公开(公告)号：US09377455B2

公开(公告)日：2016-06-28

申请号：US14746276

申请日：2015-06-22

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.

公开(公告)号：US20160074863A1

公开(公告)日：2016-03-17

申请号：US14942125

申请日：2015-11-16

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Ryan A. Sturmer ,  Vijay Srinivasan ,  Arjun Sudarsan

IPC: B01L3/00 ,  G01N27/04

CPC classification number: B01L3/50273 ,  B01L3/502792 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0427 ,  G01N27/048 ,  G01R27/16

Abstract: Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.

Abstract translation: 方法包括测量由激发信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。 还公开了存储用于执行该方法的处理器可执行指令的计算机可读介质，以及系统。 系统包括存储在存储器中的处理器，存储器和代码，当执行时，处理器至少要使接收输出电压信号，将激励信号叠加在输出电压信号上，产生叠加信号，将叠加的信号连接到 液滴致动器中的电极，当检测到电极的阻抗时抑制输出电压信号，并测量由激励信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。

公开(公告)号：US20150174578A1

公开(公告)日：2015-06-25

申请号：US14590470

申请日：2015-01-06

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Zishan Hua ,  Arjun Sudarsan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

Abstract translation: 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。

公开(公告)号：US20150021189A1

公开(公告)日：2015-01-22

申请号：US14455645

申请日：2014-08-08

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Ramakrishna Sista ,  Michael G. Pollack

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/502761 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0448

Abstract: A droplet actuator configured to improve the throughput of droplet operations in a detection spot of the droplet actuator and/or to reduce carryover problems is provided. The droplet actuator may include electrodes configured for effecting droplet operations transporting droplets on a surface; a sensor arranged in proximity to one or more of the electrodes establishing a detection window on the surface for detection of one or more properties of one or more droplets on the surface; wherein the electrodes may establish at least two pathways for transport of droplets into the detection window.

Abstract translation: 提供了一种配置成提高液滴致动器的检测点中的液滴操作的吞吐量和/或减少携带问题的液滴致动器。 液滴致动器可以包括被配置为用于实现在表面上传送液滴的液滴操作的电极; 设置在一个或多个电极附近的传感器，在表面上建立检测窗口，以检测表面上的一个或多个液滴的一个或多个特性; 其中电极可以建立用于将液滴输送到检测窗中的至少两个路径。

公开(公告)号：US20140332380A1

公开(公告)日：2014-11-13

申请号：US14338889

申请日：2014-07-23

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2300/0627 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0809 ,  B01L2400/0427 ,  G01N27/22 ,  G01N27/223 ,  G01N27/227 ,  G01N2203/00 ,  G01V3/02

Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

Abstract translation: 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。