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公开(公告)号：US12134763B2
公开(公告)日：2024-11-05
申请号：US16930607
申请日：2020-07-16
Applicant: Archer-Daniels-Midland Company
Inventor： Charles Abbas , Dan Fanselow
Abstract: The present invention is directed to a system and method for producing an organic compound using fermentation wherein multiple components of the system are recycled within the system. The system and method allow for extraction of a high concentration of the organic compound from the fermentation broth in a continuous system that allows recycling of the biomass, aqueous fermentation broth and extraction solvents. The system and method are particularly well adapted for producing and extracting ethanol.
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公开(公告)号：US20140322766A1
公开(公告)日：2014-10-30
申请号：US14279559
申请日：2014-05-16
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Wuli Bao , Thomas Binder , Charles Abbas , Lucas Loveless
CPC classification number: C12P17/04 , C07D307/08 , C07D307/36 , C07D307/50 , C08B1/003 , C08B3/00 , C08B3/06 , C08B37/14 , C08H6/00 , C08H8/00 , C08L1/12 , C08L5/14 , C08L97/005 , C12P7/06 , C12P7/10 , C12P7/14 , C12P19/00 , C12P19/02 , C12P19/04 , C12P19/14 , C12P2201/00 , C12P2203/00 , C12Y301/00 , C12Y302/01004 , C13K1/02 , C13K13/002 , D21C3/04 , Y02E50/16 , Y02E50/17
Abstract: A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C1-C2 acid (e.g., acetic acid) with washing thereof with a C1-C2 acid miscible organic solvent, (e.g., ethyl acetate). A soluble hemicellulose and lignin enriched fraction is obtained separately from a cellulose pulp enriched fraction and lignin is removed from the soluble hemicellulose fraction. These fractions contain acylated (e.g., acetylated) cellulose and hemicellulose, which are deacylated by treatment with an alkali and/or with an acetyl esterase enzyme. The deacylated fractions are then digested with suitable cellulolytic and/or hemicellulolytic enzymes, preferably in the presence of non-ionic detergent to yield the C5 and C6 enriched syrups. Also described are method of fermentation of the syrups to make ethanol to at least 7% w/vol by separate hydrolysis and fermentation (SHF) or simultaneous hydrolysis and fermentation (SSF) methods.
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公开(公告)号：US10961549B2
公开(公告)日：2021-03-30
申请号：US15215748
申请日：2016-07-21
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Charles Abbas , Andriy Sibirny , Andriy Voronovsky , Olena Ishchuk
IPC: C07K14/39 , C07K14/395 , C12P7/06 , C12N15/81 , C12Q1/6811
Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant. Another gene from the yeast Pichia stipites was identified that encodes an orthologue protein having 37% identity to HpETT1 herein designated PsETT1 and also confers ethanol resistance to the 7E mutant.
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公开(公告)号：US20170218402A1
公开(公告)日：2017-08-03
申请号：US15215748
申请日：2016-07-21
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Charles Abbas , Andriy Sibirny , Andriy Voronovsky , Olena Ishchuk
IPC: C12P7/06 , C07K14/395 , C12N15/81
CPC classification number: C12P7/06 , C07K14/39 , C07K14/395 , C12N15/81 , C12N15/815 , C12Q1/6811 , Y02E50/17
Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant. Another gene from the yeast Pichia stipites was identified that encodes an orthologue protein having 37% identity to HpETT1 herein designated PsETT1 and also confers ethanol resistance to the 7E mutant.
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5.发明授权Cellulolytic enzyme enhancement of dry grind corn processing and ethanol production 有权纤维素分解酶增强干磨玉米加工和乙醇生产公开(公告)号：US09416377B2
公开(公告)日：2016-08-16
申请号：US14390506
申请日：2013-05-03
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Charles Abbas , Wu-Li Bao
CPC classification number: C12P7/14 , C12N9/2434 , C12P7/06 , C12P7/10 , C12P19/02 , C12P19/14 , Y02E50/16 , Y02E50/17 , Y02P20/52
Abstract: A method to increase ethanol production from a corn dry-mill process is described that comprises adding an enzyme preparation derived from Trichoderma reesei having cellulolytic activity to a saccharification process that includes conventional alpha amylase and glucoamylase. The addition of the cellulolytic enzyme decreases viscosity of the saccharified mash and can increase ethanol yield from a dry grind fermentation by as much as 10% or more. Specific characteristics are provided to show surprising and advantageous results of one particular preparation of cellulolytic enzymes from T. reesei.
Abstract translation: 描述了从玉米干磨法生产乙醇生产的方法，其包括将含有纤维素分解活性的里氏木霉的酶制剂加入到包含常规α-淀粉酶和葡糖淀粉酶的糖化过程中。 纤维素分解酶的添加降低了糖化的糊状物的粘度，并且可以将来自干研磨发酵的乙醇产量提高多达10％或更多。 提供具体特征以显示来自里氏木霉的纤维素分解酶的一种特殊制备的惊人和有利的结果。
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Patent Agency Ranking
公开(公告)号：US20160081369A1
公开(公告)日：2016-03-24
申请号：US14959747
申请日：2015-12-04
Applicant: Archer Daniels Midland Company
Inventor： Charles Abbas , Wu-Li Bao , Kyle Beery , Michael J. Cecava , Perry H. Doane , James L. Dunn , David P. Holzgraefe
Abstract: Disclosed herein are methods of treating an edible fiber source to make an animal feed with increased digestible energy. An exemplary method includes hydrolyzing the edible fiber source with an inorganic fiber hydrolyzing agent in a twin screw mixer that shears the edible fiber to a size of between 0.5 to 25 mm. The hydrolysis in the mixer occurs at pressure of about 14 psig or higher with a temperature about 100° C. to 110° C. The inorganic hydrolysis liberates a first portion of soluble carbohydrates from the edible fiber source. The inorganically hydrolyzed material is also treated (before or after) with a fiber degrading enzyme to solubilize a second portion of carbohydrates. The dually hydrolyzed material is dried to form an animal feed or feed ingredient having a soluble and insoluble carbohydrate fraction with the amount of soluble carbohydrate being at least 45% wt/wt of the total carbohydrates obtained from the edible fiber source.
Abstract translation: 本文公开了处理可食用纤维源以使具有增加的可消化能的动物饲料的方法。 示例性方法包括在双螺杆混合器中用无机纤维水解剂水解可食用纤维源，其将可食用纤维剪切成0.5至25mm的尺寸。 混合器中的水解发生在约14psig或更高的压力下，温度约为100℃至110℃。无机水解从可食用纤维源中释放出可溶性碳水化合物的第一部分。 无机水解的材料也用纤维降解酶处理（之前或之后）以溶解第二部分碳水化合物。 将双重水解的材料干燥以形成具有可溶性和不溶性碳水化合物部分的动物饲料或饲料成分，其中可溶性碳水化合物的量为从可食用纤维源获得的总碳水化合物的至少45重量％/重量。
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公开(公告)号：US20140356879A1
公开(公告)日：2014-12-04
申请号：US14358876
申请日：2013-01-11
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Charles Abbas , Andriy Sibirny , Andriy Voronovsky , Olena Ishchuk
CPC classification number: C12P7/06 , C07K14/39 , C07K14/395 , C12N15/81 , C12N15/815 , C12Q1/6811 , Y02E50/17
Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant. Another gene from the yeast Pichia stipitis was identified that encodes an orthologue protein having 37% identity to HpETT1 herein designated PsETT1 and also confers ethanol resistance to the 7E mutant.
Abstract translation: 描述赋予酵母中乙醇耐受性的基因，赋予乙醇耐性的基因和用于鉴定这些基因的突变菌株的方法。 本文命名为HpETT1的基因从酵母多形汉逊酵母中分离。 HpETT1在乙醇敏感突变体多形汉逊酵母菌株中的表达命名为7E，突变体的乙醇敏感性。 当HpETT1的多个拷贝整合到基因组中并过表达时，转化的菌株表现出对乙醇的抗性大约为10倍，并且对蛋白质错误折叠剂AZC具有抗性。 HpETT1的表达也增加了酿酒酵母中的乙醇耐受性。 HpEtt1与来自酿酒酵母的先前鉴定的蛋白质具有39％的序列同一性，表示MPE1，然而，MPE1基因不赋予7E突变体的乙醇抗性。 鉴定了来自酵母毕赤酵母属的另一种基因，其编码与本文命名为PsETT1的HpETT1具有37％同一性的直向同源蛋白，并且还赋予对7E突变体的乙醇抗性。
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8.发明申请C1-C2 Organic Acid Treatment of Lignocellulosic Biomass to Produce Acylated Cellulose Pulp, Hemicellulose, Lignin and Sugars and Fermentation of the Sugars 审中-公开木质纤维素生物质的C1-C2有机酸处理生产酰化纤维素纸浆，半纤维素，木质素和糖和发酵糖公开(公告)号：US20140322763A1
公开(公告)日：2014-10-30
申请号：US14279550
申请日：2014-05-16
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Wuli Bao , Thomas Binder , Charles Abbas , Lucas Loveless
CPC classification number: C12P17/04 , C07D307/08 , C07D307/36 , C07D307/50 , C08B1/003 , C08B3/00 , C08B3/06 , C08B37/14 , C08H6/00 , C08H8/00 , C08L1/12 , C08L5/14 , C08L97/005 , C12P7/06 , C12P7/10 , C12P7/14 , C12P19/00 , C12P19/02 , C12P19/04 , C12P19/14 , C12P2201/00 , C12P2203/00 , C12Y301/00 , C12Y302/01004 , C13K1/02 , C13K13/002 , D21C3/04 , Y02E50/16 , Y02E50/17
Abstract: A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C1-C2 acid (e.g., acetic acid) with washing thereof with a C1-C2 acid miscible organic solvent, (e.g., ethyl acetate). A soluble hemicellulose and lignin enriched fraction is obtained separately from a cellulose pulp enriched fraction and lignin is removed from the soluble hemicellulose fraction. These fractions contain acylated (e.g., acetylated) cellulose and hemicellulose, which are deacylated by treatment with an alkali and/or with an acetyl esterase enzyme. The deacylated fractions are then digested with suitable cellulolytic and/or hemicellulolytic enzymes, preferably in the presence of non-ionic detergent to yield the C5 and C6 enriched syrups. Also described are method of fermentation of the syrups to make ethanol to at least 7% w/vol by separate hydrolysis and fermentation (SHF) or simultaneous hydrolysis and fermentation (SSF) methods.
Abstract translation: 描述了从木质纤维素生物质和其发酵产物生产C5和C6糖富集糖浆的方法。 用C1-C2酸（例如乙酸）处理木质纤维素生物质，用C1-C2酸可混溶的有机溶剂（例如乙酸乙酯）洗涤。 从富含纤维素纸浆的级分分别获得可溶性半纤维素和木质素富集部分，并从可溶性半纤维素部分中除去木质素。 这些级分含有酰化（例如，乙酰化的）纤维素和半纤维素，其通过用碱和/或与乙酰酯酶进行处理而脱酰基化。 然后用合适的纤维素分解和/或半纤维素分解酶消化脱酰基化部分，优选在非离子型洗涤剂的存在下，产生富C5和C6的糖浆。 还描述了通过单独的水解和发酵（SHF）或同时水解和发酵（SSF）方法使糖浆发酵以使乙醇达到至少7％w / vol的方法。
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公开(公告)号：US20160304910A1
公开(公告)日：2016-10-20
申请号：US15101966
申请日：2014-12-05
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Andriy Sibirny , Kostyantyn V Dmytruk , Charles Abbas , Lidiia R. Murashchenko
CPC classification number: C12P7/20 , C12N9/1022 , C12N2800/102 , C12Y202/01006
Abstract: A truncated version of Saccharomyces cerevisiae IVL2 gene encoding a cytosolic form of acetolactate synthase was cloned into an expression cassette under control of a strong constitutive alcohol dehydrogenase (ADH1) promoter. The plasmid was introduced into the S. cerevisiae strain and the recombinant strain was tested for ability to overproduce glycerol under anaerobic conditions. It was shown that the recombinant strain was characterized by increased glycerol production and decreased ethanol production under anaerobic conditions.
Abstract translation: 在强组成型醇脱氢酶（ADH1）启动子的控制下，将编码细胞溶解形式的乙酰乳酸合酶的酿酒酵母酿酒酵母IVL2基因的截短版本克隆到表达盒中。 将质粒引入酿酒酵母菌株中，并测试重组菌株在厌氧条件下过量产生甘油的能力。 显示重组菌株的特征在于增加甘油产生和在厌氧条件下乙醇生产减少。
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公开(公告)号：US09428559B2
公开(公告)日：2016-08-30
申请号：US14358876
申请日：2013-01-11
Applicant: ARCHER DANIELS MIDLAND COMPANY
Inventor： Charles Abbas , Andriy Sibirny , Andriy Voronovsky , Olena Ishchuk
CPC classification number: C12P7/06 , C07K14/39 , C07K14/395 , C12N15/81 , C12N15/815 , C12Q1/6811 , Y02E50/17
Abstract: Methods of identifying genes conferring ethanol tolerance in yeasts, genes that confer ethanol tolerance, and mutant strains used to identify such genes are described. A gene herein designated HpETT1 was isolated from the yeast Hansenula polymorpha. Expression of HpETT1 in an ethanol sensitive mutant H. polymorpha strain designated 7E complimented ethanol sensitivity of the mutant. When multiple copies of the HpETT1 were integrated into the genome and overexpressed, the transformed strain demonstrated approximately 10-fold greater resistance to ethanol and resistance to the protein misfolding agent AZC. Expression of HpETT1 also increased ethanol tolerance in Saccharomyces cerevisiae. HpEtt1 has 39% sequence identity to a previously identified protein from S. cerevisiae denoted MPE1, however, the MPE1 gene does not confer ethanol resistance to the 7E mutant. Another gene from the yeast Pichia stipitis was identified that encodes an orthologue protein having 37% identity to HpETT1 herein designated PsETT1 and also confers ethanol resistance to the 7E mutant.
Abstract translation: 描述赋予酵母中乙醇耐受性的基因，赋予乙醇耐性的基因和用于鉴定这些基因的突变菌株的方法。 本文命名为HpETT1的基因从酵母多形汉逊酵母中分离。 HpETT1在乙醇敏感突变体多形汉逊酵母菌株中的表达命名为7E，突变体的乙醇敏感性。 当HpETT1的多个拷贝整合到基因组中并过表达时，转化的菌株表现出比乙醇大约10倍的抗性和对蛋白质错误折叠剂AZC的抗性。 HpETT1的表达也增加了酿酒酵母中的乙醇耐受性。 HpEtt1与来自酿酒酵母的先前鉴定的蛋白质具有39％的序列同一性，表示MPE1，然而，MPE1基因不赋予7E突变体的乙醇抗性。 鉴定了来自酵母毕赤酵母属的另一种基因，其编码与本文命名为PsETT1的HpETT1具有37％同一性的直向同源蛋白，并且还赋予对7E突变体的乙醇抗性。
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