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公开(公告)号：US20240425840A1
公开(公告)日：2024-12-26
申请号：US18680688
申请日：2024-05-31
Applicant: Spogen Biotech Inc.
Inventor： Brian M. Thompson , Jorg Augustin , Ashley Siegel
IPC: C12N9/78 , A01N63/22 , A23K10/18 , A23K20/189 , A61K9/00 , A61K35/742 , A61K38/00 , C07K14/32 , C12N9/24 , C12N9/42
Abstract: Fusion proteins containing a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member are provided. The fusion proteins further comprise an enzyme having ACC deaminase activity, a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a glucanase, a phytase, an acid phosphatase, a pectinase, a mannanase, and/or an expansin protein. Also provided are recombinant Bacillus cereus family members that express the fusion proteins, exosporium fragments derived from the recombinant Bacillus cereus family members, and formulations containing the recombinant Bacillus cereus family members or exosporium fragments. Plant seeds treated with the recombinant Bacillus cereus family members, exosporium fragments, or formulations are also provided. The invention further relates to methods for stimulating plant growth and/or promoting plant health using the recombinant Bacillus cereus family members, exosporium fragments, or formulations.
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2.发明申请公开(公告)号：US20230134066A1
公开(公告)日：2023-05-04
申请号：US17932994
申请日：2022-09-16
Applicant: Spogen Biotech Inc.
Inventor： Brian M. Thompson , Jorg Augustin
IPC: C05F11/08 , C05B15/00 , C12N9/78 , C05G3/60 , A01N63/50 , C05F17/20 , C05C9/00 , C07K14/415 , C12N1/20 , C12N15/82
Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.
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公开(公告)号：US20220087268A1
公开(公告)日：2022-03-24
申请号：US17540823
申请日：2021-12-02
Applicant: Spogen Biotech Inc.
Inventor： Brian Thompson , Michelle Leslie
IPC: A01N63/50 , C07K14/195 , C07K14/32 , C12N1/20 , C12P21/02 , A01N63/10 , A01N63/22 , C07K14/27 , A01N63/25 , C07K14/21 , A01N37/46 , C07K14/245
Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.
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公开(公告)号：US11134681B2
公开(公告)日：2021-10-05
申请号：US16785870
申请日：2020-02-10
Applicant: Spogen Biotech Inc.
Inventor： Brian Thompson , Katie Thompson
IPC: C12N9/20 , C07K14/32 , A01N37/46 , A01N63/50 , A01N63/22 , A01N63/10 , A01N63/00 , C12N9/02 , C12N9/16 , C12N9/24 , A01N37/48 , C12N9/42 , C12N9/48
Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.
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5.发明申请公开(公告)号：US20200107552A1
公开(公告)日：2020-04-09
申请号：US16508524
申请日：2019-07-11
Applicant: Spogen Biotech Inc.
Inventor： Brian Thompson , Katie Thompson
IPC: A01N63/00 , A01N37/46 , C07K14/32 , C12N9/02 , C12N9/16 , C12N9/24 , A01N37/48 , C12N9/20 , C12N9/42 , C12N9/48
Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.
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Patent Agency Ranking
公开(公告)号：US10428347B2
公开(公告)日：2019-10-01
申请号：US16043477
申请日：2018-07-24
Applicant: Spogen Biotech Inc. , Nufarm Limited
Inventor： Brian Thompson
Abstract: Herbicide-detoxifying enzymes, compositions containing one or more of the enzymes, and plant seeds treated with the enzymes are provided. The enzymes can be used in methods for detoxifying auxin herbicides or degrading auxin plant regulators, including in methods for decontaminating surface of an apparatus used in agriculture or pesticide manufacturing, methods for decontaminating water, soil, soilless media, or sludge, and methods for protecting a plant from an auxin herbicide, improving a plant's tolerance to an auxin herbicide, or removing an auxin herbicide from the surface of a plant.
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公开(公告)号：US20170295798A1
公开(公告)日：2017-10-19
申请号：US15511854
申请日：2015-09-17
Applicant: Bayer CropScience LP , Spogen Biotech Inc.
Inventor： Damian CURTIS , Brian THOMPSON
CPC classification number: A01N63/00 , A01N43/54 , A01N43/56 , A01N47/24 , A01N47/26 , C12Y301/00 , C12Y302/01004 , C12Y302/01132 , A01N37/46 , A01N43/32 , A01N43/36 , A01N43/653 , A01N55/00
Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Baciillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Baciillus cells; and b) at least one particular fungicide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.
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8.发明申请公开(公告)号：US20170283472A1
公开(公告)日：2017-10-05
申请号：US15511839
申请日：2015-09-17
Applicant: Bayer CropScience LP , Spogen Biotech Inc.
Inventor： Damian CURTIS , Brian THOMPSON
IPC: C07K14/325 , C12N15/82 , C12N15/866 , C12N15/63
CPC classification number: C07K14/325 , A01N25/00 , A01N63/00 , A61K38/00 , C12N15/00 , C12N15/63 , C12N15/82 , C12N15/866 , A01N63/04
Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and/or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and/or at least one metabolite produced by the respective strain, and/or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and/or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.
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公开(公告)号：US20160316761A1
公开(公告)日：2016-11-03
申请号：US15211044
申请日：2016-07-15
Applicant: Spogen Biotech Inc.
Inventor： Brian Thompson , Katie Thompson , Brittany Angle
CPC classification number: A01N63/00 , A01H3/00 , A01N63/02 , C12R1/01 , C12R1/05 , C12R1/07 , C12R1/075 , C12R1/085 , C12R1/125 , A01N25/00
Abstract: The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.
Abstract translation: 本发明一般涉及植物生长促进细菌新菌株的细菌学纯细菌培养物和包含其的接种物。 本发明还涉及用接种物包被的植物种子，包含接种物的试剂盒和通过将生物学纯细菌培养物或接种物施用于植物，植物种子或植物生长培养基来刺激植物生长的方法。
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10.发明申请FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS 有权融合蛋白和刺激植物生长的方法，保护植物和植物上的苗木运动公开(公告)号：US20160031948A1
公开(公告)日：2016-02-04
申请号：US14775892
申请日：2014-03-17
Applicant: SPOGEN BIOTECH INC.
Inventor： Brian Thompson , Katie Thompson
CPC classification number: C07K14/32 , A01N37/46 , A01N37/48 , A01N63/00 , A01N63/02 , C07K2319/035 , C12N9/0095 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2437 , C12N9/48 , C12Y118/06001 , C12Y301/01003 , C12Y301/03008 , C12Y302/01004 , C12Y304/00
Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.
Abstract translation: 本发明一般涉及含有将融合蛋白靶向蜡状芽孢杆菌家族成员的外植体的靶向序列的融合蛋白。 本发明还涉及表达这种融合蛋白的重组蜡状芽孢杆菌家族成员和含有表达融合蛋白的重组蜡状芽孢杆菌家族成员的制剂。 还描述了通过将重组蜡状芽孢杆菌家族成员或制剂施用于植物或植物生长培养基来刺激植物生长，保护植物免受病原体和增强植物胁迫抗性的方法。 本发明还涉及将表达融合蛋白的重组蜡状芽孢杆菌家族成员的孢子固定在植物上的方法。
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