MOUSE CELL LINE AUTHENTICATION
    82.
    发明申请
    MOUSE CELL LINE AUTHENTICATION 有权
    鼠标细胞线认证

    公开(公告)号:US20140066322A1

    公开(公告)日:2014-03-06

    申请号:US13935285

    申请日:2013-07-03

    CPC classification number: C12Q1/6881 C12Q2600/156 C12Q2600/16

    Abstract: A multiplex polymerase chain reaction assay that targets nine tetranucleotide short tandem repeat (STR) markers in the mouse genome. Unique profiles were obtained from seventy-two mouse samples that were used to determine the allele distribution for each STR marker. Correlations between allele fragment length and repeat number were determined with DNA Sanger sequencing. Genotypes for L929 and NIH3T3 cell lines were shown to be stable with increasing passage numbers as there were no significant differences in fragment length with samples of low passage when compared to high passage samples. In order to detect cell line contaminants, primers for two human STR markers were incorporated into the multiplex assay to facilitate detection of human and African green monkey DNA. This multiplex assay is the first of its kind to provide a unique STR profile for each individual mouse sample and can be used to authenticate mouse cell lines.

    Abstract translation: 在小鼠基因组中靶向九个四核苷酸短串联重复(STR)标记的多重聚合酶链反应测定。 从72个小鼠样品中获得独特的分布,用于确定每个STR标记的等位基因分布。 用DNA Sanger测序法测定等位基因片段长度和重复序列之间的相关性。 显示L929和NIH3T3细胞系的基因型随着通道数的增加而稳定,因为与高通道样品相比,片段长度与低通道样品无显着性差异。 为了检测细胞系污染物,将两个人STR标记的引物并入多重测定中,以便于检测人和非洲绿猴DNA。 该多重测定法是第一种为每个单独的小鼠样品提供独特的STR特征,可用于认证小鼠细胞系。

    Superconducting switch
    86.
    发明授权

    公开(公告)号:US12245524B2

    公开(公告)日:2025-03-04

    申请号:US18632077

    申请日:2024-04-10

    Abstract: An example electric circuit includes a superconductor component having a first terminal at a first end and a second terminal at a second end. The superconductor component includes a first portion coupled to the first terminal, a second portion coupled to the second terminal, and a third portion coupling the first portion and the second portion. The third portion has a curved shape such that the first portion of the superconductor component is proximate to, and thermally coupled to, the second portion of the superconductor component. The example electric circuit further includes a coupling component coupled to the third portion of the superconductor component, and a gate component configured to generate a resistive heat that exceeds a superconducting threshold temperature of the superconductor component, where the gate component is separated from the superconductor component by the coupling component.

    DNA nanotechnology-based biomarker measurement platform

    公开(公告)号:US12195787B2

    公开(公告)日:2025-01-14

    申请号:US17360008

    申请日:2021-06-28

    Abstract: A biomarker signal amplifier amplifies chemical analyte binding and includes: a surface strand disposed on an analysis substrate and including an exchange region; a particle strand hybridized to the surface strand in an absence of a chemical analyte that preferentially hybridizes to the exchange region as compared with the particle strand, and the particle strand is dissociated from the surface strand when the surface strand is in a presence of the chemical analyte; and a reporter particle attached to the particle strand and disposed proximate to the analysis substrate when the particle strand is hybridized to the surface strand in absence of the chemical analyte and that changes the electrical potential of the analysis substrate depending on whether the particle strand is hybridized to the surface strand.

    System and method for measurment of magnetic nanoparticles

    公开(公告)号:US12188900B2

    公开(公告)日:2025-01-07

    申请号:US17669992

    申请日:2022-02-11

    Abstract: Embodiments of the present invention relate to a system and method for performing temperature-dependent measurements of a magnetic nanoparticle sample. The system includes high frequency coils and sample temperature tunable assembly to determine the specific relaxation process for magnetic nanoparticle sample using both time and frequency domain techniques. During the temperature-dependent measurements of a magnetic nanoparticle sample, system in accordance with embodiments of the present invention resolve the nanoparticle dynamics using a temperature-tunable dual mode, AC susceptibility and magnetic relaxometry, to cover a broad range of frequencies and time scales. Other operational modes of the invention can drive the nanoparticles with arbitrary waveforms (sinusoidal, sum of sinusoids, or repeated pulses) to elicit and measure tailored response behavior from the magnetic nanoparticle sample.

    CHIP TWEEZERS
    89.
    发明申请

    公开(公告)号:US20240420989A1

    公开(公告)日:2024-12-19

    申请号:US18745476

    申请日:2024-06-17

    Abstract: Chip tweezers for handling microchips include a body portion having a pair of arms coupled together at a proximal end and extending toward a distal end, wherein the arms are moveable towards and away from each other; and a head portion located at the distal end, the head portion including a pair of V-grooves disposed parallel to each other with open sides facing each other, each rigidly connected to a respective arm of the pair of arms.

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