公开(公告)号：US10314871B2

公开(公告)日：2019-06-11

申请号：US15875098

申请日：2018-01-19

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Sandip Shinde ,  Stephen Ventre ,  Jennifer Lloyd-Randolfi ,  Michael Lamont

IPC: A61K36/02 ,  C12P7/64 ,  C11C3/00 ,  A61K8/37 ,  A61K31/231 ,  A61Q19/00

Abstract: Methods of producing high purity palmitoleic acid esters from natural oils are disclosed. The natural oils may comprise plant oil, nut oil, microalgae oil, and fish oil. The methods of processing the natural oil comprise transesterification with ethanol as the reacting solvent to produce ethyl esters. Methods of producing a high purity fraction of Omega-3, 6, & 9 fatty acid esters from natural oils are also disclosed. The high purity fatty acid esters may be used in nutrition, cosmetic, and nutraceutical products.

公开(公告)号：US10184099B2

公开(公告)日：2019-01-22

申请号：US15142811

申请日：2016-04-29

Applicant: Heliae Development, LLC

Inventor： Richard Mazur ,  Luke Cizek ,  Mason Oelschlager ,  Mason McCarty ,  Minh Duc Tram ,  Justin Hayden ,  Candyce Bair

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/02 ,  C12M1/34 ,  C12M1/04 ,  C12M1/12

Abstract: Some embodiments include a system having a support structure configured to mechanically support a first bioreactor. The support structure can have a first frame and a second frame together being configured to mechanically support the first bioreactor in interposition between the first frame and the second frame. The first frame can maintain a first set point temperature of the first bioreactor through an exchange of thermal energy between the first frame and the first bioreactor when the first bioreactor is vitally supporting one or more first microorganisms and when the support structure is mechanically supporting the first bioreactor. Other embodiments of related systems and methods are also disclosed.

公开(公告)号：US10047337B2

公开(公告)日：2018-08-14

申请号：US15142913

申请日：2016-04-29

Applicant: Heliae Development, LLC

Inventor： Luke Cizek ,  Miquel Olaizola ,  Mason McCarty ,  Mason Oelschlager

IPC: C12N1/12 ,  C12M1/00 ,  C12M3/00 ,  C12M1/04 ,  C12M1/12 ,  C12M1/02 ,  C12M1/34

CPC classification number: C12N1/12 ,  C12M21/02 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M27/04 ,  C12M29/04 ,  C12M37/00 ,  C12M41/22 ,  C12M41/36 ,  C12M41/40

Abstract: Some embodiments include a method of culturing one or more microalgae. The method can include: inoculating a bioreactor with the one or more microalgae and a fluidic support medium, the bioreactor having one or more bioreactor walls at least partially enclosing a bioreactor cavity, being configured to be at least one of folded up or rolled up, and being sterile when the bioreactor is inoculated with the one or more microalgae, the one or more bioreactor walls comprising at least one bioreactor wall material, and the at least one bioreactor wall material being flexible and at least partially transparent; and vitally supporting the one or more microalgae. Other embodiments of related systems and methods are also disclosed.

公开(公告)号：US10023830B2

公开(公告)日：2018-07-17

申请号：US14802058

申请日：2015-07-17

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Frank Mars

IPC: C12M1/00 ,  C12M1/12 ,  C12M1/18 ,  C12M1/02 ,  C12M1/34 ,  C12M1/36

Abstract: Systems and methods are disclosed for continuously optimizing the profile of an aqueous culture comprising microorganisms. The systems and methods comprise a controlled environment comprising a housing enclosing a lighting device and controlled temperature environment, and sensor modules for measuring culture and environment parameters. The sensor modules may be in communication with an automated computer control system to continuously optimize the microorganism culture profile.

公开(公告)号：US20170145378A1

公开(公告)日：2017-05-25

申请号：US15422829

申请日：2017-02-02

Applicant: Heliae Development, LLC

Inventor： Eneko GANUZA TABERNA ,  Anna Lee TONKOVICH ,  Jason D. LICAMELE

IPC: C12N1/12

Abstract: Methods of culturing microorganisms in non-axenic mixotrophic conditions are disclosed. A method of culturing microalgae mixotrophically and controlling bacterial contamination with an acetic acid/pH auxostat system is specifically described. Methods of culturing microalgae mixotrophically with an increased productivity through an increase in oxygen transfer to the culture are also described.

公开(公告)号：US20160289630A1

公开(公告)日：2016-10-06

申请号：US15143012

申请日：2016-04-29

Applicant: Heliae Development, LLC

Inventor： Luke Cizek ,  Mason Oelschlager ,  Justin Hayden ,  Mason McCarty

IPC: C12N1/12 ,  A61L2/07 ,  A61L2/08

CPC classification number: C12N1/12 ,  A61L2/07 ,  A61L2/081 ,  C12M21/02 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M37/00 ,  C12M41/22 ,  C12M41/36

Abstract: Some embodiments include a method. The method can include: conducting a first sterilization of an assembled bioreactor; after conducting the first sterilization of the assembled bioreactor, vitally supporting one or more first microorganisms with the assembled bioreactor; after vitally supporting the one or more first microorganisms with the assembled bioreactor, removing the one or more first microorganisms from the assembled bioreactor; after removing the one or more first microorganisms from the assembled bioreactor, gathering up the assembled bioreactor; after gathering up the assembled bioreactor, conducting a second sterilization of the assembled bioreactor; and after conducting the second sterilization of the assembled bioreactor, vitally supporting one or more second microorganisms with the assembled bioreactor. Other embodiments of related systems and methods are also disclosed.

Abstract translation: 一些实施例包括一种方法。 该方法可以包括：对组装的生物反应器进行第一次灭菌; 在组装的生物反应器进行第一次灭菌之后，用组装的生物反应器对一个或多个第一微生物进行极端支撑; 在用所组装的生物反应器对所述一种或多种第一微生物进行重要支持之后，从组装的生物反应器中除去所述一种或多种第一种微生物; 在从组装的生物反应器中除去一种或多种第一微生物之后，收集组装的生物反应器; 在收集组装的生物反应器之后，进行组装的生物反应器的第二次灭菌; 并且在组装的生物反应器进行第二次灭菌之后，用组装的生物反应器至少支撑一个或多个第二微生物。 还公开了相关系统和方法的其它实施例。

公开(公告)号：US20160289628A1

公开(公告)日：2016-10-06

申请号：US15142913

申请日：2016-04-29

Applicant: Heliae Development, LLC

Inventor： Luke Cizek ,  Miquel Olaizola ,  Mason McCarty ,  Mason Oelschlager

IPC: C12N1/12

CPC classification number: C12N1/12 ,  C12M21/02 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M27/04 ,  C12M29/04 ,  C12M37/00 ,  C12M41/22 ,  C12M41/36 ,  C12M41/40

Abstract: Some embodiments include a method of culturing one or more microalgae. The method can include: inoculating a bioreactor with the one or more microalgae and a fluidic support medium, the bioreactor having one or more bioreactor walls at least partially enclosing a bioreactor cavity, being configured to be at least one of folded up or rolled up, and being sterile when the bioreactor is inoculated with the one or more microalgae, the one or more bioreactor walls comprising at least one bioreactor wall material, and the at least one bioreactor wall material being flexible and at least partially transparent; and vitally supporting the one or more microalgae. Other embodiments of related systems and methods are also disclosed.

Abstract translation: 一些实施方案包括培养一种或多种微藻的方法。 该方法可以包括：用一种或多种微藻和流体支持介质接种生物反应器，所述生物反应器具有至少部分包围生物反应器腔的一个或多个生物反应器壁，其被配置为折叠或卷起至少一个， 并且当所述生物反应器接种所述一种或多种微藻时，所述一个或多个生物反应器壁包含至少一个生物反应器壁材料，并且所述至少一个生物反应器壁材料是柔性的并且至少部分透明; 并且极大地支持一种或多种微藻。 还公开了相关系统和方法的其它实施例。

公开(公告)号：US20160281051A1

公开(公告)日：2016-09-29

申请号：US14795770

申请日：2015-07-09

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Laura Carney ,  Kristine Sorensen

IPC: C12N1/12

CPC classification number: C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises detecting the contamination and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract translation: 本文描述了用过氧化氢处理冲积红球菌的培养物中污染物，特别是真菌污染物的方法。 该方法包括检测污染物，然后根据培养过程中细胞的阶段，以一定浓度的过氧化氢计量培养物，并增加细胞存活的可能性，直到累积类胡萝卜素（如虾青素）的过程 ， 做完了。

公开(公告)号：US09447375B1

公开(公告)日：2016-09-20

申请号：US14796720

申请日：2015-07-10

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Laura Carney ,  Kristine Sorensen

IPC: C12N1/12 ,  C12Q1/02

CPC classification number: C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

Abstract: Methods of treating lysis in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture comprising with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract translation: 本文描述了用过氧化氢处理流感嗜血杆菌的培养物中的裂解方法。 该方法包括在培养过程中基于细胞阶段的一定浓度的过氧化氢给予培养物，并且频率增加细胞存活的可能性，直到累积类胡萝卜素（如虾青素）的过程完成。

公开(公告)号：US09447373B1

公开(公告)日：2016-09-20

申请号：US14795675

申请日：2015-07-09

Applicant: HELIAE DEVELOPMENT LLC

Inventor： Laura Carney ,  Kristine Sorensen

IPC: C12N1/12 ,  C12Q1/06

CPC classification number: C12N1/12 ,  A01N59/08 ,  C12N1/38 ,  C12P23/00 ,  C12P33/00 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/06 ,  G01N2333/405

Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Abstract translation: 本文描述了用过氧化氢和盐处理红潮球红藻的培养物中的污染物，特别是真菌污染物的方法。 该方法包括在培养过程中基于细胞阶段，并以增加细胞存活的可能性的频率，将包含一定浓度的过氧化氢浓度的培养物给药，直到累积类胡萝卜素如虾青素的过程 ， 做完了。