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公开(公告)号：US09778163B2
公开(公告)日：2017-10-03
申请号：US14808720
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
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公开(公告)号：US20160018312A1
公开(公告)日：2016-01-21
申请号：US14808720
申请日：2015-07-24
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
Abstract translation: 用于分析血液样品的系统和方法，更具体地用于进行有核红细胞（nRBC）分析。 系统和方法通过荧光染色和荧光触发策略筛选血液样品，以鉴定血液样品中含有核的颗粒。 因此，基本上消除了来自未分解的红细胞（RBC）和裂解的RBC的片段的干扰。 这些系统和方法还能够开发适合于含有易碎白细胞（WBC）的样品的测定的较温和的试剂。 在一个实施方案中，系统和方法包括：（a）用专用细胞膜可渗透荧光染料染色血液样品; （b）使用荧光触发器来筛选含有核的颗粒的血液样品; 和（c）使用光散射和荧光发射的测量来区分来自WBC的nRBC。
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公开(公告)号：US20130164740A1
公开(公告)日：2013-06-27
申请号：US13720615
申请日：2012-12-19
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
IPC: C12Q1/04
CPC classification number: C12Q1/04 , G01N33/5094
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
Abstract translation: 本文提供了一种简单，可靠和准确的血液分析仪细胞分析方法。 在各个方面，所述方法通过利用荧光染料选择性地染色WBCs使得它们发射更强的荧光信号来提供红细胞（RBC）和白细胞（WBC）之间的分离和/或分化。 该方法提供了对WBC和RBCs的最佳检测限，从而允许对具有稀疏细胞浓度的样品进行分析。 为了简化体液分析，可以使用少量一种试剂来制备用于体液分析的单一稀释液。 使用本公开内容中描述的无裂解方法获得对WBC的最小损伤。
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公开(公告)号：US20210130866A1
公开(公告)日：2021-05-06
申请号：US17002089
申请日：2020-08-25
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
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公开(公告)号：US10481072B2
公开(公告)日：2019-11-19
申请号：US15704974
申请日：2017-09-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
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Patent Agency Ranking
公开(公告)号：US09970045B2
公开(公告)日：2018-05-15
申请号：US13720615
申请日：2012-12-19
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
CPC classification number: C12Q1/04 , G01N33/5094
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
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公开(公告)号：US20180128732A1
公开(公告)日：2018-05-10
申请号：US15704974
申请日：2017-09-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Marilou Coleman , Emily H. Lin , Michael R. Buhl , Giacomo Vacca
CPC classification number: G01N15/1434 , G01N15/1459 , G01N15/147 , G01N21/6428 , G01N33/49 , G01N33/80 , G01N2015/0069 , G01N2015/008 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , G01N2015/1488 , G01N2021/6439
Abstract: Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
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公开(公告)号：US11193927B2
公开(公告)日：2021-12-07
申请号：US16330289
申请日：2017-09-07
Applicant: Abbott Laboratories
Inventor： Wenjing Li , Emily H. Lin , Jiong Wu
Abstract: Methods, devices, and systems for automated cellular analysis of a body fluid sample are disclosed. The methods, devices, and systems apply watershed transform to data, generated by flowing a body fluid sample through a flow cytometer, to determine threshold(s) to be used for analysis of the data.
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公开(公告)号：US10774359B2
公开(公告)日：2020-09-15
申请号：US15979230
申请日：2018-05-14
Applicant: Abbott Laboratories
Inventor： Jiong Wu , Emily H. Lin , Jihping Yang
Abstract: Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure.
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公开(公告)号：US20190204301A1
公开(公告)日：2019-07-04
申请号：US16330289
申请日：2017-09-07
Applicant: Abbott Laboratories
Inventor： Wenjing Li , Emily H. Lin , Jiong Wu
CPC classification number: G01N33/5094 , G01N33/5002 , G01N33/52
Abstract: Methods, devices, and systems for automated cellular analysis of a body fluid sample are disclosed. The methods, devices, and systems apply watershed transform to data, generated by flowing a body fluid sample through a flow cytometer, to determine threshold(s) to be used for analysis of the data.
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