,  A61B5/6805

Abstract: Various devices, systems, and methods for performing personalized dosimetry of a patient receiving a radiopharmaceutical are described. In an example method, anatomic data is generated by performing a computed tomography (CT) scan on the patient when they are lying down and wearing a garment. Based on the anatomic data, locations of organs of the patient are determined with respect to one or more fiducial markers integrated with the garment. Detectors for detecting photons from a radiopharmaceutical are placed on the garment based on locations of the organs. Subsequently, the patient may be administered a dose of the radiopharmaceutical. When the patient wears the garment, the detectors may detect photons released from the decaying radiopharmaceutical that is distributed in the organs. The radiation dosage to the organs may be determined based on the detected photons.

公开(公告)号：US20240066185A1

公开(公告)日：2024-02-29

申请号：US18458120

申请日：2023-08-29

Applicant: University of Washington

Inventor： Ying Zheng ,  Nicole Zeinstra ,  Ariana Frey

IPC: A61L27/38 ,  A61L27/24 ,  C12N5/071 ,  C12N5/077

CPC classification number: A61L27/3808 ,  A61L27/24 ,  A61L27/3804 ,  A61L27/3826 ,  C12N5/0658 ,  C12N5/069 ,  C12N2533/54

Abstract: Compositions and methods for modular fabrication of large, three-dimensional, perfusable networks of vascularized tissue are provided. Each layer within the tissue may include the same or different cell types and have the same or different types of vasculature. In some aspects layers of tissue together form the large, three-dimensional perfusable networks as each layer contains a portion of the larger tissue and the combination of the various layers creates a tertiary structure of integrated perfusable networks that exhibit remodeling and evenly distributed perfusion among layers while maintaining a patterned open-lumen architecture.