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公开(公告)号：US20090304669A1
公开(公告)日：2009-12-10
申请号：US12124390
申请日：2008-05-21
Applicant: Peter Matthiessen , Stefan Romeder-Finger , Peter Turecek , Hans-Peter Schwarz
Inventor： Peter Matthiessen , Stefan Romeder-Finger , Peter Turecek , Hans-Peter Schwarz
CPC classification number: C12N9/6454 , B01D15/363 , C12Y304/21075
Abstract: Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on Capto-MMC™ resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after Capto-MMC™ chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
Abstract translation: 重组截短的人弗林蛋白酶在CHO细胞中表达，并通过超滤和渗滤浓缩约50倍。 浓缩物通过Capto-MMC TM上的柱色谱纯化，得到30-50倍纯化因子，产率至少为60％。 在Capto-MMC TM色谱上获得的至少20％的纯制剂已经具有允许亲VWF的柱上成熟的纯化程度。 然后进行额外的精氨酸琼脂糖层析纯化。 用于纯化截短的人弗林蛋白酶的这种两柱方法产生几乎纯的弗林蛋白酶制剂，其具有约290,000U弗林蛋白/ mg蛋白质的比活性，约50％的产率。
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2.发明授权公开(公告)号：US06395880B1
公开(公告)日：2002-05-28
申请号：US09509052
申请日：2000-05-26
Applicant: Yendra Linnau , Ernst Hetzl , H. Peter Matthiessen , Silvia Neppl , Wolfgang Schönhofer , Hans-Peter Schwarz
Inventor： Yendra Linnau , Ernst Hetzl , H. Peter Matthiessen , Silvia Neppl , Wolfgang Schönhofer , Hans-Peter Schwarz
IPC: C12N974
CPC classification number: C07K14/8128 , A61K38/00
Abstract: A method of purifying antithrombin III (AT III) from a starting material containing an AT III/heparin complex or an AT III/heparinoid complex is disclosed. First, the method comprises adsorbing the AT III/heparin complex or the AT III/heparinoid complex on an anion exchanger material. Second, the method involves separating the AT III from the adsorbed AT III/heparin complex or an AT III/heparinoid complex by elution with a buffer having a pH ranging from 8.5 to 10.5 and a conductivity between 10 and 60 mS.
Abstract translation: 公开了从含有AT III /肝素复合物或AT III /类肝素复合物的原料中纯化抗凝血酶III（AT III）的方法。 首先，该方法包括在阴离子交换材料上吸附AT III /肝素复合物或AT III /类肝素复合物。 第二，该方法包括通过用pH为8.5至10.5和10至60mS的电导率的缓冲液洗脱来分离AT III与吸附的AT III /肝素复合物或AT III /类肝素复合物。
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3.发明授权Methods for the inactivation of microorganisms in biological fluids, flow through reactors and methods of controlling the light sum dose to effectively inactivate microorganisms in batch reactors 有权生物流体中微生物灭活的方法，流经反应器的方法和控制光总剂量以有效灭活间歇反应器中的微生物的方法公开(公告)号：US08377375B2
公开(公告)日：2013-02-19
申请号：US13100570
申请日：2011-05-04
Applicant: Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
Inventor： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
CPC classification number: A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
Abstract: The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
Abstract translation: 本发明涉及一种用于确定来自一种或多种光源的有效剂量的单色或多色光以灭活存在于生物流体中，优选非透明流体中的微生物的方法。 此外，提供了在流通反应器中的生物流体中的微生物灭活的方法。 此外，本发明有利地提供具有一个或多个恒温光源的流通反应器。 本发明还提供一种控制从一个或多个光源发射的单色或多色光的光总量的方法，以有效地灭活间歇反应器中存在于生物流体中的微生物。
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4.发明授权Methods for the inactivation of microorganisms in biological fluids, flow through reactors and methods of controlling the light sum dose to effectively inactivate microorganisms in batch reactors 有权生物流体中微生物灭活的方法，流经反应器的方法和控制光总剂量以有效灭活间歇反应器中的微生物的方法公开(公告)号：US07993580B2
公开(公告)日：2011-08-09
申请号：US10924579
申请日：2004-08-24
Applicant: Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
Inventor： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
CPC classification number: A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
Abstract: The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
Abstract translation: 本发明涉及一种用于确定来自一种或多种光源的有效剂量的单色或多色光以灭活存在于生物流体中，优选非透明流体中的微生物的方法。 此外，提供了在流通反应器中的生物流体中的微生物灭活的方法。 此外，本发明有利地提供具有一个或多个恒温光源的流通反应器。 本发明还提供了一种控制从一个或多个光源发射的单色或多色光的光总量的方法，以有效地灭菌间歇反应器中存在于生物流体中的微生物。
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公开(公告)号：US07807435B2
公开(公告)日：2010-10-05
申请号：US11202349
申请日：2005-08-11
Applicant: Peter Matthiessen , Gerald Brachtl , Peter Turecek , Hans-Peter Schwarz
Inventor： Peter Matthiessen , Gerald Brachtl , Peter Turecek , Hans-Peter Schwarz
IPC: C12N9/99
CPC classification number: C07K14/8125
Abstract: The present invention relates to a method for the purification of alpha-1 proteinase inhibitor (a1PI) from protein fractions. More specifically, the invention relates to an improved method for the purification of alpha-1 proteinase inhibitor (a1PI), wherein the yield of a1PI can be increased by thawing the starting material and incubating it for several hours before subjecting it to a washing step.
Abstract translation: 本发明涉及从蛋白质级分纯化α-1蛋白酶抑制剂（a1PI）的方法。 更具体地说，本发明涉及一种纯化α-1蛋白酶抑制剂（a1PI）的改进方法，其中可以通过解冻起始物质并将其孵育数小时，然后使其进行洗涤步骤，从而增加a1PI的产率。
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Patent Agency Ranking
公开(公告)号：US20070037270A1
公开(公告)日：2007-02-15
申请号：US11202349
申请日：2005-08-11
Applicant: Peter Matthiessen , Gerald Brachtl , Peter Turecek , Hans-Peter Schwarz
Inventor： Peter Matthiessen , Gerald Brachtl , Peter Turecek , Hans-Peter Schwarz
IPC: C12N9/99
CPC classification number: C07K14/8125
Abstract: The present invention relates to a method for the purification of alpha-1 proteinase inhibitor (a1PI) from protein fractions. More specifically, the invention relates to an improved method for the purification of alpha-1 proteinase inhibitor (a1PI), wherein the yield of a1PI can be increased by thawing the starting material and incubating it for several hours before subjecting it to a washing step.
Abstract translation: 本发明涉及从蛋白质级分纯化α-1蛋白酶抑制剂（a1PI）的方法。 更具体地说，本发明涉及一种纯化α-1蛋白酶抑制剂（a1PI）的改进方法，其中可以通过解冻起始物质并将其孵育数小时，然后使其进行洗涤步骤，从而增加a1PI的产率。
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公开(公告)号：US09388452B2
公开(公告)日：2016-07-12
申请号：US13638919
申请日：2011-04-08
Applicant: Peter Matthiessen
Inventor： Peter Matthiessen
IPC: G01N33/48 , G01N33/50 , C12Q1/56 , G06F19/16 , G06F17/10 , G06F17/11 , G06F19/12 , C07K14/755 , C07K14/81 , G01N33/68
CPC classification number: C12Q1/56 , C07K14/755 , C07K14/8128 , G01N33/6803 , G01N2333/755 , G01N2333/8128 , G06F17/10 , G06F17/11 , G06F19/12 , G06F19/16
Abstract: The present invention relates to a method for determining the highest temperature that is suitable for performing accelerated protein stability studies, as well as to a method for modeling real-time protein stability from accelerated stability data generated at said temperature.
Abstract translation: 本发明涉及一种用于确定适合进行加速蛋白质稳定性研究的最高温度的方法，以及用于通过在所述温度下产生的加速稳定性数据建模实时蛋白稳定性的方法。
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公开(公告)号：US08343748B2
公开(公告)日：2013-01-01
申请号：US12124390
申请日：2008-05-21
Applicant: Peter Matthiessen , Stefan Romeder-Finger , Peter Turecek , Hans-Peter Schwarz
Inventor： Peter Matthiessen , Stefan Romeder-Finger , Peter Turecek , Hans-Peter Schwarz
CPC classification number: C12N9/6454 , B01D15/363 , C12Y304/21075
Abstract: Recombinant truncated human furin was expressed in CHO cells and concentrated approximately 50-fold by ultrafiltration and diafiltration. The concentrate was purified by column chromatography on CAPTO MMC™ (mixed cation exchange/hydrophobic interaction gel) resulting in a 30-50 fold purification factor and a yield of at least 60%. The at least 20% pure preparation obtained after CAPTO MMC™ (mixed cation exchange/hydrophobic interaction gel) chromatography had already a purification degree allowing on-column maturation of pro-VWF. Then an additional Arginine Sepharose chromatography purification was carried out. This two column process for purification of truncated human furin resulted in an almost pure furin preparation with a specific activity of approximately 290,000 U furin/mg protein and a yield of about 50%.
Abstract translation: 重组截短的人弗林蛋白酶在CHO细胞中表达，并通过超滤和渗滤浓缩约50倍。 浓缩物通过CAPTO MMC TM（混合阳离子交换/疏水相互作用凝胶）上的柱色谱纯化，得到30-50倍纯化因子，产率至少为60％。 在CAPTO MMC TM（混合阳离子交换/疏水相互作用凝胶）色谱之后获得的至少20％的纯制剂已经具有允许亲VWF的柱上成熟的纯化度。 然后进行额外的精氨酸琼脂糖层析纯化。 用于纯化截短的人弗林蛋白酶的这种两柱方法产生几乎纯的弗林蛋白酶制剂，其具有约290,000U弗林蛋白/ mg蛋白质的比活性，约50％的产率。
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9.发明申请METHODS FOR THE INACTIVATION OF MICROORGANISMS IN BIOLOGICAL FLUIDS, FLOW THROUGH REACTORS AND METHODS OF CONTROLLING THE LIGHT SUM DOSE TO EFFECTIVELY INACTIVATE MICROORGANISMS IN BATCH REACTORS 有权生物流体中微生物灭活的方法，通过反应器流动的方法和控制轻剂量以有效地使分批反应器中的微生物失活的方法公开(公告)号：US20110206554A1
公开(公告)日：2011-08-25
申请号：US13100570
申请日：2011-05-04
Applicant: Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
Inventor： Heinz Anderle , Peter Matthiessen , Hans-Peter Schwarz , Peter Turecek , Thomas Kreil , Daniel R. Boggs
CPC classification number: A61L2/10 , A23L3/26 , A23L3/28 , A61L2/0011 , A61L2/28 , G01N21/33 , H05B3/0052
Abstract: The present invention relates to a method for determining an effective dose of monochromatic or polychromatic light from one or more light sources to inactivate microorganisms present in a biological fluid, preferably a non-transparent fluid. Moreover, there is provided a method for the inactivation of microorganism in a biological fluid in a flow-through-reactor. Moreover, the invention advantageously provides a flow-through-reactor with one or more thermostated light sources. The invention further provides a method of controlling the light sum dose of monochromatic or polychromatic light emitted from one or more light sources to effectively inactivate microorganisms present in a biological fluid in a batch reactor.
Abstract translation: 本发明涉及一种用于确定来自一种或多种光源的有效剂量的单色或多色光以灭活存在于生物流体中，优选非透明流体中的微生物的方法。 此外，提供了在流通反应器中的生物流体中的微生物灭活的方法。 此外，本发明有利地提供具有一个或多个恒温光源的流通反应器。 本发明还提供一种控制从一个或多个光源发射的单色或多色光的光总量的方法，以有效地灭活间歇反应器中存在于生物流体中的微生物。
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公开(公告)号：US20090192076A1
公开(公告)日：2009-07-30
申请号：US12342646
申请日：2008-12-23
Applicant: PETER MATTHIESSEN , PETER TURECEK , HANS-PETER SCHWARZ , KURT SCHNECKER
Inventor： PETER MATTHIESSEN , PETER TURECEK , HANS-PETER SCHWARZ , KURT SCHNECKER
IPC: A61K38/16
CPC classification number: A61K47/26 , A61K9/0019 , A61K9/08 , A61K38/1709 , A61K38/36 , A61K47/02 , A61K47/10 , A61K47/12 , A61K47/18 , A61K47/20 , A61K47/22
Abstract: The present invention provides long-term stable pharmaceutical formulations of recombinant von-Willebrand Factor (rVWF) and methods for making and administering said formulations.
Abstract translation: 本发明提供重组血管性血友病因子（rVWF）的长期稳定的药物制剂以及制备和施用所述制剂的方法。
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