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公开(公告)号：US07638617B2
公开(公告)日：2009-12-29
申请号：US12210618
申请日：2008-09-15
Applicant: Robert DiCosimo , Anna Panova , Daniel P. O'Keefe , Jeffery Scott Thompson , Mark S. Payne
Inventor： Robert DiCosimo , Anna Panova , Daniel P. O'Keefe , Jeffery Scott Thompson , Mark S. Payne
Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.
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82.发明授权公开(公告)号：US07528277B2
公开(公告)日：2009-05-05
申请号：US11315708
申请日：2005-12-21
Applicant: F. Glenn Gallagher , Robert DiCosimo
Inventor： F. Glenn Gallagher , Robert DiCosimo
CPC classification number: C07C67/08 , C07C51/09 , Y02P20/582 , C07C69/675 , C07C59/06 , C07C59/08 , C07C69/42 , C07C69/68 , C07C69/14 , C07C69/44 , C07C69/80 , C07C69/82 , C07C69/24
Abstract: The present invention relates to a method for obtaining a carboxylic acid ester from an aqueous solution of the corresponding carboxylic acid ammonium salt using alcoholysis. The recovered carboxylic acid ester may be subsequently hydrolyzed to produce the corresponding carboxylic acid.
Abstract translation: 本发明涉及使用醇解从相应的羧酸铵盐的水溶液中获得羧酸酯的方法。 随后将回收的羧酸酯水解以产生相应的羧酸。
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公开(公告)号：US20090111148A1
公开(公告)日：2009-04-30
申请号：US11930744
申请日：2007-10-31
Applicant: ROBERT DICOSIMO , Arie Ben-Bassat
Inventor： ROBERT DICOSIMO , Arie Ben-Bassat
CPC classification number: C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/588
Abstract: The present invention is directed to a process for improving the specific activity of a dehydrated enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid upon rehydration. In particular, a process is provided comprising pretreating an enzyme catalyst having nitrilase activity with glutaraldehyde, immobilizing the gutaraldehyde-preteated enzyme catalyst and chemically cross-linking the enzyme catalyst prior to dehydration. Upon rehydration, the enzyme catalyst exhibits improved specific nitrilase activity as compared to enzyme catalysts having nitrilase activity that are dehydrated and rehydrated without the processing described herein.
Abstract translation: 本发明涉及一种提高具有腈水解酶活性的脱水酶催化剂的比活性的方法，所述脱水酶催化剂在补液时将乙醇腈水解成乙醇酸。 特别地，提供了一种方法，其包括用戊二醛预处理具有腈水解酶活性的酶催化剂，在脱水之前固定化古巴醛预处理的酶催化剂和化学交联酶催化剂。 在再水合时，与不进行本文所述处理的具有脱水和再水合的腈水解酶活性的酶催化剂相比，酶催化剂表现出提高的特异性腈水解酶活性。
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公开(公告)号：US20090036716A1
公开(公告)日：2009-02-05
申请号：US11818353
申请日：2007-06-13
Applicant: Michael B. D'Amore , Leo Ernest Manzer , Edward S. Miller, JR. , Robert Dicosimo , Jeffrey P. Knapp
Inventor： Michael B. D'Amore , Leo Ernest Manzer , Edward S. Miller, JR. , Robert Dicosimo , Jeffrey P. Knapp
IPC: C07C41/01
Abstract: The present invention relates to a catalytic process for making dibutyl ethers using a reactant comprising 1-butanol and water. The dibutyl ethers so produced are useful in transportation fuels.
Abstract translation: 本发明涉及使用包含1-丁醇和水的反应物制备二丁醚的催化方法。 如此生产的二丁醚可用于运输燃料。
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85.发明申请公开(公告)号：US20080032371A1
公开(公告)日：2008-02-07
申请号：US11485575
申请日：2006-07-12
Applicant: Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Robert J. Trotman , Tina K. Van Dyk
Inventor： Robert Dicosimo , Sharon L. Haynie , Lixuan Lisa Huang , Fateme Sima Sariaslani , Robert J. Trotman , Tina K. Van Dyk
Abstract: TAL cell biocatalyst was immobilized in alginate cross-linked beads using low concentrations of glutaraldehyde. The biocatalyst beads have highly stable TAL activity and mechanical strength such that they withstand prolonged recycling in production of pHCA.
Abstract translation: 使用低浓度戊二醛将TAL细胞生物催化剂固定在藻酸盐交联珠粒中。 生物催化剂珠具有高度稳定的TAL活性和机械强度，使得它们在pHCA的生产中经受长时间的再循环。
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Patent Agency Ranking
公开(公告)号：US20070117192A1
公开(公告)日：2007-05-24
申请号：US11601980
申请日：2006-11-20
Applicant: Mark Payne , Robert DiCosimo , Daniel O'Keefe
Inventor： Mark Payne , Robert DiCosimo , Daniel O'Keefe
Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.
Abstract translation: 本发明涉及将3-羟基腈转化为3-羟基羧酸的具有改善的腈水解酶活性的腈水解酶突变体。 更具体地，使用易错PCR和定点诱变将Acidovorax facilis 72W（ATCC 55746）腈水解酶基因突变以产生具有改进的腈水解酶活性的腈水解酶，用于将3-羟基腈（例如3-羟基丁腈或3-羟基戊腈）转化为 相应的3-羟基羧酸。 还提供了使用这些改进的突变体产生3-羟基羧酸的方法。
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87.发明申请Enzymatic production of peracids from carboxylic acid ester substrates using non-heme haloperoxidases 审中-公开使用非血红素卤过氧化物酶从羧酸酯底物酶促生产过酸公开(公告)号：US20070082832A1
公开(公告)日：2007-04-12
申请号：US11524832
申请日：2006-09-21
Applicant: Robert DiCosimo , Mark Payne , Lorraine Wagner , John Gavagan
Inventor： Robert DiCosimo , Mark Payne , Lorraine Wagner , John Gavagan
IPC: C11D3/386
CPC classification number: C11D3/2093 , A01N37/16 , C11D1/667 , C11D3/38636 , C11D3/38654 , C11D3/39 , C11D3/3945 , C12P7/00 , C12P7/40 , A01N37/02 , A01N37/12 , A01N37/46 , A01N59/00 , A01N63/02 , A01N2300/00
Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted in situ with an inorganic peroxide in the presence of a non-heme haloperoxidase having perhydrolysis activity to produce peroxycarboxylic acids.
Abstract translation: 提供了从羧酸酯制备过氧羧酸的方法。 更具体地，在具有过水解活性的非血红素卤过氧化物酶的存在下，羧酸酯与无机过氧化物原位反应以产生过氧羧酸。
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公开(公告)号：US20070004017A1
公开(公告)日：2007-01-04
申请号：US11516408
申请日：2006-09-05
Applicant: Michael Bramucci , Robert Dicosimo , Robert Fallon , John Gavagan , Frank Herkes , Lech Wilczek
Inventor： Michael Bramucci , Robert Dicosimo , Robert Fallon , John Gavagan , Frank Herkes , Lech Wilczek
Abstract: This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.
Abstract translation: 本发明涉及一种由3-羟基腈制备3-羟基羧酸的方法。 更具体地，使用酶催化剂1）腈水合酶活性和酰胺酶活性或2）微生物细胞的腈水解酶活性，将3-羟基戊腈以高产率转化为3-羟基戊酸。 3-羟基戊酸用于制备高支化共聚酯中的ε-己内酯的替代物。
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公开(公告)号：US07148051B2
公开(公告)日：2006-12-12
申请号：US10919182
申请日：2004-08-16
Applicant: Mark S. Payne , Robert DiCosimo , Daniel P. O'Keefe
Inventor： Mark S. Payne , Robert DiCosimo , Daniel P. O'Keefe
Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.
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公开(公告)号：US07091011B2
公开(公告)日：2006-08-15
申请号：US10378202
申请日：2003-03-03
Applicant: Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
Inventor： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
Abstract: This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.
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