公开(公告)号：US09771575B2

公开(公告)日：2017-09-26

申请号：US14745174

申请日：2015-06-19

Applicant: Agilent Technologies, Inc.

Inventor： Alexander Belyaev ,  Nicholas M. Sampas ,  Alicia Scheffer-Wong

IPC: C12Q1/68 ,  C12N15/10 ,  C12N9/12 ,  C07H21/04

CPC classification number: C12N15/1065 ,  C12Q2521/327 ,  C12Q2563/179

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.

公开(公告)号：US20160369266A1

公开(公告)日：2016-12-22

申请号：US14745174

申请日：2015-06-19

Applicant: Agilent Technologies, Inc.

Inventor： Alexander Belyaev ,  Nicholas M. Sampas ,  Alicia Scheffer-Wong

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12Q2521/327 ,  C12Q2563/179

Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.

Abstract translation: 本公开提供了使用含条形码的转座酶复合物的微阵列条形码编码多个DNA样品的方法。 在一些实施方案中，DNA样品和转座酶复合物存在于相对底物表面上的水性液滴中，这允许单个DNA样品液滴与单个转座酶复合物液滴组合。 组合液滴中的条形码DNA可用于任何数量的目的，包括用于扩增和测序的模板。