Process for racemizing an optically active N-benzylidene amino-acid amide
    70.
    发明授权
    Process for racemizing an optically active N-benzylidene amino-acid amide 失效
    使光学活性的N-亚苄基氨基酸酰胺外消旋化的方法

    公开(公告)号:US4847412A

    公开(公告)日:1989-07-11

    申请号:US850157

    申请日:1986-04-10

    CPC classification number: C12P13/04 C12P13/08 C12P41/007

    Abstract: The invention relates to a process for racemizing an optically active N-benzylidene amino-acid amide, characterized in that a solution of the N-benzylidene amino-acid amide is mixed in a water-miscible organic solvent with at least 0.05 mole strong base per liter solution.The invention further relates to a process for preparing an L-amino acid by enzymatic separation of the corresponding DL-amino-acid amide with an enzyme preparation from Pseudomonas putida, in which process also unconverted D-amino-acid amide is left behind in solution, characterized in that benzaldehyde is added to the solution, during which addition a precipitate of D-N-benzylidene amino-acid amide is being formed, this precipitate is subsequently, after being separated off, dissolved in an acetone-water mixture, 0.08-0.15 mole KOH/liter solution is subsequently added, the resulting solution is stirred for 1-20 hours at 20.degree.-60.degree. C., sulphuric acid is then added until the pH of the solution is 5 and the resulting sulphuric acid salt of the DL-amino-acid amide is finally, after isolation at pH 8-10, converted into the DL-amino-acid amide and this DL-amino-acid amide is used again.

    Abstract translation: 本发明涉及一种使光学活性的N-亚苄基氨基酸酰胺外消旋化的方法,其特征在于将N-亚苄基氨基酸酰胺的溶液与水混溶的有机溶剂混合至少0.05摩尔强碱 升解决方案。 本发明还涉及通过用恶臭假单胞菌(Pseudomonas putida)的酶制剂酶分离相应的DL-氨基酸酰胺来制备L-氨基酸的方法,其中将未转化的D-氨基酸酰胺留在溶液中 其特征在于向溶液中加入苯甲醛,在此期间加入了DN-亚苄基氨基酸酰胺的沉淀物,然后将该沉淀物分离后溶解在丙酮 - 水混合物中,0.08-0.15摩尔 随后加入KOH /升溶液,所得溶液在20-60℃下搅拌1-20小时,然后加入硫酸直到溶液的pH为5,得到的DL- 氨基酸酰胺最后在pH8-10分离后,转化成DL-氨基酸酰胺,再次使用该DL-氨基酸酰胺。

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