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公开(公告)号:US20170253921A1
公开(公告)日:2017-09-07
申请号:US15518016
申请日:2015-10-13
Inventor: Chunmei LIU , Shoulian DONG , Junko STEVENS
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6853 , C12Q1/686 , C12Q1/6883 , C12Q2537/16 , C12Q2537/161 , C12Q2545/107 , C12Q2545/113 , C12Q2600/16
Abstract: The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine CNV in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.
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公开(公告)号:US20250154531A1
公开(公告)日:2025-05-15
申请号:US18924629
申请日:2024-10-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Chi-Hsuan CHANG , Xiquan LIANG
IPC: C12N15/87 , C12N5/078 , C12N5/0783 , C12N9/22 , C12N15/11
Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.
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公开(公告)号:US12296308B2
公开(公告)日:2025-05-13
申请号:US17805301
申请日:2022-06-03
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Michael E. Goodwin , Wade A. Cornia , Jacob D. Lee
IPC: B01F27/88 , B01F27/113 , B01F27/2121 , B01F27/213 , B01F27/805 , B01F27/85 , B01F33/501 , B01F33/502 , B01F35/513 , B01F101/22 , C12M1/00 , C12M1/06
Abstract: A tube management system includes an elongated rack extending between a first end and an opposing second end; an elongated first shaft having a first end and an opposing second end, the first shaft being secured to the rack; a securing structure secured to the first shaft; a collapsible bag bounding a chamber; and a tubular member projecting from the collapsible bag, wherein the securing structure at least partially encircles the tubular member.
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公开(公告)号:US20250146605A1
公开(公告)日:2025-05-08
申请号:US18933336
申请日:2024-10-31
Applicant: LIFE TECHNOLOGIES CORPORATION
IPC: F16L37/02
Abstract: Systems and methods for a tubing disconnect assembly are disclosed. In an embodiment the system includes a first bioprocessing unit, a second bioprocessing unit, a flexible tubing in fluid communication with the first and second bioprocessing unit, and a tubular collar having a middle portion sandwiched between a first and second swaged end portions, and secured around at least a portion of the tubing, the collar configured to be disconnected by a disconnecting device in the middle region extending between the first and second swaged end portions.
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公开(公告)号:USD1073973S1
公开(公告)日:2025-05-06
申请号:US29841776
申请日:2022-06-08
Designer: Beng Heng Lim , Siu Wee Hon , Steven Huei Yeo , Joshua Mead , Roy Thompson , Xin Mathers , Victor Shapiro
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公开(公告)号:US20250116628A1
公开(公告)日:2025-04-10
申请号:US18922656
申请日:2024-10-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Wolfgang HINZ , John DONOHUE , Daniel BEACHMAN
IPC: G01N33/543 , G01N27/30 , G03G5/02
Abstract: Various cell analysis systems of the present teachings can measure the electrical and metabolic activity of single, living cells with subcellular addressability and simultaneous data acquisition for between about 10 cells to about 500,000 cells in a single analysis. Various sensor array devices of the present teachings can have sensor arrays with between 20 million to 660 million ChemFET sensors built into a massively paralleled array and can provide for simultaneous measurement of cells with data acquisition rates in the kilohertz (kHz) range. As various ChemFET sensor arrays of the present teachings can detect chemical analytes as well detect changes in cell membrane potential, various cell analysis systems of the present teachings also provide for the controlled chemical and electrical interrogation of cells.
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公开(公告)号:US12272538B2
公开(公告)日:2025-04-08
申请号:US17755179
申请日:2020-11-10
Applicant: Thermo Fisher Scientific (Bremen) GmbH , Life Technologies Corporation , FEI Deutschland GmbH
Inventor: Alexander Makarov , Michael Ward , Rainer Daum
Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device (110) includes an optical assembly (112), a sample stage (114), and a receiver (116). The optical assembly (112) is disposed in an inverted position below the sample stage (114) and the receiver (116) is positioned above the sample stage (112). The optical assembly enables imaging of a sample disposed on the sample stage (114). The optical assembly (112) also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume (20) toward the receiver (116).
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公开(公告)号:US12271043B2
公开(公告)日:2025-04-08
申请号:US17729480
申请日:2022-04-26
Applicant: Life Technologies Corporation
Inventor: Derek Robinson , Wesley Smith , Keith Kearsley
IPC: G02B6/42 , G01N15/1434
Abstract: Provided are optical fiber alignment assemblies, comprising: a plurality of fiber channel members being arranged along a first vertical axis, each fiber channel member comprising a fiber channel configured to accommodate an optical fiber disposed therein, the plurality of fiber channels being parallel to one another, fiber channels adjacent to one another defining a spacing therebetween, the spacing being measured along the first vertical axis; a plurality of resilient members, a resilient member being disposed between adjacent fiber channel members; and an adjustment element, the adjustment element being configured to effect a force oriented along the first vertical axis, and the adjustment member being configured such that actuation of the adjustment member changes a compression of the plurality of resilient members so as to effect an essentially linear variation in the spacing between adjacent fiber channels. Also provided are related methods.
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公开(公告)号:US20250109412A1
公开(公告)日:2025-04-03
申请号:US18911619
申请日:2024-10-10
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Xavier De Mollerat Du Jeu
Abstract: The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. Suitable cargo includes nucleic acid molecules (including DNA, RNA or PNA), polypeptides, or other biologically active molecules. The present invention is further directed to transfection complexes containing the non-naturally occurring peptides of the present invention in non-covalent association with at least one cationic lipid and a cargo to be delivered to the interior of a cell. The invention further relates to methods for the preparation and use of the non-naturally occurring peptides for the formation of transfection complexes and the delivery of a cargo to the interior of a cell in culture, an animal or a human. The invention also relates to compositions and kits useful for transfecting cells.
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公开(公告)号:US12263475B2
公开(公告)日:2025-04-01
申请号:US17970202
申请日:2022-10-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor: Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
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