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    METHODS FOR IMPROVED HOMOLOGOUS RECOMBINATION AND COMPOSITIONS THEREOF
    2.
    发明申请
    METHODS FOR IMPROVED HOMOLOGOUS RECOMBINATION AND COMPOSITIONS THEREOF 有权

    公开(公告)号：US20250154531A1

    公开(公告)日：2025-05-15

    申请号：US18924629

    申请日：2024-10-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chi-Hsuan CHANG ,  Xiquan LIANG

    IPC: C12N15/87 ,  C12N5/078 ,  C12N5/0783 ,  C12N9/22 ,  C12N15/11

    Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

    ASEPTIC TUBING DISCONNECT ASSEMBLY
    4.
    发明申请
    ASEPTIC TUBING DISCONNECT ASSEMBLY 有权

    公开(公告)号：US20250146605A1

    公开(公告)日：2025-05-08

    申请号：US18933336

    申请日：2024-10-31

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kim-Xuan Dinh Nguyen ,  Rick W. Hendrickson ,  George J. Vallone, III ,  Richard C. Muehling

    IPC: F16L37/02

    Abstract: Systems and methods for a tubing disconnect assembly are disclosed. In an embodiment the system includes a first bioprocessing unit, a second bioprocessing unit, a flexible tubing in fluid communication with the first and second bioprocessing unit, and a tubular collar having a middle portion sandwiched between a first and second swaged end portions, and secured around at least a portion of the tubing, the collar configured to be disconnected by a disconnecting device in the middle region extending between the first and second swaged end portions.

    CELL ANALYSIS USING CHEMFET SENSOR ARRAY-BASED SYSTEMS
    6.
    发明申请
    CELL ANALYSIS USING CHEMFET SENSOR ARRAY-BASED SYSTEMS 有权

    公开(公告)号：US20250116628A1

    公开(公告)日：2025-04-10

    申请号：US18922656

    申请日：2024-10-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wolfgang HINZ ,  John DONOHUE ,  Daniel BEACHMAN

    IPC: G01N33/543 ,  G01N27/30 ,  G03G5/02

    Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.

    Optical fiber pitch adjustment
    8.
    发明授权
    Optical fiber pitch adjustment 有权

    公开(公告)号：US12271043B2

    公开(公告)日：2025-04-08

    申请号：US17729480

    申请日：2022-04-26

    Applicant: Life Technologies Corporation

    Inventor： Derek Robinson ,  Wesley Smith ,  Keith Kearsley

    IPC: G02B6/42 ,  G01N15/1434

    Abstract: Provided are optical fiber alignment assemblies, comprising: a plurality of fiber channel members being arranged along a first vertical axis, each fiber channel member comprising a fiber channel configured to accommodate an optical fiber disposed therein, the plurality of fiber channels being parallel to one another, fiber channels adjacent to one another defining a spacing therebetween, the spacing being measured along the first vertical axis; a plurality of resilient members, a resilient member being disposed between adjacent fiber channel members; and an adjustment element, the adjustment element being configured to effect a force oriented along the first vertical axis, and the adjustment member being configured such that actuation of the adjustment member changes a compression of the plurality of resilient members so as to effect an essentially linear variation in the spacing between adjacent fiber channels. Also provided are related methods.

    MEMBRANE-PENETRATING PEPTIDES TO ENHANCED TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME
    9.
    发明申请
    MEMBRANE-PENETRATING PEPTIDES TO ENHANCED TRANSFECTION AND COMPOSITIONS AND METHODS FOR USING SAME 有权

    公开(公告)号：US20250109412A1

    公开(公告)日：2025-04-03

    申请号：US18911619

    申请日：2024-10-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Xavier De Mollerat Du Jeu

    IPC: C12N15/88 ,  A61K47/54 ,  A61K47/64 ,  C07K14/00

    Abstract: The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.

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