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1.发明申请公开(公告)号：US20100136621A1
公开(公告)日：2010-06-03
申请号：US12692665
申请日：2010-01-25
Applicant: Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Lisa Diane Reiss , Pierre E. Rouviere
Inventor： Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Lisa Diane Reiss , Pierre E. Rouviere
CPC classification number: C12P21/02 , C07K2319/50 , C12N9/90
Abstract: Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
Abstract translation: 公开了用于产生不溶性融合肽的肽标签，这里称为包涵体标签。 融合肽包含可操作地连接到目的肽的至少一种包涵体标签。 融合肽在宿主细胞中的表达产生不溶性并且包含在细胞和/或细胞裂解物中的包涵体内的产物。 然后可以纯化包涵体，并且在从包涵体标签中切割后可以分离感兴趣的蛋白质。
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公开(公告)号：US08617843B2
公开(公告)日：2013-12-31
申请号：US13080917
申请日：2011-04-06
Applicant: Albert W. Alsop , Qiong Cheng , Linda Jane Solomon , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
Inventor： Albert W. Alsop , Qiong Cheng , Linda Jane Solomon , Stephen R. Fahnestock , Tanja Maria Gruber , Pierre E. Rouviere
CPC classification number: C12P21/02 , C07K2319/00 , C12P21/06
Abstract: The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.
Abstract translation: 本发明涉及以包含溶解性标签的融合蛋白形式的目标肽的重组表达。 融合蛋白包含由可切割肽序列分开的至少两个部分，其中一部分缺少半胱氨酸残基，第二部分包含有效数目的可交联半胱氨酸残基。 细胞裂解和分离融合蛋白后，融合蛋白随后被切割成第一和第二部分的混合物。 氧化交联用于选择性沉淀两部分之一，以促进目的肽的简单和有效分离。
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公开(公告)号：US08354381B2
公开(公告)日：2013-01-15
申请号：US12732248
申请日：2010-03-26
Applicant: Stephen R. Fahnestock , Kari A. Fosser , Hong Wang , Pierre E. Rouviere , Tanja Maria Gruber , Douglas Robert Anton
Inventor： Stephen R. Fahnestock , Kari A. Fosser , Hong Wang , Pierre E. Rouviere , Tanja Maria Gruber , Douglas Robert Anton
CPC classification number: A61K8/25 , A61K8/0241 , A61K8/29 , A61K8/64 , A61K8/731 , A61K2800/412 , A61K2800/43 , A61K2800/614 , A61Q11/00 , C07K7/08 , C07K14/001
Abstract: Oral care compositions, oral care systems, oral surface-binding peptides, and a method for applying particles to an oral surface are provided. The oral care system comprises at least one peptidic component comprising a first binding element having affinity for an oral surface and a second binding element having affinity for a ligand property of a particle.
Abstract translation: 提供了口腔护理组合物，口腔护理系统，口腔表面结合肽以及将颗粒施用于口腔表面的方法。 口腔护理系统包含至少一种肽组分，其包含对口腔表面具有亲和力的第一结合元件和对颗粒的配体性质具有亲和力的第二结合元件。
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公开(公告)号：US20120122153A1
公开(公告)日：2012-05-17
申请号：US13226610
申请日：2011-09-07
Applicant: LAURA A. BEDZYK , Stephen R. Fahnestock , Tanja Maria Gruber , Daniel P. Okeefe , Pierre E. Rouviere
Inventor： LAURA A. BEDZYK , Stephen R. Fahnestock , Tanja Maria Gruber , Daniel P. Okeefe , Pierre E. Rouviere
CPC classification number: C07K5/1021 , C07K1/12 , C07K7/06 , C07K14/435 , C07K2319/00 , C07K2319/01 , C07K2319/50 , C12N15/62 , C12P21/06
Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.
Abstract translation: 公开了包含天冬氨酸和脯氨酸残基的可酸切割的肽接头。 酸可切割肽接头提供对式PEP1-L-PEP2的融合肽对感兴趣的肽的酸水解释放的改变的敏感性。 在各种实施方案中描述了本发明的接头L，其各自提供比单一天冬氨酸 - 脯氨酸对显着更快速地释放目的肽。 在另一方面，还提供了提高酸可切割键与酸水解的稳定性的方法。
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公开(公告)号：US20100247590A1
公开(公告)日：2010-09-30
申请号：US12748685
申请日：2010-03-29
Applicant: Douglas Robert Anton , Susan Daly , Robert J. Bianchini , Hong Wang , Pierre E. Rouviere , Scott D. Cunningham , Stephen R. Fahnestock , Tanja Maria Gruber
Inventor： Douglas Robert Anton , Susan Daly , Robert J. Bianchini , Hong Wang , Pierre E. Rouviere , Scott D. Cunningham , Stephen R. Fahnestock , Tanja Maria Gruber
IPC: A61K8/02 , A61K9/14 , A61Q17/04 , A61K8/64 , A61P17/10 , A61P31/10 , A61P31/04 , A61Q9/00 , A61Q5/00
CPC classification number: A61K8/64 , A61K8/19 , A61K2800/88 , A61K2800/884 , A61K2800/94 , A61Q5/10
Abstract: The present invention relates to compositions and systems comprising peptide-based reagents for delivery of cosmetic benefit agents to human hair, human skin, or human nail.
Abstract translation: 本发明涉及包含用于向人类头发，人类皮肤或人类指甲递送化妆品有益剂的基于肽的试剂的组合物和系统。
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Patent Agency Ranking
6.发明授权Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins 失效在用于产生肽或蛋白质的原核细胞的荧光激活细胞分选分析中使用四羧酸标签公开(公告)号：US07794963B2
公开(公告)日：2010-09-14
申请号：US12263608
申请日：2008-11-03
Applicant: Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
Inventor： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
IPC: G01N33/569 , C07K16/00
CPC classification number: C12N15/62 , C07K2319/20 , C07K2319/60
Abstract: A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.
Abstract translation: 体内标记和鉴定重组产生的肽或蛋白质在未经过预处理的原核宿主细胞内的过程。 表达含有至少一个四羧酸标签的融合肽的重组原核细胞在体内用双标记标记试剂进行标记。 使用荧光活化细胞分选器来鉴定和选择荧光细胞亚群，其中细胞中融合肽的量与所检测的荧光量成比例。
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7.发明授权公开(公告)号：US07678883B2
公开(公告)日：2010-03-16
申请号：US11782836
申请日：2007-07-25
Applicant: Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Lisa Diane Reiss , Pierre E. Rouviere
Inventor： Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Lisa Diane Reiss , Pierre E. Rouviere
CPC classification number: C12P21/02 , C07K2319/50 , C12N9/90
Abstract: Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
Abstract translation: 公开了用于产生不溶性融合肽的肽标签，这里称为包涵体标签。 融合肽包含可操作地连接到目的肽的至少一种包涵体标签。 融合肽在宿主细胞中的表达产生不溶性并且包含在细胞和/或细胞裂解物中的包涵体内的产物。 然后可以纯化包涵体，并且在从包涵体标签中切割后可以分离感兴趣的蛋白质。
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公开(公告)号：US08652455B2
公开(公告)日：2014-02-18
申请号：US13330171
申请日：2011-12-19
Applicant: Robert DiCosimo , Scott D. Cunningham , Stephen R. Fahnestock , Tanja Maria Gruber , Mark S. Payne , Pierre E. Rouviere , Linda Jane Solomon , Hong Wang
Inventor： Robert DiCosimo , Scott D. Cunningham , Stephen R. Fahnestock , Tanja Maria Gruber , Mark S. Payne , Pierre E. Rouviere , Linda Jane Solomon , Hong Wang
CPC classification number: C12N9/18 , C07K16/16 , C07K19/00 , C07K2319/01 , C11D3/38636 , C11D3/38681 , C12N9/14 , C12N9/2437 , C12P7/40 , C12P7/54
Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.
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9.发明申请公开(公告)号：US20130164353A1
公开(公告)日：2013-06-27
申请号：US13528201
申请日：2012-06-20
Applicant: PIERRE E. ROUVIERE , Tanja Maria Gruber , Kari A. Fosser , Stephen R. Fahnestock
Inventor： PIERRE E. ROUVIERE , Tanja Maria Gruber , Kari A. Fosser , Stephen R. Fahnestock
CPC classification number: C12N15/62 , A61K8/0241 , A61K8/64 , A61K2800/10 , A61Q5/02 , A61Q5/06 , A61Q5/065 , A61Q5/08 , A61Q5/12 , A61Q11/00 , A61Q17/04 , A61Q19/00 , C07K14/00 , C07K2319/01 , C07K2319/20 , C07K2319/74 , Y10T428/2982
Abstract: Compositions and methods using fusion peptides comprising multi-functional solubility tags are provided. The multi-functional peptidic solubility tags facilitates more efficient fusion peptide production, easier downstream processing of the fusion peptide, and may be used to provide functional surface properties when coupled to a target material.
Abstract translation: 提供了使用包含多功能溶解度标签的融合肽的组合物和方法。 多功能肽溶解性标签促进更有效的融合肽生产，更容易的融合肽的下游加工，并且当与靶物质偶联时可用于提供功能性表面性质。
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公开(公告)号：US20120317733A1
公开(公告)日：2012-12-20
申请号：US13330105
申请日：2011-12-19
Applicant: Dexter A. Chisholm , Xiumin Cui , Scott D. Cunningham , Robert DiCosimo , Stephen R. Fahnestock , Tanja Maria Gruber , Yongqing Huang , Xueping Jiang , Anju Parthasarathy , Mark S. Payne , Pierre E. Rouviere , Hong Wang
Inventor： Dexter A. Chisholm , Xiumin Cui , Scott D. Cunningham , Robert DiCosimo , Stephen R. Fahnestock , Tanja Maria Gruber , Yongqing Huang , Xueping Jiang , Anju Parthasarathy , Mark S. Payne , Pierre E. Rouviere , Hong Wang
CPC classification number: A61K8/66 , A61K8/22 , A61K8/38 , A61K8/64 , A61K38/46 , A61K38/465 , A61Q5/08 , A61Q5/10 , A61Q9/04 , A61Q15/00 , A61Q19/00 , A61Q19/02
Abstract: Disclosed herein are compositions and methods to treat hair with a peracid-based benefit agent. The peracid benefit agent can be used for hair bleaching, hair weakening, hair removal, hair waiving, hair straightening or any combination thereof. The peracid may be enzymatically generated from a carboxylic acid ester substrate using an enzyme having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a hair-binding domain, either directly or through an optional linker, may be used to target the perhydrolytic activity to the hair surface.
Abstract translation: 本文公开了用基于过酸的有益试剂治疗毛发的组合物和方法。 过酸有益剂可用于头发漂白，头发削弱，脱毛，脱毛，头发矫直或其任何组合。 在过氧源的存在下，可以使用具有过水解活性的酶（过水解酶），由羧酸酯底物酶促产生过酸。 包含直接或通过任选的接头与毛发结合结构域偶联的过水解酶的融合蛋白可用于将头发表面的水解活性靶向。
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