公开(公告)号：US20230123594A1

公开(公告)日：2023-04-20

申请号：US17965207

申请日：2022-10-13

Applicant: Fundamental Solutions Corporation

Inventor： Joseph D. Kittle, JR.

IPC: C12Q1/18 ,  G01N21/64 ,  G01N33/569

Abstract: A method for determining the susceptibility of a bacteria to an antibiotic, comprising transferring one portion of a sample containing living bacterial cells into a bacterial growth medium to create a control sample; transferring another portion of the sample into a bacterial growth medium to which a predetermined amount of antibiotic or predetermined amount of a library of antibiotics has been added to create a test sample; adding an alkyne-modified non-canonical amino acid to both the control sample and test sample during bacterial growth, wherein the alkyne-modified non-canonical amino acid incorporates into surface proteins, internal proteins, or both; reacting the alkyne-containing proteins with an azide-modified detection molecule using click-chemistry to label the cells; detecting the labeled cells using a method that generates a detectable signal; and comparing the signal generated by the control sample to the signal generated by the test sample, wherein a decrease in detectable signal between the control sample and the test sample is indicative of susceptibility of the living bacteria to the predetermined antibiotic or predetermined library of antibiotics.

公开(公告)号：US10739347B2

公开(公告)日：2020-08-11

申请号：US16105303

申请日：2018-08-20

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： M. Russell Williams ,  Joel Lwande ,  Shengwen Liang ,  Kyle McQuaid

IPC: G01N33/58 ,  G01N33/542 ,  C12N9/02

Abstract: A bioassay for the detection of multiple target analytes that includes living immunocytes of a plurality of predetermined types; a signal-generating reporter within each immunocyte that is responsive to predetermined changes occurring within the immunocyte and that includes a bioluminescent protein linked to a fluorescent protein; a signal transduction pathway associated with each signal-generating reporter that is operative to induce the predetermined changes within each immunocyte; a detector element associated with the signal transduction pathway that is operative to trigger the signal transduction pathway; an analyte binding element associated with the detector element that is specific to both the detector element and a target analyte; and wherein upon the binding of an analyte binding element to which a target analyte is also bound to the detector element, the detector element triggers the signal transduction pathway causing the predetermined changes to occur within the living biological cell, thereby causing the signal-generating reporter to generate a detectable signal.

公开(公告)号：US20180180608A1

公开(公告)日：2018-06-28

申请号：US15848104

申请日：2017-12-20

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Thomas J. ZUPANCIC ,  Joseph D. KITTLE ,  Lingchun ZENG ,  Srikanth VEDAMOORTHY ,  Richard S. BRODY ,  Marvin R. WILLIAMS

IPC: G01N33/554 ,  G01N33/569 ,  G01N33/74 ,  G01N33/564

CPC classification number: G01N33/554 ,  C12N5/00 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/564 ,  G01N33/56916 ,  G01N33/74

Abstract: A biosensor system for the detection of target analytes that includes a living biological cell of a predetermined type; a signal-generating reporter associated with the living biological cell; a signal transduction pathway or other activator mechanism or means associated with the signal-generating reporter; a universal detector element associated with the activator mechanism; and an analyte binding element associated with the universal detector element, wherein the analyte binding element is specific to both the universal detector element and a target analyte.

公开(公告)号：US20170306422A1

公开(公告)日：2017-10-26

申请号：US15642800

申请日：2017-07-06

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Thomas J. ZUPANCIC ,  Lingchun ZENG ,  Srikanth VEDAMOORTHY ,  Joel S. LWANDE ,  Joseph D. KITTLE ,  Min MO

IPC: C12Q1/68 ,  C07K14/725 ,  C07K14/735 ,  C07K16/12 ,  G01N33/569 ,  C07K14/315 ,  G01N33/536 ,  C07K14/36 ,  G01N33/50

CPC classification number: C12Q1/6897 ,  C07K14/315 ,  C07K14/36 ,  C07K14/7051 ,  C07K14/70535 ,  C07K16/12 ,  C07K2317/52 ,  C07K2319/22 ,  C07K2319/60 ,  G01N33/5047 ,  G01N33/536 ,  G01N33/56916 ,  G01N2333/245

Abstract: A system, device, and method for rapid detection of analytes that includes a living, engineered biosensor cell that is typically a component of the mammalian immune system; a reporter protein that is engineered into and expressed by the living, engineered biosensor cell, wherein the reporter protein emits a detectable signal in response to certain predetermined changes in the cytosol of the living, engineered cell; a signal transduction pathway expressed by the living, engineered biosensor cell, wherein the signal transduction pathway controls a biological process within the cytosol of the living, engineered biosensor cell, and wherein the biochemical process, when it occurs, causes the reporter protein to emit a detectable signal; at least one type of detector molecule that is adapted to bind to a specific analyte; at least one analyte that binds to the detector molecule that is specific to that analyte; a plurality of non-antibody signal transducing elements that are either expressed by the living, engineered biosensor cell or that actively bind to a receptor or a receptor component expressed by the living, engineered biosensor cell, wherein each signal transducing element is adapted to receive a detector molecule.

公开(公告)号：US09752199B2

公开(公告)日：2017-09-05

申请号：US15086769

申请日：2016-03-31

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Thomas J. Zupancic ,  Lingchun Zeng ,  Srikanth Vedamoorthy ,  Joel S. Lwande ,  Joesph D. Kittle ,  Min Mo

IPC: C07K16/12 ,  C12Q1/68 ,  C07K14/315 ,  C07K14/725 ,  C07K14/735 ,  C07K14/36 ,  G01N33/536 ,  G01N33/569 ,  G01N33/50

CPC classification number: C12Q1/6897 ,  C07K14/315 ,  C07K14/36 ,  C07K14/7051 ,  C07K14/70535 ,  C07K16/12 ,  C07K2317/52 ,  C07K2319/22 ,  C07K2319/60 ,  G01N33/5047 ,  G01N33/536 ,  G01N33/56916 ,  G01N2333/245

Abstract: A system, device, and method for rapid detection of analytes that includes a living, engineered biosensor cell that is typically a component of the mammalian immune system; a reporter protein that is engineered into and expressed by the living, engineered biosensor cell, wherein the reporter protein emits a detectable signal in response to certain predetermined changes in the cytosol of the living, engineered cell; a signal transduction pathway expressed by the living, engineered biosensor cell, wherein the signal transduction pathway controls a biological process within the cytosol of the living, engineered biosensor cell, and wherein the biochemical process, when it occurs, causes the reporter protein to emit a detectable signal; at least one type of detector molecule that is adapted to bind to a specific analyte; at least one analyte that binds to the detector molecule that is specific to that analyte; a plurality of non-antibody signal transducing elements that are either expressed by the living, engineered biosensor cell or that actively bind to a receptor or a receptor component expressed by the living, engineered biosensor cell, wherein each signal transducing element is adapted to receive a detector molecule.

公开(公告)号：US20190284255A1

公开(公告)日：2019-09-19

申请号：US16353337

申请日：2019-03-14

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Joseph D. Kittle ,  Joel S. Lwande ,  Yuanyuan Tang ,  Shengwen Liang

IPC: C07K14/725 ,  C07K14/36 ,  C07K14/47 ,  C07K14/735 ,  C12N5/0783 ,  A61K35/17

Abstract: A programmable receptor complex expressed by an immunocyte, wherein the programmable receptor complex includes a plurality of native or endogenously-expressed receptor subunits, wherein at least one of the plurality of native or endogenously-expressed receptor subunits has been engineered or modified to include an FcγRI receptor component or a biotin-binding component, and wherein the FcγRI receptor component or biotin-binding component is operative to bind to a target detector molecule that binds to or otherwise interacts with a predetermined target.

公开(公告)号：US20180066300A1

公开(公告)日：2018-03-08

申请号：US15790499

申请日：2017-10-23

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Marvin R. WILLIAMS ,  Charles MCBRAIRTY ,  Andrew WEIMAN ,  Daniel W. PFAUTZ ,  Thomas J. ZUPANCIC

IPC: C12Q1/04 ,  G01N21/76 ,  G01N33/12 ,  B01L3/00 ,  G01N17/00

CPC classification number: C12Q1/04 ,  B01L3/50 ,  B01L3/502 ,  B01L3/527 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2300/168 ,  B01L2400/0478 ,  G01N17/002 ,  G01N21/63 ,  G01N21/763 ,  G01N33/02 ,  G01N33/025 ,  G01N33/12 ,  G01N33/56916 ,  G01N2021/0325 ,  G01N2201/0221 ,  G01N2333/245

Abstract: A method for enhancing the performance of a reaction chamber for use in a sample analysis system that includes providing a reaction chamber, wherein the reaction chamber has a predetermined geometry, wherein the reaction chamber has light collecting properties, and wherein the reaction chamber has an inner surface; and modifying the inner surface of the reaction chamber to include a predetermined degree of reflectivity, wherein the predetermined degree of reflectivity enhances the light collecting properties of the reaction chamber.

公开(公告)号：US09850547B2

公开(公告)日：2017-12-26

申请号：US15642867

申请日：2017-07-06

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Thomas J. Zupancic ,  Lingchun Zeng ,  Srikanth Vedamoorthy ,  Joel S. Lwande ,  Joseph D. Kittle ,  Min Mo

IPC: G01N33/53 ,  C12Q1/68 ,  C07K14/735 ,  G01N33/536 ,  G01N33/569 ,  C07K14/725 ,  C07K14/36 ,  C07K14/315 ,  C07K16/12 ,  G01N33/50

CPC classification number: C12Q1/6897 ,  C07K14/315 ,  C07K14/36 ,  C07K14/7051 ,  C07K14/70535 ,  C07K16/12 ,  C07K2317/52 ,  C07K2319/22 ,  C07K2319/60 ,  G01N33/5047 ,  G01N33/536 ,  G01N33/56916 ,  G01N2333/245

Abstract: A system, device, and method for rapid detection of analytes that includes a living, engineered biosensor cell that is typically a component of the mammalian immune system; a reporter protein that is engineered into and expressed by the living, engineered biosensor cell, wherein the reporter protein emits a detectable signal in response to certain predetermined changes in the cytosol of the living, engineered cell; a signal transduction pathway expressed by the living, engineered biosensor cell, wherein the signal transduction pathway controls a biological process within the cytosol of the living, engineered biosensor cell, and wherein the biochemical process, when it occurs, causes the reporter protein to emit a detectable signal; at least one type of detector molecule that is adapted to bind to a specific analyte; at least one analyte that binds to the detector molecule that is specific to that analyte; a plurality of non-antibody signal transducing elements that are either expressed by the living, engineered biosensor cell or that actively bind to a receptor or a receptor component expressed by the living, engineered biosensor cell, wherein each signal transducing element is adapted to receive a detector molecule.

公开(公告)号：US09850546B2

公开(公告)日：2017-12-26

申请号：US15642800

申请日：2017-07-06

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Thomas J. Zupancic ,  Lingchun Zeng ,  Srikanth Vedamoorthy ,  Joel S. Lwande ,  Joseph D. Kittle ,  Min Mo

IPC: G01N33/53 ,  C12Q1/68 ,  C07K14/735 ,  G01N33/536 ,  G01N33/569 ,  C07K14/725 ,  C07K14/36 ,  C07K14/315 ,  C07K16/12 ,  G01N33/50

CPC classification number: C12Q1/6897 ,  C07K14/315 ,  C07K14/36 ,  C07K14/7051 ,  C07K14/70535 ,  C07K16/12 ,  C07K2317/52 ,  C07K2319/22 ,  C07K2319/60 ,  G01N33/5047 ,  G01N33/536 ,  G01N33/56916 ,  G01N2333/245

Abstract: A system, device, and method for rapid detection of analytes that includes a living, engineered biosensor cell that is typically a component of the mammalian immune system; a reporter protein that is engineered into and expressed by the living, engineered biosensor cell, wherein the reporter protein emits a detectable signal in response to certain predetermined changes in the cytosol of the living, engineered cell; a signal transduction pathway expressed by the living, engineered biosensor cell, wherein the signal transduction pathway controls a biological process within the cytosol of the living, engineered biosensor cell, and wherein the biochemical process, when it occurs, causes the reporter protein to emit a detectable signal; at least one type of detector molecule that is adapted to bind to a specific analyte; at least one analyte that binds to the detector molecule that is specific to that analyte; a plurality of non-antibody signal transducing elements that are either expressed by the living, engineered biosensor cell or that actively bind to a receptor or a receptor component expressed by the living, engineered biosensor cell, wherein each signal transducing element is adapted to receive a detector molecule.

公开(公告)号：US20150197784A1

公开(公告)日：2015-07-16

申请号：US14669970

申请日：2015-03-26

Applicant: FUNDAMENTAL SOLUTIONS CORPORATION

Inventor： Marvin R. WILLIAMS ,  Charles MCBRAIRTY ,  Daniel W. PFAUTZ ,  Thomas J. ZUPANCIC ,  Lingchun ZENG ,  Andrew WEIMAN ,  Richard S. BRODY ,  Joseph D. KITTLE ,  Anthony TRUSCOTT ,  Robert BARANOWSKI

IPC: C12Q1/10

CPC classification number: C12Q1/10 ,  G01N21/75 ,  G01N21/763 ,  G01N33/12 ,  G01N33/56916 ,  G01N2201/0221

Abstract: A system for use in rapid sample analysis that includes a biosensor reagent, wherein the biosensor reagent includes living biological cells; a reservoir card, wherein the reservoir card stores the biosensor reagent; and a test cartridge base, wherein the test cartridge base is configured to accept the reservoir card, and wherein the test cartridge base further includes a reaction chamber having a central axis, wherein the reaction chamber has the shape of a revolved half ellipse; and an inlet channel connected to the reaction chamber, wherein the inlet channel is positioned above the reaction chamber at an angle of 15-60 degrees above the horizontal, and wherein the inlet channel is offset from the central axis of the reaction chamber.

Abstract translation: 一种用于快速样品分析的系统，其包括生物传感器试剂，其中所述生物传感器试剂包括活的生物细胞; 储存卡，其中储存卡存储生物传感器试剂; 以及测试盒底座，其中所述测试盒底座构造成接纳所述储存卡，并且其中所述测试盒底座还包括具有中心轴线的反应室，其中所述反应室具有旋转的半椭圆形状; 以及连接到所述反应室的入口通道，其中所述入口通道以所述水平面上方15-60度的角度位于所述反应室上方，并且其中所述入口通道偏离所述反应室的中心轴线。