公开(公告)号：US20210389245A1

公开(公告)日：2021-12-16

申请号：US17282807

申请日：2019-10-04

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Alan Lloyd Weeks ,  Valerie Villareal ,  Patrick Murphy ,  Eric Anthony Robertson ,  Deirdre Ellen Day ,  Matthew Bernard Dean ,  Todd Glendon Campbell ,  Brian Christopher Burke ,  Tyler S. Smith ,  Thomas Christopher Hartner ,  Stanley Owen Thompson ,  Nhat Nam Trinh ,  David Carpenter ,  Gregory J. Kintz ,  Paul Koh ,  David Jennings Dostal ,  Kevin Doherty ,  Joel F. Jensen ,  William Law ,  Russell C. Mead, Jr. ,  J. Efraín Alcorta

IPC: G01N21/64 ,  B01L3/00

Abstract: The present disclosure is drawn to devices and systems for target detection in samples, particularly allergen detection in food samples. The allergen detection system includes a sampler, a disposable analysis cartridge and a detection device with an optimized optical system. The allergen detection utilizes nucleic acid molecules as detection agents and detection probes.

公开(公告)号：US20210311032A1

公开(公告)日：2021-10-07

申请号：US16971701

申请日：2019-02-21

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Alan Lloyd Weeks ,  Valerie Villareal ,  Patrick Murphy ,  Eric Anthony Robertson ,  David Carpenter ,  Deirdre Ellen Day ,  Matthew Bernard Dean ,  Todd Glendon Campbell ,  Gregory J. Kintz ,  Paul Koh ,  David Jennings Dostal ,  Kevin Doherty ,  Joel F. Jensen ,  William Law ,  Russell C. Mead, Jr. ,  J. Efraín Alcorta

IPC: G01N33/53 ,  G01N33/543 ,  C12Q1/6816 ,  G01N33/02

Abstract: The present invention is drawn to devices and systems for allergen detection in food samples. The allergen detection system includes a disposable analysis cartridge and a detection device with an optimized optical system.

公开(公告)号：US20210109076A1

公开(公告)日：2021-04-15

申请号：US17128270

申请日：2020-12-21

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown ,  Sarah Stidham ,  Valerie Villareal ,  Adam J. Young ,  Joshua Glenn Anthony ,  Patrick Murphy ,  John H. Kepler ,  Adam Jacobs

IPC: G01N33/02 ,  C12M1/34 ,  G01N1/08 ,  C12N15/115 ,  G01N1/14 ,  G01N1/28 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are provided as detection agents. SPNs have a single open structure, and are labeled with a fluorophore. Changes in fluorescence polarization of SPNs upon the binding of allergens are measured to calculate the allergen content in a sample.

公开(公告)号：US10466246B2

公开(公告)日：2019-11-05

申请号：US15569808

申请日：2016-04-26

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen

IPC: G01N33/58 ,  G01N33/68 ,  A61K38/00 ,  C12M1/34 ,  G01N33/53 ,  G01N33/542 ,  A61P37/00

Abstract: The present invention is drawn to nucleic acid aptamer based signaling polynucleotides (SPNs) for allergen detection in samples. Disclosed herein include compositions, compounds, assays and methods of using said SPNs to detect one or more allergens in a sample, particularly food allergens in a food product.

公开(公告)号：US11034963B2

公开(公告)日：2021-06-15

申请号：US16347935

申请日：2017-11-08

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Valerie Villareal ,  Sarah Stidham ,  Morris Nehama

IPC: C12N15/115 ,  C12N15/10 ,  G01N33/02 ,  G01N33/533 ,  C12Q1/6816

Abstract: The present application discloses nucleic acid aptamer based signaling polynucleotides (SPNs) that specifically bind an allergen protein. Provided in the present invention include aptamers, SPNs, SPN-complement complexes, magnetic particle conjugates, DNA printed glass slides and detection agents, and detection methods using the same for detecting the presence, or absence of an allergen protein in a food sample.

公开(公告)号：US20180188139A1

公开(公告)日：2018-07-05

申请号：US15736801

申请日：2016-06-20

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown

IPC: G01N1/28 ,  G01N33/543 ,  G01N33/53 ,  G01N33/68 ,  G01N1/40 ,  G01N33/02 ,  G01N33/00

CPC classification number: G01N1/286 ,  G01N1/4044 ,  G01N33/0098 ,  G01N33/02 ,  G01N33/025 ,  G01N33/5304 ,  G01N33/54366 ,  G01N33/6854 ,  G01N2001/2866

Abstract: The present invention relates to assays and methods for detecting the presence, absence and/or amount of an allergen (e.g., a food allergen) in a sample. The current assays and methods improve sampling processes, increase detection accuracy and sensitivity, and reduce the detection time.

公开(公告)号：US20230324384A1

公开(公告)日：2023-10-12

申请号：US18022591

申请日：2021-09-09

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen

IPC: G01N33/569 ,  G01N33/58 ,  G01N33/50 ,  G01N21/64

CPC classification number: G01N33/56972 ,  G01N33/582 ,  G01N33/5047 ,  G01N21/6428 ,  G01N2333/70596 ,  G01N2800/24 ,  G01N2800/52 ,  G01N2021/6439

Abstract: The present disclosure relates to aptamer mediated BAT for diagnosing and/or prognosing an individual's allergic reaction to an allergen.

公开(公告)号：US11591557B2

公开(公告)日：2023-02-28

申请号：US16487450

申请日：2018-02-21

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Gregory J. Kintz ,  Adam J. Young ,  Patrick Murphy ,  Joshua Glenn Anthony ,  Paul Fleming ,  Brett Gorham ,  Ryan Griswold ,  Bruce Richardson

IPC: C12M3/06 ,  C12M1/32 ,  G01J3/42 ,  G01J3/44 ,  G01N21/51 ,  G01N21/53 ,  G01N21/64 ,  G01N33/02 ,  G01N21/17

Abstract: The present invention is drawn to devices and systems for allergen detection in a sample. The allergen detection system includes a sampler, a disposable analysis cartridge and a detection device with an optimized optical system. In some embodiments, the allergen detection utilizes aptamer nucleic acid molecules as detection agents. In some embodiments, the nucleic acids are conjugated to magnetic beads or solid surfaces such as glasses, microwells and microchips.

公开(公告)号：US20220073911A1

公开(公告)日：2022-03-10

申请号：US17265550

申请日：2019-08-02

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Sarah Elizabeth Stidham ,  Olivia Jean Alley

IPC: C12N15/11 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N33/02

Abstract: The present disclosure relates to methods for identifying aptamers against allergen proteins and signaling polynucleotides (SPNs) for allergen detection. The screening method of the present disclosure combines several positive SELEX selections, and on-chip positive and counter selections to identify aptamer sequences that are preferentially bind to target proteins when competing with short complementary sequences.

公开(公告)号：US10908139B2

公开(公告)日：2021-02-02

申请号：US16084996

申请日：2017-03-10

Applicant: DOTS Technology Corp.

Inventor： Adi Gilboa-Geffen ,  Renuka Babu Brown ,  Sarah Stidham ,  Valerie Villareal ,  Adam J. Young ,  Joshua Glenn Anthony ,  Patrick Murphy ,  John H. Kepler ,  Adam Jacobs

IPC: B01L3/00 ,  B01L1/00 ,  G01N33/02 ,  C12M1/34 ,  G01N1/08 ,  C12N15/115 ,  G01N1/14 ,  G01N1/28 ,  G01N33/53 ,  G01N33/58

Abstract: The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are provided as detection agents. SPNs have a single open structure, and are labeled with a fluorophore. Changes in fluorescence polarization of SPNs upon the binding of allergens are measured to calculate the allergen content in a sample.