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    NUCLEIC ACID PURIFICATION METHOD
    71.
    发明申请
    NUCLEIC ACID PURIFICATION METHOD 有权
    核酸纯化方法

    公开(公告)号：US20120245337A1

    公开(公告)日：2012-09-27

    申请号：US13141885

    申请日：2009-12-23

    Applicant: Roland Fabis ,  Jan Petzel ,  Sabine Scheltinga

    Inventor： Roland Fabis ,  Jan Petzel ,  Sabine Scheltinga

    IPC: C07H1/06 ,  B01J41/08

    CPC classification number: C12N15/101

    Abstract: The present intimation relates to a method for purifying nucleic acids from a sample containing nucleic acids, the method comprising at least the following steps: a. bringing the sample containing nucleic acids into contact with a nucleic acid binding phase comprising protonatable groups, wherein the protonatable groups have a pKa value of 9 to 12; b. binding the nucleic acids to the nucleic acid phase at a pH (binding pH) that is at least one pH unit less than the pKa value of at least one of the protonatable groups; c. eluting the nucleic acids at a pH greater than the binding pH but at least one pH unit less than the pKa value of at least one of the protonatable groups. Also disclosed are corresponding kits and nucleic acid binding phases that can be used for purifying nucleic acids.

    Abstract translation: 本发明涉及从含有核酸的样品中纯化核酸的方法，所述方法至少包括以下步骤：a。 使含有核酸的样品与包含可质子基团的核酸结合相接触，其中可质子化基团的pKa值为9至12; b。 在至少一个pH值单位小于至少一个可质子化基团的pKa值的pH（结合pH）下将核酸结合到核酸相上; C。 在大于结合pH的pH下洗脱核酸，但是至少一个pH单位小于至少一个可质子化基团的pKa值。 还公开了可用于纯化核酸的相应试剂盒和核酸结合相。

    Process for the preparation of anion exchange resins of the poly(meth)acrylamide type
    73.
    发明授权
    Process for the preparation of anion exchange resins of the poly(meth)acrylamide type 失效
    制备聚（甲基）丙烯酰胺型阴离子交换树脂的方法

    公开(公告)号：US5414020A

    公开(公告)日：1995-05-09

    申请号：US160453

    申请日：1993-12-01

    Applicant: Harold Heller ,  Friedrich Werner ,  Alfred Mitschker ,  Axel Ingendoh

    Inventor： Harold Heller ,  Friedrich Werner ,  Alfred Mitschker ,  Axel Ingendoh

    IPC: B01J41/14 ,  C08F8/00 ,  C08F8/32 ,  B01J41/08 ,  B01J41/12

    CPC classification number: B01J41/14 ,  C08F8/32

    Abstract: According to the process according to the invention for the preparation of anion exchange resins of the poly(meth)acrylamide type by aminolysis of bead polymers of cross-linked poly(meth)acrylic acid esters with excess polyamine, subsequent separation of the anion exchange resins from the liquid phase containing the unreacted polyamine, washing the anion exchangers free of amine with water and recovering the polyamines from the washing waters, the liquid phase containing the unreacted polyamine and the polyamine-containing washing waters are immediately mixed with the bead polymer of cross-linked poly(meth)acrylic acid ester to be subjected to aminolysis, the amount of fresh polyamine necessary for the establishment of the desired polyamine excess is added to the mixture, the mixture thus obtained is distillatively dewatered until its water content has fallen to a value of

    Abstract translation: 根据本发明的制备聚（甲基）丙烯酰胺型阴离子交换树脂的方法，通过交联聚（甲基）丙烯酸酯与多余多胺的珠粒聚合物的分解，随后分离阴离子交换树脂 从含有未反应的多胺的液相中，用水洗涤不含胺的阴离子交换剂并从洗涤水中回收多胺，将含有未反应的多胺和含多胺洗涤水的液相立即与交叉的珠聚合物 连接的聚（甲基）丙烯酸酯进行氨解，将所需多胺过量所需的新鲜多胺的量加入混合物中，将得到的混合物进行蒸馏脱水，直到其含水量降至 值为<7重量％，并且在该蒸馏脱水后残留的混合物在该溶液中进行氨解 玛丽的样子

    Process for insolubilizing absorbed metal ions
    74.
    发明授权
    Process for insolubilizing absorbed metal ions 失效
    不溶解吸收金属离子的方法

    公开(公告)号：US5336704A

    公开(公告)日：1994-08-09

    申请号：US17598

    申请日：1993-02-16

    Applicant: Norman B. Rainer

    Inventor： Norman B. Rainer

    IPC: B01J41/08 ,  B01J45/00 ,  B01J47/00 ,  C08L11/00 ,  B01D15/00 ,  C08G69/26 ,  C08J5/20

    CPC classification number: B01J41/085 ,  B01J45/00 ,  B01J47/006 ,  B01J47/007 ,  C08J2479/00 ,  Y10T428/31721

    Abstract: Metal cations absorbed within a hydrogel chelation polymer are insolubilized for safe disposal by treating the polymer with a precipitative anion such as carbonate, aluminate, hydroxide, phosphate, silicate, or sulfide. Further insolubilization of the metal cations may be achieved by subsequent treatment of the polymer with a water-dilutable prepolymer composition such as phenol-formaldehyde resin, then heating to cause cross-linking of the prepolymer. The hydrogel chelation polymer, containing metal sulfide formed by virtue of the insolubilization process, is useful in abstracting from water metals whose sulfides are less soluble than the metal sulfide initially within the polymer.

    Abstract translation: 通过用沉淀阴离子例如碳酸盐，铝酸盐，氢氧化物，磷酸盐，硅酸盐或硫化物处理聚合物，在水凝胶螯合聚合物内吸收的金属阳离子不溶解以便安全处理。 金属阳离子的进一步不溶化可以通过用水可稀释的预聚物组合物如苯酚 - 甲醛树脂随后处理聚合物，然后加热以引起预聚物的交联来实现。 含有由于不溶化过程形成的金属硫化物的水凝胶螯合聚合物可用于从最初在聚合物内的金属硫化物的硫化物溶解度较低的水金属中提取。

    Method of stabilizing cation-exchange resins against oxidative degradation
    75.
    发明授权
    Method of stabilizing cation-exchange resins against oxidative degradation 失效
    稳定阳离子交换树脂对抗氧化降解的方法

    公开(公告)号：US5302623A

    公开(公告)日：1994-04-12

    申请号：US25006

    申请日：1993-03-02

    Applicant: Yog R. Dhingra ,  Paul R. VanTol

    Inventor： Yog R. Dhingra ,  Paul R. VanTol

    IPC: B01J39/20 ,  C08F12/00 ,  C08F212/04 ,  C08F212/34 ,  B01J39/18 ,  B01J41/08 ,  C08F8/36 ,  C08J5/20

    CPC classification number: B01J39/20 ,  Y10S526/909

    Abstract: Cation-exchange resins are provided which have improved resistance to copolymer degradation caused by oxidizing agents like molecular oxygen. The resins are prepared by incorporating a para-substituted styrenic monomer having an oxidation-stabilizing moiety in a position para to a polymerizable vinyl moiety. These cation-exchange resins can be used in separation processes for longer periods of time without detrimental increases in bed pressure drops, loss of operating capacity, or leaching of organic contaminants.

    Abstract translation: 提供了阳离子交换树脂，其具有改善的抗氧化剂如分子氧引起的共聚物降解性。 通过将具有氧化稳定性部分的对位取代的苯乙烯类单体并入可聚合的乙烯基部分的对位置来制备树脂。 这些阳离子交换树脂可以在分离过程中更长时间地使用，而不会有不利地增加床压降，运行能力损失或有机污染物的浸出。

    Production of powdered resin and the powdered resin so produced
    78.
    发明授权
    Production of powdered resin and the powdered resin so produced 失效
    生产粉末状树脂和如此生产的粉状树脂

    公开(公告)号：US4404346A

    公开(公告)日：1983-09-13

    申请号：US176732

    申请日：1980-08-05

    Applicant: Marico G. Pirotta ,  Giberto Garbagnati

    Inventor： Marico G. Pirotta ,  Giberto Garbagnati

    IPC: B01J47/00 ,  C08J3/12 ,  B01J41/08

    CPC classification number: B01J47/006 ,  C08J3/12 ,  C08J2327/04

    Abstract: Powdered synthetic polymeric resin is produced by swelling or shrinking resin particles by contact with water or an organic solvent to introduce strain within the particles and comminuting the swollen or shrunk particles by grinding them in a rotary attrition mill. This process is particularly useful for the production of powdered resins for oral consumption, for example powdered cholestyramine resin, since particle sizes such that 90% by weight and/or number is below 30 microns in average particle diameter in the wet swollen state may be achieved.

    Abstract translation: 粉碎的合成聚合物树脂通过与水或有机溶剂接触而使树脂颗粒膨胀或收缩来在颗粒内引入应变，并通过在旋转磨耗机中研磨它们来粉碎膨胀或收缩的颗粒。 该方法对于生产用于口服消费的粉末状树脂，例如粉状考来烯胺树脂是特别有用的，因为可以实现在湿膨胀状态下平均粒径为90重量％和/或数量小于30微米的粒径 。

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