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公开(公告)号：US07927846B2
公开(公告)日：2011-04-19
申请号：US12429499
申请日：2009-04-24
Applicant: Robert DiCosimo , Anna Panova , Samuel D. Arthur , Henry Keith Chenault
Inventor： Robert DiCosimo , Anna Panova , Samuel D. Arthur , Henry Keith Chenault
CPC classification number: C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US07910347B1
公开(公告)日：2011-03-22
申请号：US12632446
申请日：2009-12-07
Applicant: Robert DiCosimo , John Edward Gavagan , Mark S. Payne
Inventor： Robert DiCosimo , John Edward Gavagan , Mark S. Payne
CPC classification number: A61L2/22 , A61L2/186 , A61L2202/11 , A61L2202/17 , A61L2202/26 , C11D3/38654 , C12N9/18 , C12P7/40
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
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公开(公告)号：US07875443B2
公开(公告)日：2011-01-25
申请号：US12429478
申请日：2009-04-24
Applicant: Robert DiCosimo , Anna Panova , Samuel D. Arthur , Henry Keith Chenault
Inventor： Robert DiCosimo , Anna Panova , Samuel D. Arthur , Henry Keith Chenault
CPC classification number: C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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公开(公告)号：US07867748B2
公开(公告)日：2011-01-11
申请号：US12698775
申请日：2010-02-02
Applicant: Robert DiCosimo , Arie Ben-Bassat
Inventor： Robert DiCosimo , Arie Ben-Bassat
CPC classification number: C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
Abstract: The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein.
Abstract translation: 本发明涉及一种提高具有腈水解酶活性的脱水酶催化剂的比活性的方法，所述脱水酶催化剂在补液时将乙醇腈水解成乙醇酸。 特别地，提供了一种方法，其包括用戊二醛预处理具有腈水解酶活性的酶催化剂，在脱水之前固定化古巴醛预处理的酶催化剂和化学交联酶催化剂。 在再水合时，与不进行本文所述处理的具有脱水和再水合的腈水解酶活性的酶催化剂相比，酶催化剂表现出提高的特异性腈水解酶活性。
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公开(公告)号：US20100196976A1
公开(公告)日：2010-08-05
申请号：US12429499
申请日：2009-04-24
Applicant: ROBERT DICOSIMO , Anna Panova , Henry Keith Chenault , Samuel David Arthur
Inventor： ROBERT DICOSIMO , Anna Panova , Henry Keith Chenault , Samuel David Arthur
IPC: C12P7/42
CPC classification number: C12N9/78 , C12N9/96 , C12P7/42 , C12Y305/05001
Abstract: A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst.
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Patent Agency Ranking
公开(公告)号：US07741088B2
公开(公告)日：2010-06-22
申请号：US11930744
申请日：2007-10-31
Applicant: Robert DiCosimo , Arie Ben-Bassat
Inventor： Robert DiCosimo , Arie Ben-Bassat
CPC classification number: C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
Abstract: The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein.
Abstract translation: 本发明涉及一种提高具有腈水解酶活性的脱水酶催化剂的比活性的方法，所述脱水酶催化剂在补液时将乙醇腈水解成乙醇酸。 特别地，提供了一种方法，其包括用戊二醛预处理具有腈水解酶活性的酶催化剂，在脱水之前固定化古巴醛预处理的酶催化剂和化学交联酶催化剂。 在再水合时，与不进行本文所述处理的具有脱水和再水合的腈水解酶活性的酶催化剂相比，酶催化剂表现出提高的特异性腈水解酶活性。
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77.发明授权公开(公告)号：US07732172B2
公开(公告)日：2010-06-08
申请号：US12189906
申请日：2008-08-12
Applicant: Robert DiCosimo , Anna Panova , Jeffrey Scott Thompson , Robert D. Fallon , F. Glenn Gallagher , Thomas Foo , Xu Li , George C. Fox , Joseph J. Zaher , Mark S. Payne , Daniel P. O'Keefe
Inventor： Robert DiCosimo , Anna Panova , Jeffrey Scott Thompson , Robert D. Fallon , F. Glenn Gallagher , Thomas Foo , Xu Li , George C. Fox , Joseph J. Zaher , Mark S. Payne , Daniel P. O'Keefe
CPC classification number: C07C51/48 , C07C51/42 , C07C51/487 , C12P7/42 , C07C59/06
Abstract: A process is provided for producing glycolic acid from formaldehyde and hydrogen cyanide. More specifically, heat-treated formaldehyde and hydrogen cyanide are reacted to produce glycolonitrile having low concentrations of impurities. The glycolonitrile is subsequently converted to an aqueous solution of ammonium glycolate using an enzyme catalyst having nitrilase activity derived from Acidovorax facilis 72W (ATCC 57746). Glycolic acid is recovered in the form of the acid or salt from the aqueous ammonium glycolate solution using a variety of methods described herein.
Abstract translation: 提供从甲醛和氰化氢生产乙醇酸的方法。 更具体地，使热处理的甲醛和氰化氢反应以制备具有低浓度杂质的乙醇腈。 随后使用具有源自Acidovorax facilis 72W（ATCC 57746）的腈水解酶活性的酶催化剂将乙醇腈转化为乙醇酸铵水溶液。 使用本文所述的各种方法，从乙醇酸水溶液中以酸或盐的形式回收乙醇酸。
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公开(公告)号：US07723083B2
公开(公告)日：2010-05-25
申请号：US12143375
申请日：2008-06-20
Applicant: Robert DiCosimo , John E. Gavagan , Mark S. Payne , Frederick B. Cooling, III
Inventor： Robert DiCosimo , John E. Gavagan , Mark S. Payne , Frederick B. Cooling, III
CPC classification number: A01N37/02 , A01N37/16 , A01N37/36 , A01N59/00 , A61L2/186 , C12P7/40 , C12P7/62 , A01N63/02 , A01N2300/00
Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US20100086535A1
公开(公告)日：2010-04-08
申请号：US12572086
申请日：2009-10-01
Applicant: Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
Inventor： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
CPC classification number: C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
Abstract: Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
Abstract translation: 本文公开了一种在具有羧酸酯的制剂中稳定CE-7酯酶的过水解酶活性的方法，其使用向CE-7酯酶和羧酸的混合物中添加基本上未溶解的缓冲剂 酯。 此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。
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公开(公告)号：US20100086534A1
公开(公告)日：2010-04-08
申请号：US12572070
申请日：2009-10-01
Applicant: Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
Inventor： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
CPC classification number: C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
Abstract: Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
Abstract translation: 本文公开了包含至少一种CE-7酯酶，至少一种寡糖赋形剂和任选的至少一种表面活性剂的喷雾干燥制剂的酶粉末。 本文还公开了使用上述酶粉末从羧酸酯制备过氧羧酸的方法。 此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。
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