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    FET-type biosensor with surface modification
    1.
    发明授权
    FET-type biosensor with surface modification 有权
    具有表面改性的FET型生物传感器

    公开(公告)号：US08735077B2

    公开(公告)日：2014-05-27

    申请号：US11336110

    申请日：2006-01-20

    Applicant: Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

    Inventor： Jeo-young Shim ,  Su-hyeon Kim ,  Kyu-tae Yoo ,  Sung-ouk Jung ,  Joon-shik Park

    IPC: G01N33/53

    CPC classification number: B82Y30/00 ,  B82Y15/00 ,  G01N27/4145

    Abstract: Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.

    Abstract translation: 提供了包括源电极，栅极和漏电极的场效应晶体管（FET）型生物传感器。 可以结合核酸一侧的配体加入到门的表面。 在传统的FET型生物传感器中，由于目标核酸直接固定在常规FET的栅极表面，难以检测德拜长度内的信号。 然而，在本发明中，可以克服这个问题，并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。 配体可以吸附在栅极的表面上。 在这种情况下，核酸被平行于栅极的表面吸附，而不垂直于栅极的表面，从而产生有效的耗尽区域。 此外，杂交效率可以提高，因为杂化样品可以以高离子强度注入FET传感器。

    Fluorescence detector for detecting microfluid
    6.
    发明授权
    Fluorescence detector for detecting microfluid 有权
    用于检测微流体的荧光检测器

    公开(公告)号：US07327459B2

    公开(公告)日：2008-02-05

    申请号：US10933084

    申请日：2004-09-02

    Applicant: Su-hyeon Kim ,  Jin-tae Kim

    Inventor： Su-hyeon Kim ,  Jin-tae Kim

    IPC: G01N21/25

    CPC classification number: G01N21/645 ,  G01N2021/6482

    Abstract: An ultra small fluorescence detector capable of detecting in real time reaction undergoing in a micro chamber having a predetermined volume and disposed on a microfluid chip is provided. The fluorescence detector for detecting in real time PCR amplification undergoing in the microfluid chip having a micro chamber with a predetermined volume includes a light source generating an excitation beam, a first optical system capable of irradiating the excitation beam having a predetermined spot size to the micro chamber, a first detector, and a second optical system reflecting a fluorescent beam derived from the excitation beam having the predetermined spot size in the micro chamber to the first detector. Accordingly, the fluorescence detector is designed such that light emitted by a light source is focused between a first mirror and an objective lens. Therefore, the spot size of an excitation beam transmitted by the objective lens is largely formed so that the excitation beam can be irradiated on the whole micro chamber of the microfluid chip, thereby detecting a fluorescent beam on a broader area.

    Abstract translation: 提供了能够实时检测具有预定体积的微室中并设置在微流体芯片上的超小荧光检测器。 用于实时检测在具有预定体积的微室的微流体芯片中进行PCR扩增的荧光检测器包括产生激发光束的光源，能够将具有预定光斑尺寸的激发光束照射到微细的第一光学系统 第一检测器和第二光学系统，其将来自具有在微室中的预定光斑尺寸的激发光束的荧光束反射到第一检测器。 因此，荧光检测器被设计成使得由光源发射的光聚焦在第一反射镜和物镜之间。 因此，由物镜透射的激发光束的光斑尺寸大大地形成为使得激发光束可以照射在微流体芯片的整个微室上，从而在更广泛的区域上检测荧光束。

    DEVICE AND METHOD FOR RAPIDLY LYSING CELLS OR VIRUSES
    7.
    发明申请
    DEVICE AND METHOD FOR RAPIDLY LYSING CELLS OR VIRUSES 失效
    用于快速诱导细胞或病毒的装置和方法

    公开(公告)号：US20080014122A1

    公开(公告)日：2008-01-17

    申请号：US11560890

    申请日：2006-11-17

    Applicant: Su-hyeon KIM ,  Jeong-gun LEE

    Inventor： Su-hyeon KIM ,  Jeong-gun LEE

    IPC: B01L3/00

    CPC classification number: C12M47/06 ,  B01L3/5027 ,  C12N1/066 ,  C12N13/00 ,  G01N1/44 ,  Y10T137/2496 ,  Y10T436/25

    Abstract: A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provide. Further, a method of lysing cells or viruses using the cell lysis device is provide. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.

    Abstract translation: 一种用于裂解细胞或病毒的细胞裂解装置，包括具有样品入口的细胞裂解管; 连接到细胞裂解管的泵，用于将样品转移到管中; 用于可逆地密封管的特定区域的密封单元; 并提供用于产生激光的激光源。 此外，提供使用细胞裂解装置裂解细胞或病毒的方法。 该方法包括通过样品入口将含有细胞或病毒的样品和任选的磁珠引入细胞裂解管; 通过泵将样品转移到细胞裂解管中的特定区域; 用密封单元临时密封放置样品的细胞溶解管的区域; 用激光照射样品; 从细胞裂解管中取出密封单元; 并通过泵从细胞裂解管中排出样品。

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