公开(公告)号：US4628026A

公开(公告)日：1986-12-09

申请号：US552090

申请日：1983-11-15

Applicant: Dietlind Gardell ,  Gail Rock

Inventor： Dietlind Gardell ,  Gail Rock

IPC: G01N21/25 ,  G01N21/64 ,  G01N33/53 ,  G01N33/554

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2201/0446 ,  G01N2201/0484 ,  Y10S435/963 ,  Y10S435/966 ,  Y10S435/973 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/821

Abstract: An automated system for rapid sequential photometric analysis of a collection of double fluorochrome stained lymphocyte specimens, useful for antibody screening or lymphocytotoxicity analysis. The specimens are sequentially alternately irradiated with light of two distinguishable wavelengths, producing fluorescence at two distinguishable wavelengths. The fluorescent emission light intensity for each irradiation of each specimen is measured using a photometer and computer. The computer controls the synchronization of the irradiation through alternately selected condenser sets with the sequential movement of specimens into the optical path of the irradiating and detected light, and calculates the quotient of the light intensities emitted from each specimen at the two selected fluorescent light wavelengths. These quotients are compared against a control ratio (for lymphocytotoxicity analysis) to classify the specimen. Also described is a method of preparing specimens for such analysis, which requires that a complement be added to the first staining solution after the latter is applied to the specimens, then this combination agitated, and then the second staining solution added and the specimen incubated.

Abstract translation: 一种用于快速顺序光度分析双重荧光染色淋巴细胞标本的自动化系统，可用于抗体筛选或淋巴细胞毒性分析。 用两个可辨别的波长的光顺序地交替地照射样品，在两个可区别的波长处产生荧光。 使用光度计和计算机测量每个样品的每次照射的荧光发射光强度。 计算机通过交替选择的聚光器组来控制照射的同步，同时将样品顺序移动到照射和检测光的光路中，并计算在两个选定的荧光波长处从每个样品发射的光强度的商。 将这些商与对照比（用于淋巴细胞毒性分析）进行比较以对样品进行分类。 还描述了制备用于这种分析的样品的方法，其要求在将样品应用于样品后将补体添加到第一染色溶液中，然后将该组合搅拌，然后加入第二染色溶液并将样品孵育。

公开(公告)号：US12038429B2

公开(公告)日：2024-07-16

申请号：US15907695

申请日：2018-02-28

Applicant: Specific Technologies, LLC

Inventor： Paul A. Rhodes ,  Soumitesh Chakravorty ,  Sung Hyun Lim

IPC: G01N33/50 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q3/00 ,  G01N21/03 ,  G01N21/78 ,  G01N21/01 ,  G01N21/80

CPC classification number: G01N33/5008 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q3/00 ,  G01N21/03 ,  G01N21/78 ,  G01N2021/0118 ,  G01N21/80 ,  G01N2201/0446

Abstract: Devices, systems, and methods for strain-specific identification and assessment of susceptibility of microorganisms based on the response of sensors in a colorimetric sensor array to metabolic products of the microorganism. An exemplary method includes culturing a sample containing microorganisms in a medium and in gaseous communication with a colorimetric sensor array. Sensors in the colorimetric sensor array are exposed to volatile organic compounds produced by the microorganism. The method then includes assessing a resistance of the microorganism to at least one substance. The resistance is assessed based on a response of the sensors in the colorimetric sensor array to the volatile organic compounds produced by the microorganism.

公开(公告)号：US11953439B2

公开(公告)日：2024-04-09

申请号：US16543254

申请日：2019-08-16

Applicant: Massachusetts Institute of Technology ,  The Penn State Research Foundation

Inventor： Lauren Dell Zarzar ,  Sara N. Nagelberg ,  Mathias Kolle ,  Amy Goodling

IPC: G01N21/64 ,  B29D11/00

CPC classification number: G01N21/6452 ,  B29D11/00298 ,  G01N2201/0446

Abstract: The present invention generally relates to the generation of tunable coloration and/or interference from, for example, surfaces, emulsion droplets and particles. Embodiments described herein may be useful for generation of tunable electromagnetic radiation such as coloration (e.g., iridescence, structural color) and/or interference patterns from, for example, surfaces (e.g., comprising a plurality of microdomes and/or microwells), emulsion droplets and/or particles. In some embodiments, the surfaces, interfaces, droplets, and/or particles produce visible color (e.g., structural color) without the need for dyes.

公开(公告)号：US20240085332A1

公开(公告)日：2024-03-14

申请号：US18467469

申请日：2023-09-14

Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA ,  THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： William Benman ,  Lukasz Bugaj ,  Brian Chow ,  Saachi Datta ,  David Gonzalez-Martinez ,  Jose Avalos

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N2201/0446 ,  G01N2201/0627 ,  G01N2201/064

Abstract: Provided herein is a stimulation and measurement device. The stimulation and measurement device includes a microwell plate having a top side, a bottom side, and at least one well extending from the top side towards the bottom side; a first assembly removably positioned on the top side of the microwell plate; a second assembly removably positioned on the bottom side of the microwell plate; and a microcontroller. The first assembly includes a first printed circuit board having at least one pair of stimulating light emitting diodes (LEDs) positioned thereon to align with one of the wells. The second assembly includes a second printed circuit board having at least one photodiode positioned thereon to align with one of the pairs of stimulating LEDs, and at least one excitation LED paired with each of the photodiodes. The microcontroller is configured to control the stimulating LED(s), the photodiode(s), and the excitation LED(s).

公开(公告)号：US20180100798A1

公开(公告)日：2018-04-12

申请号：US15565161

申请日：2016-04-08

Applicant: BACTUSENSE TECHNOLOGIES LTD.

Inventor： OFER DU-NOUR

IPC: G01N21/47 ,  A61B5/00 ,  G02B21/06 ,  G01D5/26 ,  G02B21/36

CPC classification number: G01N21/4788 ,  A61B5/7257 ,  G01D5/266 ,  G01N21/45 ,  G01N21/4738 ,  G01N2021/458 ,  G01N2021/4726 ,  G01N2021/4797 ,  G01N2201/0446 ,  G02B21/06 ,  G02B21/362

Abstract: Systems for the monitoring of bacterial levels in samples, using spectral analysis of the light diffracted from a substrate with an ordered array of pores having diameters enabling the targets to enter them. The trapping pore array is cyclically illuminated by light of different wavelengths, and the light diffracted from the pore array is imaged by a 2-dimensional detector array, with one pixel, or a small group of pixels receiving light from each associated pore. The temporal sequence of frames provides a series of images, each from the reflection of a different wavelength. A time sequenced readout of the signal from the pixel or pixels associated with each pore region, provides a spectral plot of the reflected light from that pore region. Spectral analysis of the light intensity from this series of different wavelength enables the effective optical thickness (EOT) of each pore to be extracted.

公开(公告)号：US09909979B2

公开(公告)日：2018-03-06

申请号：US15046354

申请日：2016-02-17

Applicant: Engineered Medical Technologies

Inventor： Bryan John Cowger ,  Megan Anzar Babb ,  Peichen Chang

IPC: G01N21/00 ,  G01N21/33 ,  G01J1/42 ,  G01N21/03 ,  G01N21/13 ,  G01N21/31 ,  G01N21/17

CPC classification number: G01N21/33 ,  G01J1/42 ,  G01N21/03 ,  G01N21/13 ,  G01N21/31 ,  G01N2021/0367 ,  G01N2021/1751 ,  G01N2201/0446 ,  G01N2201/062

Abstract: The present describes a system and method for determining the concentration of tetrahydrocannabinol (THC) including a tray comprising a first analyte including an infusion of a solvent and cannabis, a light emitting element configured to illuminate the first analyte, a light receiving element configured to receive a first light transmitted through the first analyte, and a control circuit configured to calculate a concentration of tetrahydrocannabinol in the first analyte based at least in part on the first light.

公开(公告)号：US09435734B2

公开(公告)日：2016-09-06

申请号：US14806999

申请日：2015-07-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Norikazu Sugiyama ,  Takuji Kataoka ,  Ikuo Arata

IPC: G01N21/01 ,  G01N21/47 ,  G01N33/483 ,  G02B21/08 ,  G01N21/49 ,  G01N33/50 ,  G01N21/59 ,  G02B21/36

CPC classification number: G01N21/47 ,  G01N21/49 ,  G01N21/59 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2201/0446 ,  G01N2201/062 ,  G02B21/088 ,  G02B21/36

Abstract: A method for observing stem cells by an observation device 1 comprises, placing stem cells C in a petri dish 11, mounting the petri dish 11 on a waveguide 21 via water 13, emitting illumination light L1 into the waveguide 21 and emitting the illumination light L1 to the stem cells C in the petri dish 11 via the water 13, and detecting scattered light L2, the scattered light L2 being the illumination light L1 emitted to the stem cells C that is scattered by the stem cells C and has passed through the waveguide 21. Then, in the light image detected by means of the scattered light L2, a region that is markedly darker than other regions is identified as being in the state tending toward differentiation.

公开(公告)号：US20160238623A1

公开(公告)日：2016-08-18

申请号：US15136610

申请日：2016-04-22

Applicant: Fluidigm Corporation

Inventor： Gang Sun ,  Greg Harris ,  Andy May ,  Kyle Self ,  Kevin Farrell ,  Paul Wyatt

IPC: G01N35/00 ,  G01N21/64 ,  C12Q1/68 ,  B01L3/00 ,  G01N35/10

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: A system includes a platform including one or more workstations and a microfluidic input device coupled to the one or more workstations. The microfluidic input device is adapted to receive a microfluidic device from a user. The system also includes a robotic device comprising a robotic arm and disposed on the platform. The robotic arm is capable of accessing the one or more workstations and is configured to transfer a plurality of sample solutions from a first spatial location to the microfluidic device when coupled to the microfluidic input device. The system further includes a multi-pixel image capturing device optically coupled to the microfluidic device and an image processing device operably coupled to the multi-pixel image capturing device. The multi-pixel image capturing device is adapted to capture a plurality of multi-pixel images. The image processing device is configured to receive the plurality of multi-pixel images.

Abstract translation: 系统包括包括一个或多个工作站的平台和耦合到该一个或多个工作站的微流体输入设备。 微流体输入装置适于从用户接收微流体装置。 该系统还包括机器人装置，其包括机器臂并且设置在平台上。 机器臂能够访问一个或多个工作站，并且被配置为当耦合到微流体输入装置时将多个样品溶液从第一空间位置传送到微流体装置。 该系统还包括光学耦合到微流体装置的多像素图像捕获装置和可操作地耦合到多像素图像捕获装置的图像处理装置。 多像素图像捕获装置适于捕获多个多像素图像。 图像处理装置被配置为接收多个多像素图像。

公开(公告)号：US20160216503A1

公开(公告)日：2016-07-28

申请号：US15007196

申请日：2016-01-26

Applicant: California Institute of Technology

Inventor： Jinho Kim ,  Changhuei Yang

IPC: G02B21/36 ,  G02B21/00 ,  G02B21/16

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2021/6478 ,  G01N2201/0446 ,  G02B13/0095 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/367 ,  H04N5/22541 ,  H04N5/2256 ,  H04N5/2258

Abstract: In one aspect an imaging system includes: an illumination system including an array of light sources; an optical system including one or more lens arrays, each of the lens arrays including an array of lenses, each of the lenses in each of the one or more lens arrays in alignment with a corresponding set of light sources of the array of light sources; an imaging system including an array of image sensors, each of the image sensors in alignment with a corresponding lens or set of lenses of the one or more lens arrays, each of the image sensors configured to acquire image data based on the light received from the corresponding lens or set of lenses; a plate receiver system capable of receiving a multi-well plate including an array of wells, the plate receiver system configured to align each of the wells with a corresponding one of the image sensors; and a controller configured to control the illumination of the light sources and the acquisition of image data by the image sensors, the controller further configured to perform: an image acquisition process including a plurality of scans, each scan associated with a unique pattern of illumination, each of the image sensors configured to generate an image for a respective one of the wells during each scan; and an image reconstruction process during which the controller performs a fourier ptychographic operation to generate a reconstructed image for each of the wells based on the image data captured for the respective well during each of the scans.

Abstract translation: 在一个方面，成像系统包括：包括光源阵列的照明系统; 包括一个或多个透镜阵列的光学系统，每个透镜阵列包括透镜阵列，所述一个或多个透镜阵列中的每一个中的每个透镜与所述光源阵列的相应组的光源对准; 一种成像系统，包括图像传感器阵列，每个图像传感器与一个或多个透镜阵列的对应透镜或透镜组对准，每个图像传感器被配置为基于从所述一个或多个透镜阵列接收的光获取图像数据 相应的镜头或镜片组; 板接收器系统，其能够接收包括孔阵列的多孔板，所述板接收器系统被配置为使每个所述孔与所述图像传感器中的相应一个对准; 以及控制器，其被配置为控制所述光源的照明和由所述图像传感器获取图像数据，所述控制器还被配置为执行：包括多个扫描的图像获取处理，每个扫描与唯一的照明模式相关联， 每个图像传感器被配置为在每次扫描期间为相应的一个孔产生图像; 以及图像重建过程，在该图像重建过程期间，控制器基于在每个扫描期间针对相应的阱捕获的图像数据执行傅立叶ptychographic操作以生成每个孔的重建图像。

公开(公告)号：US20160209328A1

公开(公告)日：2016-07-21

申请号：US14913148

申请日：2014-08-19

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： Klaus W. Berndt ,  Timothy R. Hansen

IPC: G01N21/51 ,  G01N15/06

CPC classification number: G01N21/51 ,  G01N15/06 ,  G01N2015/0693 ,  G01N2021/4709 ,  G01N2021/4761 ,  G01N2021/513 ,  G01N2201/0446 ,  G01N2201/062

Abstract: Described are devices, methods, and systems that are suitable for rapidly and simultaneously determining the concentration of suspended particles in a sample. The devices, methods, and systems allow for the rapid and simultaneous interrogation of a large number of sample wells in a single vessel, for example, samples contained in a two-dimensional array or micro-titer plate, without the need for moving reading heads or moving the sample vessel. The nephelometry system allows the user to rapidly and simultaneously measure the concentration of the particles in numerous samples, adjust the concentration of the particles in the sample with a sample handling system, and re-measure the concentration of the samples in order to achieve a desired concentration.

Abstract translation: 描述了适于快速并同时确定样品中悬浮颗粒浓度的装置，方法和系统。 装置，方法和系统允许在单个容器中快速和同时询问大量样品孔，例如包含在二维阵列或微滴定板中的样品，而不需要移动读取头 或移动样品容器。 比浊法系统允许用户快速并同时测量多个样品中的颗粒浓度，用样品处理系统调节样品中颗粒的浓度，并重新测量样品的浓度以达到所需的 浓度。