公开(公告)号：US20160010138A1

公开(公告)日：2016-01-14

申请号：US14772376

申请日：2014-03-17

Applicant: ACCELERATE DIAGNOSTICS, INC.

Inventor： Alena Shamsheyeva ,  David Howson ,  Steven W. Metzger

IPC: C12Q1/18 ,  G06F19/12

CPC classification number: C12Q1/18 ,  B01L3/502715 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2300/024 ,  B01L2300/0864 ,  B01L2300/0867 ,  C12M41/36 ,  C12Q1/02 ,  G16B5/00

Abstract: This disclosure is related to systems and methods for rapid determination of microorganism growth and antimicrobial agent susceptibility and/or resistance.

Abstract translation: 本公开涉及用于快速测定微生物生长和抗微生物剂敏感性和/或抗性的系统和方法。

公开(公告)号：US20140323340A1

公开(公告)日：2014-10-30

申请号：US14329387

申请日：2014-07-11

Applicant: Accelerate Diagnostics, Inc.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: C12Q1/18

CPC classification number: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/5438 ,  G01N33/561

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

Abstract translation: 本发明涉及将微生物移动到表面，其中它们在存在和不存在抗微生物剂的情况下生长，并且通过在两个条件下随时间监测微生物的生长，可以确定它们对抗微生物剂的敏感性。 微生物可通过电泳，离心或过滤移至表面。 当运动涉及电泳时，氧化和还原剂的存在降低了可以产生电泳力的电压，并允许可以检测目标的更宽范围的方法。 监测可以包括光学检测，并且最方便地包括检测单个微生物。 微生物可以被染色以便提供关于它们对抗微生物剂的反应的信息。

公开(公告)号：US10254204B2

公开(公告)日：2019-04-09

申请号：US15586132

申请日：2017-05-03

Applicant: Accelerate Diagnostics, Inc.

Inventor： Landon Prisbrey ,  Elise Blackmore ,  Kenneth R. Hance ,  Steven W. Metzger ,  Kevin Marshall

IPC: A61K39/12 ,  G01N1/40 ,  B03C5/00 ,  C12N13/00 ,  G01N33/49 ,  C12M1/00 ,  C12Q1/02 ,  C12Q1/24 ,  G01N1/34 ,  G02B21/36 ,  C12N7/00

Abstract: Methods and systems for purifying one or more microbial cells and/or viruses from a biological sample are provided. The biological sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the microbial cells and/or viruses in the well. The microbial cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the microbial cells and/or viruses are retained in the well by embedding in the medium. The medium including the embedded microbial cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface. In some examples, a biological sample containing contaminants and one or more microbial cells is introduced to a well disposed in a porous filter medium, wherein the porous filter medium includes pores smaller than the one or more microbial cells, thereby preventing the one or more microbial cells from entering the porous filter medium.

公开(公告)号：US20150337351A1

公开(公告)日：2015-11-26

申请号：US14720588

申请日：2015-05-22

Applicant: ACCELERATE DIAGNOSTICS, INC.

Inventor： Steven W. Metzger

IPC: C12Q1/06 ,  C12Q1/08 ,  C12Q1/10 ,  C12Q1/04 ,  C12Q1/14

CPC classification number: C12Q1/06 ,  C12Q1/02 ,  C12Q1/025 ,  C12Q1/045 ,  C12Q1/08 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/18 ,  G01N33/5436

Abstract: The present disclosure is related to methods for immobilizing microorganisms to produce an immobilized microorganism sample for detection with a detection system. Compositions for immobilizing microorganisms are also disclosed.

Abstract translation: 本公开涉及用于固定微生物以产生用于用检测系统检测的固定化微生物样品的方法。 还公开了用于固定微生物的组合物。

公开(公告)号：US08895255B1

公开(公告)日：2014-11-25

申请号：US13763446

申请日：2013-02-08

Applicant: Accelerate Diagnostics, Inc.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/53

CPC classification number: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  C12Q1/18 ,  G01N33/5438 ,  G01N33/561

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

Abstract translation: 本发明涉及将微生物移动到表面，其中它们在存在和不存在抗微生物剂的情况下生长，并且通过在两个条件下随时间监测微生物的生长，可以确定它们对抗微生物剂的敏感性。 微生物可通过电泳，离心或过滤移至表面。 当运动涉及电泳时，氧化和还原剂的存在降低了可以产生电泳力的电压，并允许可以检测目标的更宽范围的方法。 监测可以包括光学检测，并且最方便地包括检测单个微生物。 微生物可以被染色以便提供关于它们对抗微生物剂的反应的信息。

公开(公告)号：US20240385099A1

公开(公告)日：2024-11-21

申请号：US18692433

申请日：2022-09-15

Applicant: Accelerate Diagnostics, Inc.

Inventor： Landon Prisbey ,  Steven W. Metzger ,  Oleg Gusyatin

IPC: G01N15/0227 ,  C12Q1/04 ,  C12Q1/18 ,  G01N15/02 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1433 ,  G01N15/1434

Abstract: Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

公开(公告)号：US11054420B2

公开(公告)日：2021-07-06

申请号：US15827187

申请日：2017-11-30

Applicant: Accelerate Diagnostics, Inc.

Inventor： David A. Goldberg ,  David C. Howson ,  Steven W. Metzger ,  Daniel A. Buttry ,  Steven Scott Saavedra

IPC: G01N33/569 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y20/00 ,  G01N33/543 ,  G01N33/561 ,  C12Q1/18

Abstract: The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.

公开(公告)号：US20210008541A1

公开(公告)日：2021-01-14

申请号：US16908516

申请日：2020-06-22

Applicant: Accelerate Diagnostics, Inc.

Inventor： Kevin Marshall ,  Austin Ashby ,  Josh Harrison ,  Steven W. Metzger ,  Wayne Showalter ,  Erik Molina ,  Jamie Roberson

IPC: B01L3/02

Abstract: Systems and methods for automated biological sample preparation for use in rapid identification and antimicrobial susceptibility testing of microorganisms, such as bacteria and fungi, are provided.

公开(公告)号：US10669566B2

公开(公告)日：2020-06-02

申请号：US16007071

申请日：2018-06-13

Applicant: Accelerate Diagnostics, Inc.

Inventor： William L. Richards ,  Austin Ashby ,  Matthew Ketterer ,  Kevin Marshall ,  Josh Harrison ,  Matthew Mette ,  Paul Richards ,  Wayne Showalter ,  Jasmin Cote ,  Steven W. Metzger ,  Ken Hance ,  Meghan Mensack ,  Carlos Michel ,  Elke Allers ,  Dulini Gamage ,  Landon Prisbrey ,  Oleg Gusyatin ,  Alena Shamsheyeva ,  Ben Turng ,  Andrew Ghusson ,  Kurt Reinhardt ,  Phillip C. Halbert ,  Solene Bourgeois

IPC: C12Q1/02 ,  G02B7/28 ,  G01N15/06 ,  G01N21/64 ,  B01L3/00 ,  C12Q1/04 ,  G01N1/38 ,  G01N35/02 ,  G01N35/10 ,  H04N5/232 ,  G01N35/04 ,  G01N35/00

Abstract: A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing.

公开(公告)号：US10253355B2

公开(公告)日：2019-04-09

申请号：US15283922

申请日：2016-10-03

Applicant: Accelerate Diagnostics, Inc.

Inventor： William L. Richards ,  Austin Ashby ,  Matthew Ketterer ,  Kevin Marshall ,  Josh Harrison ,  Matthew Mette ,  Paul Richards ,  Wayne Showalter ,  Jasmin Cote ,  Phillip C. Halbert ,  Solene Bourgeois ,  Steven W. Metzger ,  Ken Hance ,  Meghan Mensack ,  Carlos Michel ,  Elke Allers ,  Dulini Gamage ,  Landon Prisbrey ,  Oleg Gusyatin ,  Alena Shamsheyeva ,  Ben Turng ,  Andrew Ghusson ,  Kurt Reinhardt

IPC: C12Q1/6841 ,  C12Q1/02 ,  G02B7/28 ,  G01N21/64 ,  G01N35/02 ,  H04N5/232 ,  G01N35/00 ,  C12M1/34 ,  G01N35/10 ,  B01L3/00

Abstract: A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing. Automated quality control test components and methods of their use are also disclosed.