公开(公告)号：US20240148811A1

公开(公告)日：2024-05-09

申请号：US18479876

申请日：2023-10-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Carl Andre

IPC: A61K36/31 ,  C12Q1/6895

CPC classification number: A61K36/31 ,  C12Q1/6895 ,  C12N15/8247

Abstract: The present invention relates generally to the field of molecular biology and concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. The present invention also relates to methods for the manufacture of oil, fatty acid- or lipids-containing compositions, and to such oils and lipids as such.

公开(公告)号：US11957098B2

公开(公告)日：2024-04-16

申请号：US17046989

申请日：2019-04-11

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Xinmin Deng ,  Kristin Gray ,  Jakir Hasan ,  Keith Horton

IPC: C12N15/82 ,  A01H1/00 ,  A01H5/10 ,  A01H6/20

CPC classification number: A01H1/105 ,  A01H5/10 ,  A01H6/202

Abstract: Various embodiments disclosed relate to increasing the proportion of omega-3 fatty acid in seed oil produced by a plurality of Brassica plants, such as canola, transgenically modified to produce seed oil comprising at least one of EPA, DHA and DPA. Transgenic Brassica plants, such as transgenic canola, are subjected to an environment which has an average daily day-night temperature difference of at least 13° C. during the transgenic plant's period of seed maturation. The seed oil is at least 5 wt % EPA. The seed oil is at least 1 wt % DPA. The seed oil is at least 0.2 wt % DHA. The seed oil is at least 5.2 wt % a mixture of EPA and DHA. The seed oil is at least 6 wt % long chain omega-3 fatty acids.

公开(公告)号：US20230183728A1

公开(公告)日：2023-06-15

申请号：US17273858

申请日：2019-09-06

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Hui Yang ,  Carl Andre ,  Patricia Vrinten

IPC: C12N15/82 ,  C12N9/12 ,  C07K16/40 ,  A23D9/00 ,  A23L33/115

CPC classification number: C12N15/8247 ,  A23D9/00 ,  A23L33/115 ,  C07K16/40 ,  C12N9/1288 ,  C12N15/8218 ,  C12Y207/08002 ,  A23V2002/00

Abstract: The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

公开(公告)号：US11613761B1

公开(公告)日：2023-03-28

申请号：US15526550

申请日：2015-11-13

Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  BIORIGINAL FOOD & SCIENCE CORPORATION

Inventor： Toralf Senger ,  Laurent Marty ,  Irene Kunze ,  Heiko Haertel ,  Steven Craig Bremmer ,  Steven D. Breazeale ,  Joerg Bauer ,  Patricia Vrinten ,  Sten Stymne ,  Jenny Lindberg Yilmaz ,  John A. McElver ,  Dietrich Rein ,  Carl Andre

IPC: C12N15/82 ,  C12N9/02

Abstract: T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

公开(公告)号：US11608499B2

公开(公告)日：2023-03-21

申请号：US16097300

申请日：2017-04-20

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Leendert W. Neuteboom ,  John A. McElver ,  Rosa De Pinho Barroco ,  Chris De Wilde ,  Max Fabian Felle

IPC: C12N15/11 ,  C12N15/10 ,  C12N15/70 ,  C12N15/75 ,  C12N15/82 ,  C12N15/85 ,  C12N15/90 ,  C12N15/81

Abstract: Methods for modification of target nucleic acids. The method involves a construct in which guide RNA is covalently linked to donor RNA (fusion NA) to be introduced into the target nucleic acid by homologous recombination and is based on the introduction of a nuclease, e.g. CRISPR or TALEN, into the cell containing the target nucleic acid. The fusion NA may be introduced as a DNA vector.

公开(公告)号：US20230030539A1

公开(公告)日：2023-02-02

申请号：US17782290

申请日：2020-12-04

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Elie Fux ,  Sandra Gonzalez Maldonado ,  Michael Herold

IPC: G16B40/10 ,  G01N33/68 ,  G16H50/20 ,  G16H10/40

Abstract: The invention relates to a method of analyzing the metabolic content of a biological sample comprising: i) providing one or more samples of extracted metabolites from the biological sample; ii) performing a chromatography coupled mass spectrometry analysis of the extracted metabolites to generate a full raw data set for full scan ions; iii) generating a full data cluster set from the full raw data set obtained in step ii) by grouping full scan ions according to isotope and adduct values; iv) performing a tandem mass spectrometry analysis of the extracted metabolites with a plurality of mass selection windows to generate a raw SWATH® data set for fragment ions; v) generating a SWATH® data cluster set from the raw SWATH® data set obtained in step iv) by grouping fragment ions according to retention time and mass values; vi) aligning the SWATH® data cluster set with the full data cluster set to generate characteristic profile for each extracted metabolite; vii) comparing the data using R characteristic profile of each extracted metabolite obtained in step vi) with a reference library of characteristic profiles of metabolites to provide the metabolic content of the biological sample.

公开(公告)号：US11503837B2

公开(公告)日：2022-11-22

申请号：US16300358

申请日：2017-05-12

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Carl Andre ,  Anthony J. Cavender

IPC: A23D9/02 ,  C12N15/82 ,  A01D45/30

Abstract: The present invention relates to the field of producing particular metabolites of interest by engineered crop plants such as transgenic crop plants. Provided are methods that are easily applicable by farmers to determine when the metabolites of interest hake reached an optimal content in the plant. These methods also help to facilitate decisions about the timeframe for preparing harvest or harvesting the engineered crop plant.

公开(公告)号：US11484560B2

公开(公告)日：2022-11-01

申请号：US15525463

申请日：2015-11-13

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Heiko A. Haertel

IPC: C12N15/82 ,  A61K36/31 ,  C12Q1/6895 ,  C12N9/02 ,  C12N9/00 ,  A61K31/202

Abstract: Assay method, comprising providing a plant capable of expressing a delta-12 desaturase, wherein said delta-12 desaturase has at least 50% total amino acid sequence identity to at least one of the sequences SEQ ID NO. 328 to 336, and/or at least 59% total amino acid sequence similarity to at least one of the sequences SEQ ID NO. 328 to 336, and wherein the plant is also capable of expressing at least one or more enzymes of unsaturated fatty acid metabolism, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is supposedly connected to a plant metabolic property, growing the plant, and measuring said plant metabolic property for said plant.

公开(公告)号：US11472852B2

公开(公告)日：2022-10-18

申请号：US16276616

申请日：2019-02-15

Applicant: Monsanto Technology LLC ,  BASF Plant Science LP ,  BASF Plant Science Company GmbH

Inventor： Leonardo Alves-Junior ,  Wesley B. Bruce ,  Charles R. Dietrich ,  Natalia Ivleva ,  Kian Kiani ,  Ryan Rapp ,  Thomas L. Slewinski

IPC: C12N15/82 ,  C07K14/415 ,  C12N9/02 ,  C12N15/52

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, increased single kernel weight, increased ear fresh weight, increased number of florets, and mitigated flowering delay. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

公开(公告)号：US20220186249A1

公开(公告)日：2022-06-16

申请号：US17532695

申请日：2021-11-22

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Holger Schultheiss ,  Tobias Mentzel ,  Ralf Flachmann

IPC: C12N15/82 ,  C12N9/12

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an RLK2 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an RLK2 protein.