公开(公告)号：US08970841B2

公开(公告)日：2015-03-03

申请号：US13513786

申请日：2010-12-03

Applicant: Alexander Chekalyuk

Inventor： Alexander Chekalyuk

IPC: G01J3/30 ,  G01J3/28 ,  G01N21/25 ,  G01N21/64 ,  G01J3/02 ,  G01J3/44 ,  G01N21/65 ,  G01N21/63

CPC classification number: G01N21/6408 ,  G01J3/02 ,  G01J3/0264 ,  G01J3/4406 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/635 ,  G01N2021/6419

Abstract: An Advanced Laser Fluorometer (ALF) can combine spectrally and temporally resolved measurements of laser-stimulated emission (LSE) for characterization of dissolved and particulate matter, including fluorescence constituents, in liquids. Spectral deconvolution (SDC) analysis of LSE spectral measurements can accurately retrieve information about individual fluorescent bands, such as can be attributed to chlorophyll-a (Chl-a), phycobiliprotein (PBP) pigments, or chromophoric dissolved organic matter (CDOM), among others. Improved physiological assessments of photosynthesizing organisms can use SDC analysis and temporal LSE measurements to assess variable fluorescence corrected for SDC-retrieved background fluorescence. Fluorescence assessments of Chl-a concentration based on LSE spectral measurements can be improved using photo-physiological information from temporal measurements. Quantitative assessments of PBP pigments, CDOM, and other fluorescent constituents, as well as basic structural characterizations of photosynthesizing populations, can be performed using SDC analysis of LSE spectral measurements.

Abstract translation: 先进的激光荧光计（ALF）可以结合光谱和时间分辨的激光激发辐射测量（LSE），用于表征液体中溶解和颗粒物质，包括荧光成分。 LSE光谱测量的光谱去卷积（SDC）分析可以准确检索关于单个荧光带的信息，例如可归因于叶绿素a（Chl-a），藻胆蛋白（PBP）颜料或发色团溶解有机物（CDOM）），其中 其他。 改进的光合生物生理学评估可以使用SDC分析和时间LSE测量来评估针对SDC检索的背景荧光校正的可变荧光。 基于LSE光谱测量的Chl-a浓度的荧光评估可以使用来自时间测量的光生物学信息来改善。 可以使用LSE光谱测量的SDC分析来进行PBP颜料，CDOM和其他荧光成分的定量评估，以及光合作用群体的基本结构表征。

公开(公告)号：US20120324986A1

公开(公告)日：2012-12-27

申请号：US13513786

申请日：2010-12-03

Applicant: Alexander Chekalyuk

Inventor： Alexander Chekalyuk

IPC: G01N30/00 ,  G01N21/64

CPC classification number: G01N21/6408 ,  G01J3/02 ,  G01J3/0264 ,  G01J3/4406 ,  G01N21/6486 ,  G01N21/65 ,  G01N2021/635 ,  G01N2021/6419

Abstract: An Advanced Laser Fluorometer (ALF) can combine spectrally and temporally resolved measurements of laser-stimulated emission (LSE) for characterization of dissolved and particulate matter, including fluorescence constituents, in liquids. Spectral deconvolution (SDC) analysis of LSE spectral measurements can accurately retrieve information about individual fluorescent bands, such as can be attributed to chlorophyll-a (Chl-a), phycobiliprotein (PBP) pigments, or chromophoric dissolved organic matter (CDOM), among others. Improved physiological assessments of photosynthesizing organisms can use SDC analysis and temporal LSE measurements to assess variable fluorescence corrected for SDC-retrieved background fluorescence. Fluorescence assessments of Chl-a concentration based on LSE spectral measurements can be improved using photo-physiological information from temporal measurements. Quantitative assessments of PBP pigments, CDOM, and other fluorescent constituents, as well as basic structural characterizations of photosynthesizing populations, can be performed using SDC analysis of LSE spectral measurements.

Abstract translation: 先进的激光荧光计（ALF）可以结合光谱和时间分辨的激光激发辐射测量（LSE），用于表征液体中溶解和颗粒物质，包括荧光成分。 LSE光谱测量的光谱去卷积（SDC）分析可以准确检索关于单个荧光带的信息，例如可归因于叶绿素a（Chl-a），藻胆蛋白（PBP）颜料或发色溶解有机物（CDOM）），其中 其他。 改进的光合生物生理学评估可以使用SDC分析和时间LSE测量来评估针对SDC检索的背景荧光校正的可变荧光。 基于LSE光谱测量的Chl-a浓度的荧光评估可以使用来自时间测量的光生物学信息来改善。 可以使用LSE光谱测量的SDC分析来进行PBP颜料，CDOM和其他荧光成分的定量评估，以及光合作用群体的基本结构表征。