公开(公告)号：US12006519B2

公开(公告)日：2024-06-11

申请号：US18464955

申请日：2023-09-11

Applicant: Agilent Technologies, Inc.

Inventor： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：US20240294886A1

公开(公告)日：2024-09-05

申请号：US18652554

申请日：2024-05-01

Applicant: Agilent Technologies, Inc.

Inventor： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：US20170342483A1

公开(公告)日：2017-11-30

申请号：US15167823

申请日：2016-05-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2521/507 ,  C12Q2525/179 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2565/514

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US20240018490A1

公开(公告)日：2024-01-18

申请号：US18464955

申请日：2023-09-11

Applicant: Agilent Technologies, Inc.

Inventor： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC: C12N9/12 ,  C12Q1/6869 ,  C12P19/34

CPC classification number: C12N9/1252 ,  C12Y207/07007 ,  C12Q1/6869 ,  C12P19/34

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：US11773380B1

公开(公告)日：2023-10-03

申请号：US17894854

申请日：2022-08-24

Applicant: Agilent Technologies, Inc.

Inventor： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：USRE49207E1

公开(公告)日：2022-09-13

申请号：US16678868

申请日：2019-11-08

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US10240196B2

公开(公告)日：2019-03-26

申请号：US15167823

申请日：2016-05-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.