公开(公告)号：US09587268B2

公开(公告)日：2017-03-07

申请号：US14167513

申请日：2014-01-29

Applicant: Agilent Technologies, Inc.

Inventor： Michael Borns

IPC: C12Q1/68 ,  C12P19/34 ,  G01N33/00 ,  G01N33/566

CPC classification number: C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/137

Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

Abstract translation: 本发明涉及使用杂交的靶富集或选择核酸的组合物和方法，其可用于例如下一代测序。

公开(公告)号：USRE49207E1

公开(公告)日：2022-09-13

申请号：US16678868

申请日：2019-11-08

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US10240196B2

公开(公告)日：2019-03-26

申请号：US15167823

申请日：2016-05-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12P19/34 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US10093962B2

公开(公告)日：2018-10-09

申请号：US14868961

申请日：2015-09-29

Applicant: Agilent Technologies, Inc.

Inventor： Michael Borns

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6832

Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

公开(公告)号：US20170342483A1

公开(公告)日：2017-11-30

申请号：US15167823

申请日：2016-05-27

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Bahram Arezi ,  Michael Borns ,  Holly Hogrefe ,  Connie Hansen

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2521/507 ,  C12Q2525/179 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2565/514

Abstract: Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.

公开(公告)号：US20160017405A1

公开(公告)日：2016-01-21

申请号：US14868961

申请日：2015-09-29

Applicant: Agilent Technologies, Inc.

Inventor： Michael Borns

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/137

Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

公开(公告)号：US20150211047A1

公开(公告)日：2015-07-30

申请号：US14167513

申请日：2014-01-29

Applicant: Agilent Technologies, Inc.

Inventor： Michael Borns

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/137

Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.

Abstract translation: 本发明涉及使用杂交的靶富集或选择核酸的组合物和方法，其可用于例如下一代测序。