公开(公告)号：US09005901B2

公开(公告)日：2015-04-14

申请号：US13833365

申请日：2013-03-15

Applicant: Abbott Laboratories

Inventor： Susan Gayda ,  Qiaoqiao Ruan ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N33/53 ,  G01N35/00 ,  G01N33/543

CPC classification number: G01N35/00693 ,  G01N33/54306

Abstract: Provided herein are kits and methods for assays with internal calibration.

Abstract translation: 本文提供了用于具有内部校准的测定的试剂盒和方法。

公开(公告)号：US20220228990A1

公开(公告)日：2022-07-21

申请号：US17711375

申请日：2022-04-01

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Sylvia C. Saldana ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N21/64 ,  G01N33/543

Abstract: A method for analyzing the results of a ligand-receptor binding assay comprising the steps of: (a) providing the results of a ligand-receptor binding assay; and (b) qualifying the results of a ligand-receptor binding assay. More particularly, the ligand-receptor binding assay involves the steps of combining appropriate reagents in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is directly proportional to the amount of ligand present in the sample. Alternatively, the ligand-receptor binding assay involves the steps of combining appropriate reagents to perform a ligand-receptor binding assay in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is inversely proportional to the amount of analyte present in the sample.

公开(公告)号：US20200209250A1

公开(公告)日：2020-07-02

申请号：US16729067

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  Felicia Bogdan

IPC: G01N33/58 ,  G01N15/14 ,  G01N33/543

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US11719641B2

公开(公告)日：2023-08-08

申请号：US17711375

申请日：2022-04-01

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Sylvia C. Saldana ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N21/64 ,  G01N33/543

CPC classification number: G01N21/6456 ,  G01N33/543

Abstract: A method for analyzing the results of a ligand-receptor binding assay comprising the steps of:



(a) providing the results of a ligand-receptor binding assay; and
(b) qualifying the results of a ligand-receptor binding assay.




More particularly, the ligand-receptor binding assay involves the steps of combining appropriate reagents in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is directly proportional to the amount of ligand present in the sample. Alternatively, the ligand-receptor binding assay involves the steps of combining appropriate reagents to perform a ligand-receptor binding assay in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is inversely proportional to the amount of analyte present in the sample.

公开(公告)号：US11307141B2

公开(公告)日：2022-04-19

申请号：US16237098

申请日：2018-12-31

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Sylvia C. Saldana ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N21/64 ,  G01N33/543

Abstract: A method for analyzing the results of a ligand-receptor binding assay comprising the steps of: (a) providing the results of a ligand-receptor binding assay; and (b) qualifying the results of a ligand-receptor binding assay. More particularly, the ligand-receptor binding assay involves the steps of combining appropriate reagents in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is directly proportional to the amount of ligand present in the sample. Alternatively, the ligand-receptor binding assay involves the steps of combining appropriate reagents to perform a ligand-receptor binding assay in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is inversely proportional to the amount of analyte present in the sample.

公开(公告)号：US20240377405A1

公开(公告)日：2024-11-14

申请号：US18761492

申请日：2024-07-02

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  M. Felicia Bogdan

IPC: G01N33/58 ,  G01N15/10 ,  G01N15/14 ,  G01N33/543

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US12061200B2

公开(公告)日：2024-08-13

申请号：US16729067

申请日：2019-12-27

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Patrick J. Macdonald ,  Kerry M. Swift ,  Sergey Y. Tetin ,  Brenda Calfin ,  Zhen Lin ,  Richard Haack ,  Mark R. Pope ,  John Prostko ,  Xiaoxing Qiu ,  M. Felicia Bogdan

IPC: G01N33/53 ,  G01N15/14 ,  G01N33/543 ,  G01N33/58 ,  G01N15/10

CPC classification number: G01N33/582 ,  G01N15/14 ,  G01N33/54346 ,  G01N33/587 ,  G01N2015/1018

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

公开(公告)号：US10942179B2

公开(公告)日：2021-03-09

申请号：US16057818

申请日：2018-08-08

Applicant: ABBOTT LABORATORIES

Inventor： Barry L. Dowell ,  Susan Gayda ,  Qiaoqiao Ruan ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N33/543 ,  G01N33/53

Abstract: Provided herein are assays and kits useful for avoiding “prozone phenomenon” or “hook effect” and which expand the range of accurately measurable analyte concentrations.

公开(公告)号：US20190195798A1

公开(公告)日：2019-06-27

申请号：US16237098

申请日：2018-12-31

Applicant: Abbott Laboratories

Inventor： Qiaoqiao Ruan ,  Sylvia C. Saldana ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N21/64

CPC classification number: G01N21/6456 ,  G01N33/543

Abstract: A method for analyzing the results of a ligand-receptor binding assay comprising the steps of: (a) providing the results of a ligand-receptor binding assay; and (b) qualifying the results of a ligand-receptor binding assay. More particularly, the ligand-receptor binding assay involves the steps of combining appropriate reagents in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is directly proportional to the amount of ligand present in the sample. Alternatively, the ligand-receptor binding assay involves the steps of combining appropriate reagents to perform a ligand-receptor binding assay in which receptors attached to a solid support, a sample suspected of containing a ligand, and a conjugate comprising a label form a complex in which the label is present at a concentration that is inversely proportional to the amount of analyte present in the sample.

公开(公告)号：US10073090B2

公开(公告)日：2018-09-11

申请号：US14842834

申请日：2015-09-02

Applicant: ABBOTT LABORATORIES

Inventor： Barry L. Dowell ,  Susan Gayda ,  Qiaoqiao Ruan ,  Joseph P. Skinner ,  Sergey Y. Tetin

IPC: G01N33/53 ,  G01N33/543

CPC classification number: G01N33/54393 ,  G01N33/5306 ,  G01N33/54306

Abstract: Provided herein are assays and kits useful for avoiding “prozone phenomenon” or “hook effect” and which expand the range of accurately measurable analyte concentrations.