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公开(公告)号：US12087568B2
公开(公告)日：2024-09-10
申请号：US18456678
申请日：2023-08-28
Applicant: Standard BioTools Canada Inc.
Inventor： Alexander Loboda , Raymond Jong , Michael Sullivan , Serguei Vorobiev , Robert Rotenberg , Emil D. Stratulativ , Maxim Voronov , Mark Armstrong
CPC classification number: H01J49/105 , H01J49/0027 , H01J49/0463 , H05H1/2406 , H05H1/30
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：US20240371622A1
公开(公告)日：2024-11-07
申请号：US18610570
申请日：2024-03-20
Applicant: Standard BioTools Canada Inc. , University of Ottawa
Inventor： Paul Corkum , Alexander V. Loboda
IPC: H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/14 , H01J49/16 , H01J49/40
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC®) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US20250044571A1
公开(公告)日：2025-02-06
申请号：US18887633
申请日：2024-09-17
Applicant: Standard BioTools Canada Inc.
Inventor： Daaf Sandkuijl , Alexander V. Loboda , Adam Carew , Khashayar Askarpour
Abstract: We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.
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公开(公告)号：US20230215714A1
公开(公告)日：2023-07-06
申请号：US17785887
申请日：2020-12-21
Applicant: Standard BioTools Canada Inc.
Inventor： Adam Carew , Daaf Sandkuijl , Alexander Loboda , Matthew Weel , Dmitry Bandura
CPC classification number: H01J49/0004 , H01J49/40 , H01J49/0463
Abstract: Described herein are systems and methods for imaging mass spectrometry, including imaging mass cytometry. Aspects of the subject application include apparatus and methods for imaging mass spectrometry (IMS) that improve speed of sample acquisition, signal sensitivity, and/or signal stability. Systems and methods described herein may minimize the transfer time and/or may minimize the spread of plumes of sample material ablated from a sample to be transferred to the components of the imaging mass spectrometer or mass cytometer that ionize and analyze the sample material.
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公开(公告)号：US20250093252A1
公开(公告)日：2025-03-20
申请号：US18726639
申请日：2023-01-09
Applicant: Standard BioTools Canada Inc.
Inventor： Alexander Loboda , Adam Carew
IPC: G01N15/1404 , G01N15/10 , H01J49/00 , H01J49/04 , H01J49/10
Abstract: A coupling tube made of a stretchable elastomeric material. The “stretchy” coupling tubing allows for improved reliability/repeatability of coupling performance. For example, the stretch provided can take up tolerances between positions of imager and reader instruments. In one example, the stretch can take up tolerances between positions of the junction between the rigid portion of tubing inside the IMC unit and the injector to an ionization source. The coupling tube may also be provided with a reduced inner diameter (ID) as compared to standard coupling components. A taper in the inner diameter (ID) can occur before the flow enters the stretchable coupling tube. Barbed ends may also be provided for securing the coupling tube into place.
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Patent Agency Ranking
公开(公告)号：US20240345072A1
公开(公告)日：2024-10-17
申请号：US18409399
申请日：2024-01-10
Applicant: Standard BioTools Canada Inc.
Inventor： Olga Ornatsky , Qing Chang , Bedilu Allo , Xudong Lou , Alexander Bouzekri
IPC: G01N33/532 , C07K1/13 , G01N33/58
CPC classification number: G01N33/532 , C07K1/13 , G01N33/583
Abstract: The present disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.
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公开(公告)号：US20230236105A1
公开(公告)日：2023-07-27
申请号：US17786163
申请日：2020-12-18
Applicant: Standard BioTools Canada Inc.
Inventor： Bedilu ALLO , Xudong LOU , Peng LIU , Daniel MAJONIS , Anastasia MAVROPOULOS , Ladan GHEIRATMAND
IPC: G01N15/14 , G01N33/483
CPC classification number: G01N15/1429 , G01N33/483 , G01N2015/1006
Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.
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公开(公告)号：US20250138010A1
公开(公告)日：2025-05-01
申请号：US18692892
申请日：2022-09-22
Applicant: Standard BioTools Canada Inc.
Inventor： Colin Thom
IPC: G01N33/569 , G01N33/53 , G01N33/68
Abstract: Aspects of the subject application include applying a spatial barcode to a cellular sample and then performing suspension mass cytometry on the cellular sample. For example, a sample barcode comprising a known distribution of sample barcode isotopes may be applied to the sample such that cells in different locations receive a unique combination or ratio of barcode isotopes, after which cells are suspended (e.g., dissociated from tissue) and processed by suspension mass cytometry. While barcode isotopes are described in a number of embodiments herein, non-enriched elements may be used instead of, or in addition to, enriched isotopes. Mass cytometry methods and reagents are discussed below, followed by a further description of spatial barcoding and kits thereof.
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公开(公告)号：US12278098B2
公开(公告)日：2025-04-15
申请号：US18610570
申请日：2024-03-20
Applicant: Standard BioTools Canada Inc. , University of Ottawa
Inventor： Paul Corkum , Alexander V. Loboda
IPC: H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/14 , H01J49/16 , H01J49/40
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC™) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US20240369567A1
公开(公告)日：2024-11-07
申请号：US18644592
申请日：2024-04-24
Applicant: Standard BioTools Canada Inc.
Inventor： Alexander V. Loboda , Olga Ornatsky , Vladimir Baranov , Nikita Zabinyakov , Anastasia Mavropoulos , Daniel Majonis , Bedilu Allo , Taunia Closson
Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. Embodiments include a method for quantifying one or more analytes within a sample. The method may include providing the sample. One or more analytes may be immobilised to a mass cytometry sample carrier. The sample may have been labelled with one or more mass-tagged SBPs including one or more labelling atoms. The method may include performing mass cytometry on the sample to determine a level of the one or more labelling atoms, where the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes to quantify the analytes.
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