公开(公告)号：US20190277843A1

公开(公告)日：2019-09-12

申请号：US16365579

申请日：2019-03-26

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Chung-YI WU ,  Susan Y. TSENG

IPC: G01N33/553 ,  G01N33/58 ,  C40B50/14 ,  C40B40/12

Abstract: Aluminum coated glass slides provide a novel glycan array platform. Specifically, aluminum coated glass slides increase sensitivity of fluorescent based assay methods. Additionally, aluminum coated glass slides allows for mass spectroscopic analysis of carbohydrates and provide a platform for examining activity of cellulases. The unique properties of ACG slides include: the metal oxide layer on the surface can be activated for grafting organic compounds such as modified oligosaccharides; the surface remains electrically conductive, and the grafted oligosaccharides can be simultaneously characterized by mass spectrometry and carbohydrate-binding assay; and the slides are more sensitive than transparent glass slides in binding analysis.

公开(公告)号：US20190275345A1

公开(公告)日：2019-09-12

申请号：US16347327

申请日：2017-11-03

Applicant: Academia Sinica ,  National Taiwan University

Inventor： Pan-Chyr Yang ,  S.-Ja Tseng ,  Zi-Xian Liao

IPC: A61N5/06 ,  A61K47/69 ,  A61K41/00 ,  A61P35/00

Abstract: Ironized viral particles such as ironized adeno-associated viral particles, which may carry a photosensitizer such as a KillerRed protein, and uses thereof in light-triggered virotherapy against tumor.

公开(公告)号：US10407673B2

公开(公告)日：2019-09-10

申请号：US16011622

申请日：2018-06-18

Applicant: CHO Pharma Inc. ,  Academia Sinica

Inventor： Nan-Horng Lin ,  Lin-Ya Huang ,  Sachin S Shivatare ,  Li-Tzu Chen ,  Chi-Huey Wong ,  Chung-Yi Wu ,  Ting Cheng

IPC: C12N9/42 ,  C12P21/00 ,  C07K16/28

Abstract: A mutant of EndoS2 includes one or more mutations in the sequence of a wild-type EndoS2 (SEQ ID NO:1), wherein the one or more mutations are in a peptide region located within residues 133-143, residues 177-182, residues 184-189, residues 221-231, and/or residues 227-237, wherein the mutant of EndoS2 has a low hydrolyzing activity and a high tranglycosylation activity, as compared to those of the wild-type EndoS2. A method for preparing an engineered glycoprotein using the mutant of EndoS2 includes coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is a glycan oxazoline.

公开(公告)号：US20190265212A1

公开(公告)日：2019-08-29

申请号：US16412704

申请日：2019-05-15

Applicant: ACADEMIA SINICA

Inventor： Chung-Hsuan CHEN ,  Chen-Yu HSIEH ,  Jung-Lee LIN

IPC: G01N30/74

Abstract: Apparatus and methods for high performance liquid chromatography. The apparatus includes a preparation loop comprising two linear stepping pumps, a sample loop comprising a sample injector, a chromatography column, and a detector device. The detector device can include a flash lamp, a flow cell, and a light sensor comprising an entrance slit, a grating; and a charge-coupled device array.

公开(公告)号：US10391163B2

公开(公告)日：2019-08-27

申请号：US15756774

申请日：2016-09-09

Applicant: Academia Sinica

Inventor： Shu-Mei Liang ,  Pei-Wen Hsiao ,  Ming-Chu Cheng ,  Yu-Chih Yang

IPC: A61K39/145 ,  C12N7/00 ,  A61K39/12 ,  A61K39/135 ,  A61K39/00 ,  A61P31/16

Abstract: A vaccine composition is disclosed. The vaccine composition comprises: (a) a therapeutically effective amount of an influenza virus-like particle (VLP) comprising: (i) influenza M1, influenza M2, influenza hemagglutinin (HA), and influenza neuraminidase (NA) proteins; (b) Foot-and-mouth disease virus (FMDV) capsid protein VP3, recombinant FMDV VP3 (rVP3), VP3 peptide, or SUMO VP3; and (c) alum. Also disclosed is use of a vaccine composition according to the invention in the manufacture of a medicament for inducing an immunogenic response in a subject in need thereof.

公开(公告)号：US10379045B2

公开(公告)日：2019-08-13

申请号：US14476191

申请日：2014-09-03

Applicant: Academia Sinica

Inventor： Pei-Kuen Wei ,  Kuang-Li Lee

IPC: G01N21/59

Abstract: The present invention provides a label-free sensing chip for identifying a chemical substance, comprising: (a) a transparent substrate comprising a base and first periodic ridges; and (b) a metal layer covering said transparent substrate, comprising second periodic ridges and third periodic ridges, in which said second periodic ridges has a height equal to or greater than the height of the first periodic ridges, and each ridge of the second periodic ridges fits into the space between each ridge of the first periodic ridges, and said third periodic ridges correspondingly located on said first periodic ridges. The present invention also provides a method for identifying a chemical substance by using the foresaid label-free sensing chip.

公开(公告)号：US10336815B2

公开(公告)日：2019-07-02

申请号：US15232819

申请日：2016-08-10

Applicant: Academia Sinica

Inventor： An-Suei Yang ,  Ing-Chien Chen ,  Chao-Ping Tung ,  Chung-Ming Yu

IPC: C07K16/00 ,  C07K16/10 ,  C12N15/10

Abstract: Disclosed herein are phage-displayed single-chain variable fragment (scFv) libraries, which comprised a plurality of scFvs with a specific sequence in each CDR. The present scFv libraries could be used to efficiently produce different antibodies with high binding affinity to H1 hemagglutinin of influenza virus. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

公开(公告)号：US10336784B2

公开(公告)日：2019-07-02

申请号：US15453836

申请日：2017-03-08

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Chung-Yi Wu ,  Sachin S. Shivatare

IPC: C07H5/06 ,  A61K31/702 ,  C12N9/10 ,  C07K16/10 ,  C12P19/04 ,  G01N33/50 ,  G01N33/532 ,  G01N33/68 ,  C08B37/00 ,  G01N33/569 ,  C12P19/28

Abstract: The present disclosure relates to novel modular methods for generating a diversity of N-glycans of high mannose, hybrid and complex types. The present disclosure also relates to exemplary arrays of the synthesized N-glycans spotted onto aluminium oxide coated slides. These arrays can be used to detect and analyze binding interactions between the synthesized N-glycans and glycan binding molecules, such as HIV-1 neutralizing antibodies. The present disclosure also relates to methods for identifying agents that bind to various types of molecules on the arrays and to defining the structural elements of the molecules on the arrays that bind to those agents. The arrays and methods provided herein may be used for general epitope identification, drug discovery and as analytical tools. The present disclosure also provides useful glycans and epitope determinants that are useful in detecting, diagnosing, recurrence monitoring and preventing pathological diseases such as HIV.

公开(公告)号：US20190177435A1

公开(公告)日：2019-06-13

申请号：US16184203

申请日：2018-11-08

Applicant: Academia Sinica

Inventor： Chi-Huey Wong ,  Tsui-Ling Hsu ,  Yi-Wei Lou ,  Chih-Wei Lin ,  Shih-Chi Yeh ,  Chung-Yi Wu ,  Han-Chung Wu

IPC: C07K16/44 ,  C07K16/30 ,  G01N33/574 ,  C07K16/18

Abstract: Pharmaceutical composition comprising antibodies or antigen binding fragments thereof that bind to globo H, SSEA3, and SSEA-4 are disclosed herein, as well as methods of use thereof. Methods of use include, without limitation, cancer therapies and diagnostics. The antibodies of the disclosure can bind to certain cancer cell surfaces. Exemplary targets of the antibodies disclosed herein can include carcinomas, such as those in brain, skin, bone, lungs, breast, esophagus, stomach, liver, bile duct, pancreas, colon, kidney, cervical, ovarian, and/or prostate cancer.

公开(公告)号：US10317379B2

公开(公告)日：2019-06-11

申请号：US14551050

申请日：2014-11-23

Applicant: Academia Sinica

Inventor： Chung-Hsuan Chen ,  Chen-Yu Hsieh ,  Jung-Lee Lin

IPC: G01N21/33 ,  G01N30/02 ,  G01N30/74

Abstract: Apparatus and methods for high performance liquid chromatography. The apparatus includes a preparation loop comprising two linear stepping pumps, a sample loop comprising a sample injector, a chromatography column, and a detector device. The detector device can include a flash lamp, a flow cell, and a light sensor comprising an entrance slit, a grating; and a charge-coupled device array.